丹酚酸B对兔缺血再灌注心脏内皮细胞功能和血小板活化的影响

doi:10.3736/jcim20081208

Abstract

Objective:

To investigate the effects of salvianolic acid B (SA-B) on cardiovascular endothelial cell function and platelet activation during myocardial ischemia-reperfusion in rabbits.

Methods:

A total of 24 New Zealand white rabbits were randomly divided into sham-operated group, ischemia-reperfusion group (untreated group) and SA-B group. The hearts of rabbits in untreated group and SA-B group underwent half an hour of left anterior descending coronary artery (LADCA) occlusion via ligation technology, which was followed by 4 hours of reperfusion to prepared ischemia-reperfusion injury model in vivo. For sham-operated group, the animals were not subjected to occlusion of LADCA. In SA-B treatment group the rabbits were intravenously administered SA-B immediately after LADCA occlusion, and the other two groups were given normal saline in the same way instead of SA-B. The jugular vein bloods of animals were collected before LADCA ligation, half an hour after ligation and after 1-, 4-hour reperfusion, respectively. The content of plasma nitric oxide (NO) was determined by nitrate reductase process. Radioimmunoassay was applied to detect the endothelin (ET) content in plasma and the count of alpha-granule membrane protein-140 (GMP-140) on platelet surface to identify the activation of the platelet.
Results:

No significant difference was observed before and after sham LADCA occlusion in sham-operated group in the contents of NO and ET in plasma (P>0.05), neither was the count of GMP-140 on platelet surface (P>0.05). The content of NO in plasma detected 0.5 h after LADCA occlusion was significantly decreased in untreated group compared with the sham-operated group at the corresponding time, and they were also much lower than that before LADCA occlusion in the sham-operated group (P<0.05). The plasma content of NO in untreated group showed a progressive decrease in response to the myocardial reperfusion. However, the content of ET in plasma and the count of GMP-140 on platelet surface were remarkably increased after myocardial ischemia as compared with those before LADCA ligation and those detected in sham-operated group (P<0.05). The content of ET and the count of GMP-140 in the untreated group were further increased corresponding to the aggressive reperfusion. The content of NO was significantly increased while the content of ET and the count of GMP-140 were both significantly decreased in SA-B group as compared with untreated group after 1- and 4-hour myocardial reperfusion, respectively (P<0.01).

Conclusion:

The results show that endothelial dysfunction and platelet activation occur during ischemia-reperfusion in rabbit hearts in vivo and SA-B protects cardiovascular endothelium cells against ischemia-reperfusion injury and inhibits the activation of platelet during myocardial ischemia and reperfusion.

Key words: salvianolic acid B, myocardical reperfusion injury, nitric oxide, endothelin, platelet alpha-granule membrane protein-140, rabbits

CLC Number:

R285.5

Fu-guo Yang, An-yong Zhang, Zuo-yuan Chen, Zhe-xun Chen, Ge-xin Liu, Guo-xiong Dong. Effects of salvianolic acid B on cardiovascular endothelial cells and platelet activation in a rabbit model of ischemia-reperfusion. Journal of Chinese Integrative Medicine, 2008, 6(12): 1250-1254.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20081208

http://www.jcimjournal.com/EN/Y2008/V6/I12/1250

TrendMD

Figures/Tables 3

Table 1

Table 2

Table 3

References 16

[1]	Hoffman JW Jr, Gilbert TB, Poston RS , et al. Myocardial reperfusion injury: etiology, mechanisms, and therapies[J]. J Extra Corpor Technol, 2004,36(4):391-411 pmid: 15679285
[2]	Xu J, Fan WH . Effect of salvianolate on migration of human vascular endothelial cells[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(3):211-214
	徐杰, 范维琥 . 丹参多酚酸盐对人血管内皮细胞迁移的影响[J]. 中西医结合学报, 2003,1(3):211-214
[3]	Liu W, Zhang Y, Zuo J . Protective effects and mechanism of salvianolic acid B(SAB) on PC12 glucose deprivation injury[J]. Shanghai Zhong Yi Yao Za Zhi, 2006,40(10):66-68 doi: 10.3969/j.issn.1007-1334.2006.10.029
	刘雯, 张艳, 左伋 . 丹酚酸B对PC12细胞缺糖损伤的保护作用及机制[J]. 上海中医药杂志, 2006,40(10):66-68 doi: 10.3969/j.issn.1007-1334.2006.10.029
[4]	Wu J, Xu L, Xu L . Protective effect of salvianolic acid B against anoxia-reoxygenation injury in isolated rabbit hearts[J]. Zhongguo Yao Li Xue Yu Du Li Xue Za Zhi, 2007,21(3):212-216
	武骏, 徐立, 许立 . 丹酚酸B对兔离体心脏缺氧再复氧损伤的保护作用[J]. 中国药理学与毒理学杂志, 2007,21(3):212-216
[5]	Wang XN, Hu YY, Liu CH , et al. Effects of salvianolic acid B on expressions of TGF-β1 and its receptors in liver of rats with dimethylnitrosamine-induced hepatic fibrosis[J]. Zhong Xi Yi Jie He Xue Bao, 2005,3(4):286-289
	王晓柠, 胡义扬, 刘成海 , 等. 丹酚酸B盐对二甲基亚硝胺诱导的肝纤维化大鼠肝脏转化生长因子β1及其受体蛋白表达的影响[J]. 中西医结合学报, 2005,3(4):286-289
[6]	Yao Z, Chen YF, Feng JZ , et al. Effect of experimental ischemic preconditioning on reducing myocyte apoptosis in rabbits[J]. Zhongguo Dong Mai Ying Hua Za Zhi, 1999,7(1):20-23 doi: 10.1361/105497199770335938
	姚震, 陈颜芳, 冯建章 , 等. 实验性家兔缺血预适应减轻心肌细胞凋亡[J]. 中国动脉硬化杂志, 1999,7(1):20-23 doi: 10.1361/105497199770335938
[7]	Wu GX, Ruan CG . Comparison of four different activation-dependent proteins on the platelet surface in detecting platelet activation[J]. Suzhou Yi Xue Yuan Xue Bao, 1993,13(4):263-265
	吴国新, 阮长耿 . 检测体外血小板活化程度时4种血小板表面活化标志蛋白的比较[J]. 苏州医学院学报, 1993,13(4):263-265
[8]	Moens AL, Claeys MJ, Timmermans JP , et al. Myocardial ischemia/reperfusion-injury, a clinical view on a complex pathophysiological process[J]. Int J Cardiol, 2005,100(2):179-190 doi: 10.1016/j.ijcard.2004.04.013
[9]	Doggrell SA . The endothelin system and its role in acute myocardial infarction[J]. Expert Opin Ther Targets, 2004,8(3):191-201 doi: 10.1517/14728222.8.3.191 pmid: 15161426
[10]	Hou XL, Li BL, Zhao L , et al. Effects of Xuefu Zhuyu Capsule on endothelin-1 release in myocardium and vascular endothelium and nitric oxide/nitric oxide synthase system of swines after acute myocardial infarction and reperfusion[J]. Zhong Xi Yi Jie He Xue Bao, 2008,6(4):381-386
	侯宵雷, 李白翎, 赵雷 , 等. 血府逐瘀胶囊对急性心肌梗死再灌注后心肌及内皮素-1 一氧化氮/一氧化氮合酶体系影响的实验研究[J]. 中西医结合学报, 2008,6(4):381-386
[11]	Kaneda H, Taguchi J, Kuwada Y , et al. Coronary artery spasm and the polymorphisms of the endothelial nitric oxide synthase gene[J]. Circ J, 2006,70(4):409-413 doi: 10.1253/circj.70.409 pmid: 16565556
[12]	Elesber AA, Solomon H, Lennon RJ , et al. Coronary endothelial dysfunction is associated with erectile dysfunction and elevated asymmetric dimethylarginine in patients with early atherosclerosis[J]. Eur Heart J, 2006,27(7):824-831 doi: 10.1093/eurheartj/ehi749
[13]	Yang YJ, Zhao JL, You SJ , et al. Different effects of tirofiban and aspirin plus clopidogrel on myocardial no-reflow in a mini-swine model of acute myocardial infarction and reperfusion[J]. Heart, 2006,92(8):1131-1137 doi: 10.1136/hrt.2005.077164
[14]	Alfayoumi F, Srinivasan V, Geller M , et al. The no-reflow phenomenon: epidemiology, pathophysiology, and therapeutic approach[J]. Rev Cardiovasc Med, 2005,6(2):72-83 pmid: 15976730
[15]	Zhou Y, Gu J, Xu LM . Effect and mechanism of salvianolic acid B in attenuating elevated portal pressure in a rat model of portal hypertension induced by endothelin-1[J]. Zhong Xi Yi Jie He Xue Bao, 2007,5(1):61-64
	周扬, 顾杰, 徐列明 . 丹参酚酸B盐抑制内皮素-1所致大鼠门静脉压力升高的药效及其作用机制[J]. 中西医结合学报, 2007,5(1):61-64
[16]	Liu GS, Xu GF . Research advance in antioxidation of salvianolic acid[J]. Shi Pin Yu Yao Pin, 2007,9(1):69-71 doi: 10.3969/j.issn.1672-979X.2007.01.023
	刘根生, 徐贵发 . 丹酚酸的抗氧化作用[J]. 食品与药品, 2007,9(1):69-71 doi: 10.3969/j.issn.1672-979X.2007.01.023

Related Articles 15

[1]	Swatantra Kumar, Vimal K Maurya, Debadatta Nayak, Anil Khurana, Raj K Manchanda, Srinivasulu Gadugu, Madan LB Bhatt, Shailendra K Saxena. Calcarea carbonica treatment rescues lipopolysaccharide-induced inflammatory response in human mononuclear cells via downregulation of inducible cyclooxygenase pathway. Journal of Integrative Medicine, 2020, 18(5): 441-449.
[2]	The Su Moe, Htet Htet Win, Thin Thin Hlaing, War War Lwin, Zaw Min Htet, Khin Mar Mya . Evaluation of in vitro antioxidant, antiglycation and antimicrobial potential of indigenous Myanmar medicinal plants. Journal of Integrative Medicine, 2018, 16(5): 358-366.
[3]	Byung Hyuk Han, Yun Jung Lee, Jung Joo Yoon, Eun Sik Choi, Seung Namgung, Xian Jun Jin, Da Hye Jeong, Dae Gill Kang, Ho Sub Lee. Hwangryunhaedoktang exerts anti-inflammation on LPS-induced NO production by suppressing MAPK and NF-κB activation in RAW264.7 macrophages. Journal of Integrative Medicine, 2017, 15(4): 326-336.
[4]	Omowumi Titilola Kayode, Musa Toyin Yakubu. Parquetina nigrescens leaves: chemical profile and influence on the physical and biochemical indices of sexual activity of male Wistar rats. Journal of Integrative Medicine, 2017, 15(1): 64-76.
[5]	Rajendra Karki, Cheol-Ho Park, Dong-Wook Kim. Extract of buckwheat sprouts scavenges oxidation and inhibits pro-inflammatory mediators in lipopolysaccharide-stimulated macrophages (RAW264.7). Journal of Chinese Integrative Medicine, 2013, 11(4): 246-252.
[6]	Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[7]	Ju-yong Wang, Jing Guo, Chang-sheng Dong, Zhan Zheng, Qing Wang. Effects of Feiyanning Decoction, a compound traditional Chinese medicine, on iNOS and COX-2 expressions induced by tumor necrosis factor-α in lung adenocarcinoma cell line. Journal of Chinese Integrative Medicine, 2012, 10(6): 695-700.
[8]	Ya-fang Song, Zhi-gang Lv, Lie-ruing Xu. Salvianolic acid B inhibits ERK signal transduction pathway activated by transforming growth factor-β1 in rat hepatic stellate cells. Journal of Chinese Integrative Medicine, 2012, 10(4): 454-461.
[9]	Tapas Kumar Sur, Biswajit Auddy, Dipankar Bhattacharyya. Effects of Indian herbal formulation Body Revival on human platelet aggregation and myocardial ischemia in rats. Journal of Chinese Integrative Medicine, 2011, 9(7): 746-751.
[10]	Bamidele Victor Owoyele, Omosunkanmi Toyin Adenekan, Ayodele Olufemi Soladoye. Effects of honey on inflammation and nitric oxide production in Wistar rats. Journal of Chinese Integrative Medicine, 2011, 9(4): 447-452.
[11]	Zhao Li, Liang Xiao-chun, Zhang Hong, Wang Pu-yan, Sun Qing, Huang Wen-zhi, Li Bo-wu. Effects of Chinese herbal medicine Jinmaitong-medicated serum on inducible nitric oxide synthase and NADPH oxidase p22-phox subunit of rat Schwann cells cultured in high-glucose medium. Journal of Chinese Integrative Medicine, 2011, 9(3): 299-305.
[12]	Gollapalle L. Viswanatha, Hanumanthappa Shylaja, K. Sadashiva Sandeep Rao, Yathiraj Ashwini, V. Ramaiah Santhosh Kumar, C. Gangadharaiah Mohan, Venkate Gowda Sunil, M. Venkateshappa Sarvesh Kumar, Subbanna Rajesh. Amelioration of immobilization stress-induced biochemical and behavioral alterations and mitochondrial dysfunction by naringin in mice: Possible mechanism of nitric oxide modulation. Journal of Chinese Integrative Medicine, 2011, 9(11): 1254-1263.
[13]	Qi-qi Mao , Xu Sun, Dong-ni Qiu, Xiao-dong Fu, Yi Liu, Wen-jian Wang. Effects of Fufang Jiangzhi No.3 on cholesterol-bile acid metabolism in rabbits with hypercholesterolemia. Journal of Chinese Integrative Medicine, 2010, 8(5): 453-457.
[14]	Hui Qiang, Kun-zheng Wang, Chen Zhang, Zhi-bin Shi, Li-hong Fan. Panax notoginseng saponins protect rabbit bone marrow stromal cells from hydrogen peroxide-induced apoptosis. Journal of Chinese Integrative Medicine, 2010, 8(2): 131-137.
[15]	Bin Cheng, Xin Wang, Lei Wang, Kai Cao, Lei Duan, Feng-tao Li. Changes of mitochondrial apoptosis in spinal cord ischemia-reperfusion injury and the effects of Herba Erigerontis Breviscapi Injection preconditioning intervention in rabbits. Journal of Chinese Integrative Medicine, 2009, 7(9): 842-847.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2]	Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3]	Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4]	Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5]	. Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6]	Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7]	Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8]	Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9]	Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10]	Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228

Group	n	NO content
Group	n	Before LADCA ligation	0.5 h ischemia	1 h reperfusion	4 h reperfusion
Sham-operated	8	98.35±14.75	97.52±12.49	92.08±10.42	94.58±11.78
Untreated	8	95.64±17.56	78.43±18.73*^△	39.30±9.98**^△△	21.73±7.44**^△△
SA-B	8	92.62±15.71	76.33±16.58*	73.53±12.77*^▲▲	72.71±13.58*^▲▲

Group	n	ET content
Group	n	Before LADCA ligation	0.5 h ischemia	1 h reperfusion	4 h reperfusion
Sham-operated	8	96.21±22.26	97.71±25.45	101.15±31.13	105.08±36.13
Untreated	8	98.86±20.68	137.91±29.81*^△	195.66±30.25**^△△	246.91±43.37**^△△
SA-B	8	99.98±19.37	133.28±36.31*	131.16±21.03*^▲▲	125.78±15.15*^▲▲

Group	n	GMP-140 count
Group	n	Before LADCA ligation	0.5 h ischemia	1 h reperfusion	4 h reperfusion
Sham-operated	8	730.63±119.60	745.51±126.31	806.75±219.78	844.25±257.05
Untreated	8	735.88±122.31	1 194.88±405.25*^△	1 652.01±280.49**^△△	1 958.25±455.18**^△△
SA-B	8	753.13±110.95	1 165.63±524.39*	1 145.75±237.23*^▲▲	1 092.25±175.75*^▲▲

Effects of salvianolic acid B on cardiovascular endothelial cells and platelet activation in a rabbit model of ischemia-reperfusion

RichHTML

PDF (PC)

Cited

Like