Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (3): 285-288.doi: 10.3736/jcim20060313

• Original Experimental Research • Previous Articles     Next Articles

Inhibiting effects of root of Mallotus apelta on duck hepatitis B virus

Shu XU, Zhi-ping Lv, Hong-bing Cai, Xiao-gang Zhang, Qiang Liu, Yan Tan   

  1. School of Traditional Chinese Medicine, Nanfang Medical University, Guangzhou, Guangdong Province 510515, China
  • Online:2006-05-31 Published:2006-05-15


To evaluate the inhibiting effects of the root of Mallotus apelta (Lour.) Muell.-Arg. on duck hepatitis B virus (D-HBV) in vivo.


Forty nestling ducks with congenitally infection of D-HBV detected by PCR were randomly divided into five groups: untreated group, lamivudine-treated group, and high-, medium- and low-dose root of Mallotus apelta-treated groups. The ducks in the lamivudine-treated group were fed lamivudine with a dose of 50 mg/kg once. Ducks in the three-dose Mallotus apelta-treated groups were treated with different doses of decoction of this herbal medicine for 21 days respectively. The serum content of D-HBV DNA was determined by quantitative real-time PCR technique before and 7 days after the treatment, and on the 7th, 14th and 21st day of the treatment. Liver biopsy was also executed before and after the treatment to observe the histopathological changes.


Lamivudine showed a rapid inhibiting effect on D-HBV DNA, but this effect didn't last long, and the serum level of D-HBV DNA increased again after treatment. The serum level of D-HBV DNA dropped markedly in the high- and medium-dose Mallotus apelta-treated groups on the 14th and 21st day. Low-dose Mallotus apelta revealed no obvious inhibiting effect on D-HBV. After treatment, the inhibiting effect in the root of Mallotus apelta-treated group continued as compared with that in the untreated group. The histopathological changes of liver tissues showed that the inflammation in the high-dose root of Mallotus apelta-treated group was weakened as compared with that in the lamivudine-treated group.


The root of Mallotus apelta has therapeutic effect on D-HBV. It can restrain the duplication of D-HBV in vivo. Although this effect is weaker than that of lamivudine, it continues longer. Thus this herbal medicine is an effective, safe and economical drug for hepatitis B.

Key words: Drugs, Chinese herbal, Root of Mallotus apelta (Lour.) Muell.-Arg., Duck hepatitis B virus, Quantitative real-time PCR, Animal experimentation

CLC Number: 

  • R512.62

Figure 1

Electrophoregram of PCR products of D-HBVA & B: Positive samples; C & D: Positive-detected samples."

Table 1

Copy number of D-HBV DNA in serum of nestling ducks before, during and after treatment in different groups(ヌ±S, copy/ml)"

Group n Copy number of D-HBV DNA
Pre-treatment Treatment for 7 days Treatment for 14 days Treatment for 21 days 7 days after treatment
Untreated 8 (1.95±0.95)×108 (1.73±0.74)×108 (1.47±0.71)×108 (6.64±0.77)×108 (1.45±0.75)×108*
High-dose M. 8 (1.92±1.13)×108 (1.80±1.09)×108▲▲ (6.69±2.84)×107* (4.97±4.83)×107*△△ (4.02±3.53)×107*
Medium-dose M. 8 (1.87±0.90)×108 (1.54±0.69)×108▲▲ (7.48±4.81)×107△▲▲ (5.66±4.14)×107*△△ (4.87±4.27)×107*
Low-dose M. 8 (1.73±0.66)×108 (1.52±0.61)×108▲▲ (1.51±0.61)×108▲▲ (1.36±0.47)×108▲▲ (1.28±0.65)×108*
Lamivudine- 8 (1.68±1.17)×108 (1.14±0.57)×107*△△ (6.35±8.79)×106*△△ (3.77±3.43)×106*△△ (6.82±6.39)×107

Figure 2

Histopathological changes of liver tissue in nestling ducks with D-HBV infection before and after treatment (HE staining, ×400)A: Pre-treatment; B: Lamivudine-treated group after treatment; C: High-dose Mallotus apelta-treated group after treatment."

Figure 3

Histopathological changes of liver tissue in nestling ducks with D-HBV infection beforeand after treatment (aceto-orcein staining, ×400)A: Pre-treatment; B: Lamivudine-treated group after treatment; C: High-dose Mallotus apelta-treated group after treatment."

[1] Lavanchy D . Hepatitis B virus epidemiology, disease burden, treatment, current and emerging prevention and control measures: a review[J]. J Viral Hepat, 2004,11(2):97-107
[2] 杨书兰, 张奉学, 朱宇同 , 等. 用PCR法筛选先天感染鸭乙型肝炎模型及与后天感染模型的比较[J]. 广州中医药大学学报, 2001,18(3):256-259
doi: 10.3969/j.issn.1007-3213.2001.03.018
[3] 邓学龙, 朱宇同, 方宏勋 , 等. 广州地区3个鸭种1日龄雏鸭垂直感染鸭乙型肝炎病毒调查[J]. 广州中医药大学学报, 1997,14(4):274-276
[4] 邓学龙, 朱宇同, 郭兴伯 , 等. 先天和后天感染鸭乙型肝炎病毒的广州麻鸭外周血中病毒血症的动态比较及应用[J]. 广州中医药大学学报, 1999,16(1):56-60
doi: 10.3969/j.issn.1007-3213.1999.01.017
[5] 邹文, 杨旭, 杨立平 , 等. SYBR实时定量聚合酶链反应检测鸭乙型肝炎病毒的研究[J]. 中华肝脏病杂志, 2004,12(7):444
doi: 10.3760/j.issn:1007-3418.2004.07.026
[6] 赵进军, 吕志平, 张绪富 . 白背叶根对肝纤维化大鼠的实验研究[J]. 现代诊断与治疗, 2002,13(5):257-259
doi: 10.3969/j.issn.1001-8174.2002.05.001
[1] Shu-yi Chen, Qun-wei Chen, Liu-mei Shou, Hong Pan, Shan-ming Ruan, Zhe-hao Liang, Qi-jin Shu. Stevens-Johnson syndrome/toxic epidermal necrolysis successfully treated with Chinese herbal medicine Pi-Yan-Ning: A case report. Journal of Integrative Medicine, 2021, 19(6): 555-560.
[2] Xin Yin, Shu-bin Cai, Lan-ting Tao, Lu-ming Chen, Zhong-de Zhang, Su-hong Xiao, Arthur Yin Fan, Xu Zou. Recovery of a patient with severe COVID-19 by acupuncture and Chinese herbal medicine adjuvant to standard care. Journal of Integrative Medicine, 2021, 19(5): 460-466.
[3] Qing-lan Wang, Yan-yan Tao, Hong-dong Xie, Cheng-hai Liu, Ping Liu. Fuzheng Huayu recipe, a traditional Chinese compound herbal medicine, attenuates renal interstitial fibrosis via targeting the miR-21/PTEN/AKT axis. Journal of Integrative Medicine, 2020, 18(6): 505-513.
[4] Ming-sheng Lyu, De-ying Li, Shao-zhong Zhou, Cheng-jun Ban, Jun Yan. Adult-onset Still's disease successfully treated with Chinese herbal medicine: A case report with 15-month follow-up. Journal of Integrative Medicine, 2020, 18(6): 530-534.
[5] Arthur Yin Fan, Sherman Gu, Sarah Faggert Alemi, Research Group for Evidence-based Chinese Medicine. Chinese herbal medicine for COVID-19: Current evidence with systematic review and meta-analysis. Journal of Integrative Medicine, 2020, 18(5): 385-394.
[6] Bin Lan, Jin-wen Ge, Shao-wu Cheng, Xi-long Zheng, Jun Liao, Chao He, Zheng-qing Rao, Guo-zuo Wang. Extract of Naotaifang, a compound Chinese herbal medicine, protects neuron ferroptosis induced by acute cerebral ischemia in rats. Journal of Integrative Medicine, 2020, 18(4): 344-350.
[7] Deng-hai Zhang, Kun-lun Wu, Xue Zhang, Sheng-qiong Deng, Bin Peng. In silico screening of Chinese herbal medicines with the potential to directly inhibit 2019 novel coronavirus. Journal of Integrative Medicine, 2020, 18(2): 152-158.
[8] Carola Analía Torres, Cristina Marisel Pérez Zamora, María Beatriz Nuñez, Ana María Gonzalez. In vitro antioxidant, antilipoxygenase and antimicrobial activities of extracts from seven climbing plants belonging to the Bignoniaceae. Journal of Integrative Medicine, 2018, 16(4): 255-262.
[9] Shui-jie Shen, Yong-hong Zhang, Xiao-xia Gu, Shui-ju Jiang, Ling-jun Xu. Yangfei Kongliu Formula, a compound Chinese herbal medicine, combined with cisplatin, inhibits growth of lung cancer cells through transforming growth factor-β1 signaling pathway. Journal of Integrative Medicine, 2017, 15(3): 242-251.
[10] Liliana Zazueta-Beltrán, Lorena Medina-Aymerich, Nadia Estela Díaz-Triste, Aracely Evangelina Chávez-Piña, Gilberto Castañeda-Hernández, Leticia Cruz-Antonio. Evidence against the participation of a pharmacokinetic interaction in the protective effect of single-dose curcumin against gastrointestinal damage induced by indomethacin in rats. Journal of Integrative Medicine, 2017, 15(2): 151-157.
[11] Tom Fleischer, Tung-Ti Chang, Hung-Rong Yen. Post-hematopoietic stem cell transplantation in patients with hematologic disorders: Chinese herbal medicine for an unmet need. Journal of Integrative Medicine, 2016, 14(5): 322-335.
[12] JiaXia, Li-qunHe, XiaoSu. Interventional mechanisms of herbs or herbal extracts on renal interstitial fibrosis. Journal of Integrative Medicine, 2016, 14(3): 165-173.
[13] Mojtaba Heydari, Mohammad Hashem Hashempur, Mohammad Hosein Ayati, Detlev Quintern, Majid Nimrouzi, Seyed Hamdollah Mosavat. The use of Chinese herbal drugs in Islamic medicine. Journal of Integrative Medicine, 2015, 13(6): 363-367.
[14] Wei Chiang Chan Eric, Kuin Wong Siu. Phytochemistry and pharmacology of ornamental gingers, Hedychium coronarium and Alpinia purpurata: A review. Journal of Integrative Medicine, 2015, 13(6): 368-379.
[15] Xiao-ping Huang, Huang Ding, Jin-dong Lu, Ying-hong Tang, Bing-xiang Deng, Chang-qing Deng . Autophagy in cerebral ischemia and the effects of traditional Chinese medicine. Journal of Integrative Medicine, 2015, 13(5): 289-296.
Full text



[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239