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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (11): 1164-1169.doi: 10.3736/jcim20081111

• Original Experimental Research • Previous Articles     Next Articles

Effects of Tangxinping Capsule on angiotensin Ⅱ and its type 1 receptor and myocardial ultrastructure in diabetic rats

Min Li1, Qing Ni2, Xiao-yan Chu2, Zhao-li Wang2, Lan Lin2()   

  1. 1. Department of Molecular Biology, Guang’anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053, China;
    2. Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China
  • Received:2008-03-11 Online:2008-11-20 Published:2008-11-15


To investigate the protective effects of Tangxinping Capsule, a compound traditional Chinese herbal medicine, on diabetic cardiomyopathy in rats.


The model of diabetes mellitus was induced by streptozotocin (STZ) injection in rats. And the rats with diabetes mellitus were randomly divided into untreated group, metformin group, captopril group, and low-, medium- and high-dose Tangxinping group; the other ten normal rats were used as normal controls. After eight-week treatment, all rats were sacrificed to detect angiotensin Ⅱ (Ang Ⅱ) protein content and angiotensin Ⅱ type 1 receptor (AT1R) mRNA expression in myocardial tissues by radioimmunoassay and reverse transcription polymerase chain reaction (RT-PCR) respectively. The ultrastructural change in myocardial tissues was detected by transmission electron microscopy.


The results of radioimmunoassay and RT-PCR showed that the content of Ang Ⅱ protein and the expression of AT1R mRNA in myocardial tissue of the untreated group was significantly increased and more than those in normal control (P<0.05, P<0.01). Only the content of Ang Ⅱ and expression of AT1R mRNA in high-dose Tangxinping group and captopril group were significantly lower than those in the untreated group (P<0.05, P<0.01). The transmission electron microscopy showed that volume of mitochondrion in myocardial tissue in the untreated group was bigger than that in normal control group. Also the volume of mitochondrion was improved in 5 treated groups, but had no significant difference when compared with the untreated group.


Tangxinping may adjust the disorder of renin-angiotensin system to protect myocardial tissue in rats with cardiomyopathy due to diabetes.

Key words: Tangxinping capsule, diabetes complications, angiotensin H, angiotensin H type 1 receptor, cardiomyopathy, rats

CLC Number: 

  • R587.23

Table 1

Contents of blood glucose and Ang Ⅱ and expression of AT1R mRNA in myocardial tissue in different groups(x±s)"

Group n Blood glucose (mmol/L) Ang Ⅱ (pg/mg) AT1R mRNA
Normal control 10 6.63±1.29 61.42±13.47 0.13±0.01
Untreated 10 33.54±2.54** 80.31±17.32** 0.46±0.12**
Captopril 10 27.99±5.27** 63.26±12.68 0.16±0.07△△
Metformin 10 24.98±3.01**△△ 77.45±19.23** 0.33±0.15*
Low-dose TXPC 10 26.24±4.41** 78.92±13.59** 0.38±0.12*
Medium-dose TXPC 10 25.32±4.31** 74.44±15.21* 0.33±0.10*
High-dose TXPC 10 21.24±4.27**△△ 67.89±13.52 0.23±0.15

Figure 1

Gel electrophoretogram of AT1R mRNA expression in myocardial tissue in different groups 1, 3, 5, 7, 9, 11, 13: GAPDH; 2, 4, 6, 8, 10, 12, 14: AT1R; 1, 2: Normal control group; 3, 4: Untreated group; 5, 6: High-dose TXPC group; 7, 8: Medium-dose TXPC group; 9, 10: Low-dose TXPC group; 11, 12: Captopril group; 13, 14: Metformin group; 15: Negative control."

Figure 2

Ultrastructure of mitochondrion in myocardial tissue (Transmission electron microscopy, ×10 000) A: Normal control group; B: Untreated group; C: Captopril group; D: Metformin group; E: Low-dose TXPC group; F: Medium-dose TXPC group; G: High-dose TXPC group."

Figure 3

Ultrastructure of capillary vessel membrane in myocardial tissue (Transmission electron microscopy, ×10 000) A: Normal control group; B: Untreated group; C: Captopril group; D: Metformin group; E: Low-dose TXPC group; F: Medium-dose TXPC group; G: High-dose TXPC group."

[1] Huang B, Liu XZ, Pang DB . Study on diabetic model in rats induced by streotozotocin in different ways[J]. Jinzhou Yi Xue Yuan Xue Bao, 2003,24(1):19-21
doi: 10.3969/j.issn.1674-0424.2003.01.006
黄波, 刘学政, 庞东渤 . 不同途径注射链脲佐菌素致大鼠糖尿病模型的研究[J]. 锦州医学院学报, 2003,24(1):19-21
doi: 10.3969/j.issn.1674-0424.2003.01.006
[2] McDonald KM, Garr M, Carlyle PF , et al. Relative effects of alpha 1-adrenoceptor blockade, converting enzyme inhibitor therapy, and angiotensin Ⅱ subtype 1 receptor blockade on ventricular remodeling in the dog[J]. Circulation, 1994,90(6):3034-3046
doi: 10.1161/01.CIR.90.6.3034
[3] Metsarinne KP, Helin KH, Saijonmaa O , et al. Tissue-specific regulation of angiotensin-converting enzyme by angiotensin Ⅱ and losartan in the rat[J].Blood Press, 1996,5(6):363-370
doi: 10.3109/08037059609078076
[4] Haywood GA, Gullestad L, Katsuya T , et al. AT1 and AT2 angiotensin receptor gene expression in human heart failure[J]. Circulation, 1997,95(5):1201-1206
doi: 10.1161/01.CIR.95.5.1201
[5] Jennings G . New definitions in cardiovascular risk management: is it time for angiotensin Ⅱ receptor blockers to become first-line medication?[J]. Eur Heart J Suppl, 2003,5(Suppl F):F3-F11
doi: 10.1016/S1520-765X(03)90010-1
[6] Kaprielian RR, Dupont E, Hafizi S , et al. Angiotensin Ⅱ receptor type 1 mRNA is unregulated in atria of patients with end-stage heart failure[J]. J Mol Cell Cardiol, 1997,29(8):2299-2304
doi: 10.1006/jmcc.1997.0458
[7] Clozcl M, Kuhn H, Hefti F . Effects of angiotensin converting enzyme inhibitors and of hydralazine on endothelial function in hypertensive rats[J]. Hypertension, 1990,16(5):532-540
doi: 10.1161/01.HYP.16.5.532
[8] Kajstura J, Fiordaliso F, Andreoli AM , et al. IGF-1 overexpression inhibits the development of diabetic cardiomyopathy and angiotensin Ⅱ-mediated oxidative stress[J]. Diabetes, 2001,50(6):1414-1424
doi: 10.2337/diabetes.50.6.1414
[9] Fiordaliso F, Li B, Latini R , et al. Myocyte death in streptozotocin-induced diabetes in rats in angiotensin Ⅱ-dependent[J]. Lab Invest, 2000,80(4):513-527
doi: 10.1038/labinvest.3780057 pmid: 10780668
[10] Jing YL, Tian H, Pan CY , et al. The effect of angiotensin Ⅱ on cardiomyopathy of STZ diabetic rats and the protective effect of captopril[J]. Zhongguo Tang Niao Bing Za Zhi, 2000,8(5):298-300
doi: 10.3321/j.issn:1006-6187.2000.05.013
景雅莉, 田慧, 潘长玉 , 等. 血管紧张素Ⅱ对实验性糖尿病大鼠心肌病变的影响及卡托普利的保护作用[J]. 中国糖尿病杂志, 2000,8(5):298-300
doi: 10.3321/j.issn:1006-6187.2000.05.013
[11] Yang J, Xue C, Hu G . Effects of enalapril on plasma Ang Ⅱ level and the expression of AT1 in blood vessel and kidney of type 2 diabetic rats[J]. Yao Xue Xue Bao, 2005,40(3):208-212
doi: 10.3321/j.issn:0513-4870.2005.03.004
杨俭, 薛春, 胡刚 . 依那普利对2型糖尿病大鼠血浆Ang Ⅱ水平及血管、肾脏AT1受体表达的影响[J]. 药学学报, 2005,40(3):208-212
doi: 10.3321/j.issn:0513-4870.2005.03.004
[12] Shi ZM, Qian XM, Xu RJ , et al. A study of angiotensin-Ⅱ and myocardial ultrastructure in experimental diabetic rats[J]. Zhongguo Tang Niao Bing Za Zhi, 1996,4(1):28-30
施志明, 钱晓明, 许瑞吉 , 等. 实验性糖尿病大鼠心肌ATⅡ和心肌超微结构的研究[J]. 中国糖尿病杂志, 1996,4(1):28-30
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