Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (9): 911-914.

• Original Experimental Research • Previous Articles     Next Articles

Effects of ginsenoside Rg1 on expression of insulin-like growth factor-1 in brain of rats with brain contusion

Wen-kai Li1, Wen-bin Ran2, Ying-wen Su1, Fei-jun Huang1()   

  1. 1. Department of Forensic Pathology, West China College of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province 610041, China
    2. Laboratory of Preclinical Medicine, West China College of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province 610041, China
  • Received:2008-01-04 Online:2008-09-20 Published:2008-09-15

Objective: To study the effects of ginsenoside Rg1 on the expression of insulin-like growth factor-1 (IGF-1) in the brain of rats after the experimental brain contusion.
Methods: A total of twenty-six Wistar rats were randomly divided into normal control group (n=2), untreated group (n=8) and ginsenoside Rg1 group (n=16). Brain injuries were induced in rats by a mechanical striking device. The brain tissues were extracted at different times after brain injury (6th hour, 12th hour, 2nd day, 6th day), then the expression of IGF-1 in brain tissue was examined by immunohistochemical method.
Results: In comparison with the normal control group, the expression of IGF-1 in the brain tissues was increased in the untreated group after the brain contusion ( P<0.05). The expression of IGF-1 in brain tissues in ginsenoside Rg1 group was significantly increased as compared with the untreated group ( P<0.05).
Conclusion: Ginsenoside Rg1 enhances the recovery of the contused brain through increasing the expression of IGF-1.

Key words: brain contusion, insulin-like growth factor-1, ginsenoside Rgl, rats

CLC Number: 

  • R285.5

Table 1

Counts of IGF-1 positive cells in brain tissues of rats in different groups (x±s)"

Group n Counts of IGF-1 positive cells
6 hours 12 hours 2 days 6 days
Normal control 2 432±57
Untreated 2 504±36* 904±68 1 269±75 1 619±150
Ginsenoside Rg1 4 571±24 1 060±136 1 449±143 2 587±121

Figure 1

Expression of IGF-1 in brain tissues of rats in untreated group at different times after the brain contusion (SP staining, ×400) A: 6 hours after brain contusion; B: 2 days after brain contusion."

Figure 2

Expression of IGF-1 in brain tissues of rats in ginsenoside Rg1 group at different times after the brain contusion ( SP staining, ×400) A: 6 hours after brain contusion; B: 2 days after brain contusion."

[1] Cui RT, Pu CQ, Liu JX , et al. Effects of ginsenoside Rg1 on the proliferation of neural stem cells in rats with focal cerebral ischemia[J]. Jie Fang Jun Yi Xue Za Zhi, 2007,32(8):842-845
崔荣太, 蒲传强, 刘洁晓 , 等. 人参皂甙Rg1对大鼠局灶性脑缺血脑组织神经干细胞增殖的影响[J]. 解放军医学杂志, 2007,32(8):842-845
[2] Cui RT, Pu CQ, Wang PF , et al. Effect of ginsenoside Rg1 on the expression of neuron specific enolase following focal cerebral ischemia in rats and its significance[J]. Lin Chuang Shen Jing Bing Xue Za Zhi, 2007,20(2):122-124
doi: 10.3969/j.issn.1004-1648.2007.02.014
崔荣太, 蒲传强, 王培福 , 等. 人参皂甙Rg1对局灶性脑缺血大鼠脑组织神经元特异性烯醇化酶表达的影响及其意义[J]. 临床神经病学杂志, 2007,20(2):122-124
doi: 10.3969/j.issn.1004-1648.2007.02.014
[3] Cui RT, Pu CQ, Wang PF , et al. Effect of ginsenoside Rg1 on the expression of GFAP following cerebral ischemia in rats[J]. Zhongguo Zhong Yi Yao Ke Ji, 2007,14(2):86-88
doi: 10.3969/j.issn.1005-7072.2007.02.011
崔荣太, 蒲传强, 王培福 , 等. 人参皂甙Rg1 对局灶性脑缺血大鼠脑组织GFAP 表达的影响[J]. 中国中医药科技, 2007,14(2):86-88
doi: 10.3969/j.issn.1005-7072.2007.02.011
[4] Liu ZC, Wang MY . Insulin-like growth factor-1 and cerebral ischemia[J]. Guo Wai Yi Xue Nao Xue Guan Ji Bing Fen Ce, 2001,9(3):162-164
doi: 10.3760/cma.j.issn.1673-4165.2001.03.007
刘宗超, 王慕一 . 胰岛素样生长因子1与脑缺血[J]. 国外医学脑血管疾病分册, 2001,9(3):162-164
doi: 10.3760/cma.j.issn.1673-4165.2001.03.007
[5] Kazanis I, Bozas E, Philippidis H , et al. Neuroprotective effects of insulin-like growth factor-I(IGF-I) following a penetrating brain injury in rats[J]. Brain Res, 2003,991(1-2):34-45
doi: 10.1016/S0006-8993(03)03525-X
[6] Feeney DM, Boyeson MG, Linn RT , et al. Responses to cortical injury: I. Methodology and local effects of contusions in the rat[J]. Brain Res, 1981,211(1):67-77
doi: 10.1016/0006-8993(81)90067-6 pmid: 7225844
[7] Cohick WS, Clemmons DR .The insulin-like growth factors[J].Annu Rev Physiol, 1993, 55: 131-153
doi: 10.1146/annurev.ph.55.030193.001023
[8] D'Ercole AJ, Ye P, O'Kusky JR .Mutant mouse models of insulin-like growth factor actions in the central nervous system[J]. Neuropeptides, 2002,36(2-3):209-220
doi: 10.1054/npep.2002.0893 pmid: 12359511
[9] Sherrard RM . Insulin-like growth factor 1 induces climbing fibre re-innervation of the rat cerebellum[J]. Neuroreport, 1997,8(15):3225-3228
doi: 10.1097/00001756-199710200-00008 pmid: 9351647
[10] Sandberg Nordqvist AC, von Holst H, Holmin S , et al. Increase of insulin-like growth factor(IGF)-1, IGF binding protein-2 and -4 mRNAs following cerebral contusion[J]. Brain Res Mol Brain Res, 1996,38(2):285-293
doi: 10.1016/0169-328X(95)00346-T
[11] Walter HJ, Berry M, Hill DJ , et al. Spatial and temporal changes in the insulin-like growth factor(IGF) axis indicate autocrine/paracrine actions of IGF-I within wounds of the rat brain[J]. Endocrinology, 1997,138(7):3024-3034
doi: 10.1210/endo.138.7.5284
[12] Shen SJ, Zhang SQ, Chen JF , et al. Expression of IGF-I mRNA in focal cerebral ischemia injury in rats[J]. Zhong Feng Yu Shen Jing Ji Bing Za Zhi, 2003,20(4):327-329
沈顺姬, 张淑琴, 陈嘉峰 , 等. 大鼠局灶性脑缺血损伤中IGF-I mRNA表达[J]. 中风与神经疾病杂志, 2003,20(4):327-329
[13] Liu GY, Zhang D, Du XM , et al. Experiment promotion neurons regeneration and expression of GAP-43 proteins and IGF-1 proteins after focal cerebral ischemia reperfusion injury in rats[J]. Zhong Feng Yu Shen Jing Ji Bing Za Zhi, 2007,24(4):398-402
刘广义, 张笛, 杜秀民 , 等. 大鼠局灶性脑缺血再灌注损伤后GAP-43及IGF-1促进神经元再生表达的实验研究[J]. 中风与神经疾病杂志, 2007,24(4):398-402
[14] Chu ZH, Fei SZ, Ma LS , et al. Expression of IGF-1 in rat brain and its effect on apoptosis after intracerebral hemorrhage[J]. Zhonghua Shen Jing Yi Xue Za Zhi, 2006,5(11):1102-1104
doi: 10.3760/cma.j.issn.1671-8925.2006.11.007
储照虎, 费世早, 马领松 , 等. 脑出血大鼠脑组织IGF-1的表达及其对细胞凋亡的影响[J]. 中华神经医学杂志, 2006,5(11):1102-1104
doi: 10.3760/cma.j.issn.1671-8925.2006.11.007
[15] Kang DF, Liu Y, Wang YY , et al. Relation between HIF-1α/IGF-1 expressions and the post brain injury interval in rats[J]. Fa Yi Xue Za Zhi, 2007,23(3):174-176
doi: 10.3969/j.issn.1004-5619.2007.03.005
亢登峰, 刘扬, 王英元 , 等. HIF-1α、IGF-1表达与脑挫伤时间的相关性[J]. 法医学杂志, 2007,23(3):174-176
doi: 10.3969/j.issn.1004-5619.2007.03.005
[1] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[2] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[3] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[4] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[5] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[6] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[7] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[8] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[9] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[10] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[11] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[12] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[13] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
[14] Dilpesh Jain​, Manish Kumar Bansal, Rahul Dalvi​, Aman Upganlawar​, Rahul Somani​. Protective effect of diosmin against diabetic neuropathy in experimental rats. Journal of Integrative Medicine, 2014, 12(1): 35-41.
[15] Manjusha Choudhary​, Vipin Kumar​, Surender Singh​. Gastric antisecretory and cytoprotective effects of hydroalcoholic extracts of Plumeria alba Linn. leaves in rats. Journal of Integrative Medicine, 2014, 12(1): 42-51.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[4] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[5] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[6] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[7] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[8] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[9] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022