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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (12): 1367-1372.doi: 10.3736/jcim20111214

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Yiqi Huayu formula on substance P expression in skin ulcers of rats with diabetes mellitus

Yun-fei Wang, Hua-fa Que()   

  1. Institute of Traditional Chinese Medicine Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2011-07-26 Accepted:2011-08-24 Online:2011-12-20 Published:2011-12-15

Objective: To investigate the effects of Chinese herbs for replenishing qi (Yiqi) and dissolving stasis (Huayu) on substance P expression in granulation tissue of skin ulcers in rats with diabetes mellitus.
Methods: One hundred male Sprague-Dawley rats with back full-thickness skin lesion were randomly divided into five groups: ulcer control group, diabetic ulcer group, Yiqi Huayu herb group, Yiqi herb group, and Huayu herb group. Except ulcer control group, the other rats were also injected streptozotocin to induce diabetes mellitus. Rats in each group were given normal saline or herbal drugs for 15 d and the wound healing rate of the rats was observed on days 8 and 15, respectively. After treatment, the expression of substance P in granulation tissues of the rats was tested with immunohistochemical method and the immunohistochemical index was calculated.
Results: On the 8th and 15th days after treatment, the wound healing rate of the ulcer control group was higher than that of the diabetic ulcer group (P<0.01); the wound healing rates of all the treated groups were higher than that of the diabetic ulcer group (P<0.05); the wound healing rate of the Yiqi Huayu herb group was higher than those of the Yiqi herb group and the Huayu herb group (P<0.05). Compared with the diabetic ulcer group, the expression of substance P of the ulcer control group was increased (P<0.05); the expressions of substance P of the Yiqi Huayu herb group and the Huayu herb group were higher than that of the diabetic ulcer group (P<0.05).
Conclusion: Yiqi Huayu herbs can promote wound healing in rats with diabetes mellitus by up-regulating the expression of substance P.

Key words: diabetes complications, skin ulcer, drugs, Chinese herbal, substance P

Table 1

Wound healing rate of rats on days 8 and 15 (x±s, %)"

Group n Wound healing rate
Day 8 Day 15
Ulcer control 20 38.56±1.53 91.55±2.61
Diabetic ulcer 20 16.45±0.82** 65.53±1.82**
Yiqi herb 20 18.41±1.07*△▲ 74.41±1.07*△▲
Huayu herb 20 18.20±0.50*△▲ 76.20±1.50*△▲
Yiqi Huayu herb 20 20.01±1.40*△ 84.18±2.30*△△

Table 2

Immunohistochemical index of substance P in ulcer tissues of rats with diabetes mellitus (x±s, ×104)"

Group n Immunohistochemical index of substance P
Ulcer control 20 4.264 8±0.185 7
Diabetic ulcer 20 3.552 8±0.259 7
Yiqi herb 20 3.826 9±0.151 1
Huayu herb 20 4.381 3±0.233 1
Yiqi Huayu herb 20 4.280 8±0.358 0

Figure 1

Expression of substance P in granulation tissue of skin ulcer wound in rats observed by immunohistochemical SABC method (Light microscopy, ×100) A:Ulcer control group; B: Diabetic ulcer group; C: Yiqi herb group; D: Huayu herb group; E: Yiqi Huayu herb group."

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