小檗碱对大鼠骨髓源性破骨细胞的分化及骨吸收功能的影响

doi:10.3736/jcim20090408

Abstract

Objective: To observe the effects of berberine on osteoclastic differentiation and bone resorption action in vitro, and to investigate the cellular mechanism of its inhibitory effects on bone resorption.
Methods: The multinucleated osteoclasts (MNCs) were derived by 1,25-dihydroxyvitamin D3 and dexamethasone from bone marrow cells in the coculture system with primary osteoblastic cells. The tartrate-resistant acid phosphatase (TRAP) staining and image analysis of bone resorption pit on dental slices were used to identify osteoclast. The activity of TRAP was measured by p-nitrophenyl sodium phosphate assay. The bone resorption pit area on the bone slices formed by osteoclasts was measured by computer image processing.
Results: At the concentrations of 0.1, 1 and 10 μmol/L, berberine dose-dependently suppressed the formation of TRAP-positive multinucleated cells, the TRAP activity and the osteoclastic bone resorption. The strongest inhibitory effect was exhibited at the concentration of 10 μmol/L, with the inhibiting rate of 60.45%, 42.12% and 72.69% respectively.
Conclusion: Berberine can decrease bone loss through inhibition of osteoclast formation, differentiation and bone resorption.

Key words: berberine, osteoporosis, osteoclasts, bone resorption

WEI Peng, JIAO Lei, QIN Lu-ping, YAN Fei, HAN Ting, ZHANG Qiao-yan. Effects of berberine on differentiation and bone resorption of osteoclasts derived from rat bone marrow cells. Journal of Chinese Integrative Medicine, 2009, 7(4): 342-348.

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References 24

[1]	Dai RC, Zhang L, Liao EY . Progress in diagnosis and treatment of osteoporosis[J]. Zhongguo Yi Kan, 2008,43(4):4-6
	戴如春, 张丽, 廖二元 . 骨质疏松的诊治进展[J]. 中国医刊, 2008,43(4):4-6
[2]	Davison S, Davis SR . Hormone replacement therapy: current controversies[J]. Clin Endocrinol (Oxf), 2003,58(3):249-261 doi: 10.1046/j.1365-2265.2003.01774.x
[3]	Hu JP, Takahashi N, Yamada T . Coptidis rhizoma inhibits growth and proteases of oral bacteria[J]. Oral Dis, 2000,6(5):297-302
[4]	Anis KV, Rajeshkumar NV, Kuttan R . Inhibition of chemical carcinogenesis by berberine in rats and mice[J]. Pharm Pharmacol, 2001,53(5):763-768 doi: 10.1211/0022357011775901
[5]	Hwang JM, Kuo HC, Tseng TH, Liu JY, Chu CY . Berberine induces apoptosis through a mitochondria/caspases pathway in human hepatoma cells[J]. Arch Toxicol, 2006,80(2):62-73 doi: 10.1007/s00204-005-0014-8
[6]	Kuo CL, Chou CC, Yung BY . Berberine complexes with DNA in the berberine-induced apoptosis in human leukemic HL-60 cells. Cancer Lett[J]. Cancer Lett, 1995,93(2):193-200 doi: 10.1016/0304-3835(95)03809-B
[7]	Mantena SK, Sharma SD, Katiyar SK . Berberine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependent apoptosis in human prostate carcinoma cells[J]. Mol Cancer Ther, 2006,5(2):296-308 doi: 10.1158/1535-7163.MCT-05-0448
[8]	Li H, Miyahara T, Tezuka Y, Namba T, Nemoto N, Tonami S, Seto H, Tada T, Kadota S . The effect of Kampo formulae on bone resorption in vitro and in vivo. Ⅰ. Active constituents of Tsu-kan-gan[J]. Biol Pharm Bull, 1998,21(12):1322-1326 doi: 10.1248/bpb.21.1322
[9]	Li H, Miyahara T, Tezuka Y, Namba T, Suzuki T, Dowaki R, Watanabe M, Nemoto N, Tonami S, Seto H, Kadota S . The effect of kampo formulae on bone resorption in vitro and in vivo. Ⅱ. Detailed study of berberine[J]. Biol Pharm Bull, 1999,22(4):391-396 doi: 10.1248/bpb.22.391
[10]	Qin L, Han T, Zhang Q, Cao D, Nian H, Rahman K, Zheng H . Antiosteoporotic chemical constituents from Er-Xian Decoction, a traditional Chinese herbal formula[J]. J Ethnopharmacol, 2008,118(2):271-279 doi: 10.1016/j.jep.2008.04.009
[11]	Nian H, Xu LL, Ma MH, Zheng HC, Zhang QY, Qin LP . Effects of berberine from Cortex Phellodendri Chinensis on osteoporosis in ovariectomized rats[J]. Yao Xue Fu Wu Yu Yan Jiu, 2007,7(1):41-44
	年华, 徐玲玲, 马明华, 郑汉臣, 张巧艳, 秦路平 . 黄柏小檗碱对去卵巢大鼠骨质疏松症的作用[J]. 药学服务与研究, 2007,7(1):41-44
[12]	Hu JP, Nishishita K, Sakai E, Yoshida H, Kato Y, Tsukuba T, Okamoto K . Berberine inhibits RANKL-induced osteoclast formation and survival through suppressing the NF-kappaB and Akt pathways[J]. Eur J Pharmacol, 2008,5802(1-2):70-79
[13]	Rodan GA, Martin TJ . Role of osteoblasts in hormonal control of bone resorption — a hypojournal[J]. Calcif Tissue Int, 1981,33(4):349-351 doi: 10.1007/BF02409454
[14]	Rodan GA, Martin TJ . Therapeutic approaches to bone diseases[J]. Science, 2000,289(5484):1508-1514 doi: 10.1126/science.289.5484.1508
[15]	Teitelbaum SL . Bone resorption by osteoclasts[J]. Science, 2000,289(5484):1504-1508 doi: 10.1126/science.289.5484.1504
[16]	Zhang QH, Gao HY, Wu LJ, Zhang L . Chemical constituents of Evodia rutaecarpa(Juss.) Benth[J]. Shenyang Yao Ke Da Xue Xue Bao, 2005,22(1):12-14
	张起辉, 高慧媛, 吴立军, 张磊 . 吴茱萸的化学成分[J]. 沈阳药科大学学报, 2005,22(1):12-14
[17]	Liu ZD, Zang HS, Ouyang YP . Biological study of growth pattern of newborn rat calvaria osteoblastic cell in vitro[J]. Jie Pou Xue Bao, 1995,26(2):157-160
	刘祖德, 臧鸿声, 欧阳跃平 . 新生大鼠颅骨成骨性细胞体外生长过程研究[J]. 解剖学报, 1995,26(2):157-160
[18]	Li B, Yu S . Genistein prevents bone resorption diseases by inhibiting bone resorption and stimulating bone formation[J]. Biol Pharm Bull, 2003,26(6):780-786 doi: 10.1248/bpb.26.780
[19]	Yasukawa K, Takido M, Ikekawa T, Shimada F, Takeuchi M, Nakagawa S . Relative inhibitory activity of berberine-type alkaloids against 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice[J]. Chem Pharm Bull (Tokyo), 1991,39(6):1462-1465 doi: 10.1248/cpb.39.1462
[20]	Fukuda K, Hibiya Y, Mutoh M, Koshiji M, Akao S, Fujiwara H . Inhibition by berberine of cyclooxygenase-2 transcriptional activity in human colon cancer cells[J]. J Ethnopharmacol, 1999,66(2):227-233 doi: 10.1016/S0378-8741(98)00162-7
[21]	Liu XH, Kirschenbaum A, Yao S, Levine AC . Cross-talk between the interleukin-6 and prostaglandin E2 signaling systems results in enhancement of osteoclastogenesis through effects on the osteoprotegerin/receptor activator of nuclear factor-kappaB(RANK) ligand/RANK system[J]. Endocrinology, 2005,146(4):227-233
[22]	Zhang X, Schwarz EM, Young DA, Puzas JE, Rosier RN , O'Keefe RJ.Cyclooxygenase-2 regulates mesenchymal cell differentiation into the osteoblast lineage and is critically involved in bone repair[J]. J Clin Invest, 2002,109(11):1405-1415 doi: 10.1172/JCI0215681
[23]	Ono K, Akatsu T, Murakami T, Kitamura R, Yamamoto M, Shinomiya N, Rokutanda M, Sasaki T, Amizuka N, Ozawa H, Nagata N, Kugai N . Involvement of cyclo-oxygenase-2 in osteoclast formation and bone destruction in bone metastasis of mammary carcinoma cell lines[J]. J Bone Miner Res, 2002,17(5):774-781 doi: 10.1359/jbmr.2002.17.5.774
[24]	Zhang X, Morham SG, Langenbach R, Young DA, Xing L, Boyce BF, Puzas EJ, Rosier RN , O'Keefe RJ, Schwarz EM.Evidence for a direct role of cyclo-oxygenase 2 in implant wear debris-induced osteolysis[J]. J Bone Miner Res, 2001,16(4):660-670 doi: 10.1359/jbmr.2001.16.4.660

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Effects of berberine on differentiation and bone resorption of osteoclasts derived from rat bone marrow cells

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