川芎嗪对心室快速起搏心力衰竭实验犬心房颤动及心房纤维化的影响

doi:10.3736/jcim20060110

Abstract

Objective

To explore the effects of tetramethylpyrazine (TMP) on fibrosis of atrial tissue and atrial fibrillation in a canine model of congestive heart failure (CHF) induced by ventricular tachypacing.

Methods

Twenty-one healthy mongrel dogs were randomly divided into three groups, which were normal control group, untreated group and TMP-treated group. Atrial fibrillation (AF) was induced by burst of atrial pacing, after the canine model of CHF was established. The atrial tissues were sampled and stained with Mallory’s trichromic stains, then the fibrosis in the atrial tissues was analyzed. The left ventricular ejection fraction (LVEF) was evaluated by echocardiography. The levels of angiotensin Ⅱ (AngⅡ), aldosterone (ALD), amino-terminal peptide of type Ⅲ procollagen (PⅢNP),laminin (LN) and hyaluronic acid (HA) in peripheral blood were examined by radioimmunoassay.

Results

The LVEF was significantly decreased in the untreated group as compared with that in the normal control group (P＜0.01）, while the frequencies of AF and sustaining AF were significantly increased and the AF duration was obviously prolonged in the untreated group as compared with those in the normal control group (P＜0.01）. The fibrosis degree in the left or right atrial tissue in the untreated group was more serious than that in the normal control group (P＜0.01). The AF duration was positively correlated with the fibrosis degree in the left atrial tissue (r=0.84, P=0.018). The levels of AngⅡ, ALD, PⅢNP and HA in peripheral blood were significantly higher in the untreated group than those in the normal control group (P＜0.05 or P＜0.01). The level of AngⅡ was positively correlated with the level of ALD in peripheral blood (r=0.759, P=0.048). The LVEF and the frequency of sustaining AF were both significantly improved in the TMP-treated group as compared with those in the untreated group (P＜0.05). The fibrosis in the left or right atrial tissue in the untreated group was more serious than that in the untreated group (P＜0.01). The levels of AngⅡ and PⅢNP in peripheral blood were also markedly higher in the TMP-treated group than those in the untreated group (P＜0.05, P＜0.01).

Conclusion

Tetramethylpyrazine has the effect of reducing the fibrosis degree of atrial tissue in dogs with CHF, and this efficacy may be related to the mechanism of decreasing the frequency of AF and shortening the AF duration.

Key words: TetramethylPyrazine, Heart failure, Congestive, Atrial fibrillation, Animal experimentation

CLC Number:

R286.2

Ya-zhou Lin, Chun-xuan Xu, Yu-lian Deng, Lin Chen, Hai Huang, Jian Du. Effects of tetramethylpyrazine on fibrosis of atrial tissue and atrial fibrillation in a canine model of congestive heart failure induced by ventricular tachypacing. Journal of Chinese Integrative Medicine, 2006, 4(1): 35-38.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20060110

http://www.jcimjournal.com/EN/Y2006/V4/I1/35

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References 12

[1]	Nattel S.New ideas about atrial fibrillation 50 years on[J].Nature,2002,415(6868):219-226
[2]	Allessie MA, Boyden PA, Camm AJ, et al.Pathophysiology and prevention of atrial fibrillation[J].Circulation,2001,103(5):769-777 doi: 10.1161/01.CIR.103.5.769 pmid: 11156892
[3]	侯建平. 黄芪注射液与川芎嗪注射液联用治疗慢性充血性心力衰竭43例疗效分析[J].实用心脑肺血管病杂志,2000,8(1):49-50
[4]	黄焱, 陈少强, 张更, 等.川芎嗪联合氨基胍对糖尿病大鼠肾脏血管内皮细胞生长因子表达的影响[J].中西医结合学报,2004,2(1):39-41
	Huang Y, Chen SQ, Zhang G, et al.Effect of tetromethylpyrazine and aminoguanidine on expression of vascular endothelial growth factor in kidney of diabetic rats[J].Zhong Xi Yi Jie He Xue Bao,2004,2(1):39-41
[5]	Gronefeld GC, Hohnloser SH.Heart failure complicated by atrial fibrillation: mechanistic, prognostic, and therapeutic implications[J].J Cardiovasc Pharmacol Ther,2003,8(2):107-113
[6]	Verheule S, Wilson E, Everett T 4th, et al.Alterations in atrial electrophysiology and tissue structure in a canine model of chronic atrial dilatation due to mitral regurgitation[J].Circulation,2003,107(20):2615-2622
[7]	张建成, 黄从新, 邓玉莲, 等.心房颤动患者L型钙通道电流改变的分子基础[J].中华心血管病杂志,2002,30(8):460-463 doi: 10.3760/j:issn:0253-3758.2002.08.004
[8]	张建成, 黄从新, 邓玉莲, 等.心房颤动患者离子通道蛋白质重构的研究[J].中国循环杂志,2002,17(4):297-300 doi: 10.3969/j.issn.1008-0074.2009.06.05
[9]	Gonzalez A, Lopez B, Diez J.Fibrosis in hypertensive heart disease: role of the renin-angiotensin-aldosterone system[J].Med Clin North Am,2004,88(1):83-97 doi: 10.1016/S0025-7125(03)00125-1 pmid: 14871052
[10]	郭自强, 牛福玲, 朱陵群, 等.川芎嗪对血管紧张素Ⅱ致心肌细胞肥大的影响[J].北京中医药大学学报,2001,24(3):32-34
[11]	Kumagai K, Nakashima H, Urata H, et al.Effects of angiotensin Ⅱ type 1 receptor antagonist on electrical and structural remodeling in atrial fibrillation[J].J Am Coll Cardiol,2003,41(12):2197-2204
[12]	商庆新, 张涤生, 关文祥, 等.丹参和川芎嗪对瘢痕成纤维细胞DNA含量及细胞周期进程的影响[J].中国修复重建外科杂志,1998,12(6):325-328

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Group	n	Frequency of AF	Frequency of sustaining AF	AF duration (?x±s, s)	LVEF (?x±s, %)
Normal control	7	2/7	0/7	0.6±0.6	67.4±6.0
Untreated	7	7/7**	5/7**	462.1±181.4**	29.2±7.8**
TMP-treated	7	6/7**	3/7**^△	340.6±162.6**	40.7±7.5**^△

Group	n	Fibrosis degree of	Fibrosis degree of
Group	n	right atrial tissue	left atrial tissue
Normal control	7	2.88±0.45	3.05±0.46
Untreated	7	8.36±1.46**	11.25±1.76**
TMP-treated	7	5.64±0.99**^△△	5.46±1.14**^△△

Group	n	AngiotensinⅡ	Aldosterone	PⅢNP	LN	HA
Normal control	7	66.5±22.8	68.6±19.8	14.7±3.0	58.6±12.5	85.1±16.6
Untreated	7	96.3±20.1**	88.8±20.3*	19.3±3.8**	56.5±8.4	103.2±19.1*
TMP-treated	7	77.5±10.2^△	82.5±24.9	13.4±3.6^△△	56.5±8.4	99.6±24.9

Effects of tetramethylpyrazine on fibrosis of atrial tissue and atrial fibrillation in a canine model of congestive heart failure induced by ventricular tachypacing

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