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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (6): 552-556.doi: 10.3736/jcim20090611

• Original Experimental Research • Previous Articles     Next Articles

Effects of Liangxue Jiedu Decoction in treating psoriasis in a mouse psoriasis model

Min-jie Gu,Shang-pu Gao, Yong-mei Li   

  1. Department of Dermatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-12-29 Accepted:2009-03-16 Online:2009-06-20 Published:2009-06-15

Objective

To study the effects of Liangxue Jiedu Decoction, a compound traditional Chinese herbal medicine with the function of blood-cooling and detoxicating, in treating psoriasis in mice and to explore its mechanism.
Methods

(1) Sixty mice were randomly divided into Liangxue Jiedu Decoction group, compound Indigo Naturalis capsule group, acitretin capsule group and normal saline group. Another 10 mice were selected as blank control. After 2-week administration, mice were sacrificed to obtain samples. After hematoxylin and eosin (HE) staining, tail scales with granular layers were calculated by an optical microscope. (2) Except for ten mice in blank group, sixty female mice were injected intraperitoneally with diethylstilbestrol once daily. After 3-day injection, mice were randomly divided into four groups and treated as above description. After 2-week treatment, all mice were injected intraperitoneally with colchicine (2 mg/kg), and sacrificed 6 h after the injection. The mitotic rate in virginal epithelium was calculated after HE staining.
Results

Compared with normal saline, Liangxue Jiedu Decoction could significantly inhibit the mitosis of mouse vaginal epithelium (P<0.01) and promote the formation of granular layers in mouse tail-scale epidermis (P<0.01).
Conclusion

The mechanism of Liangxue Jiedu Decoction in treating psoriasis may be related to promoting granular cell growth and inhibiting proliferation of epidermic cells.

Key words: Liangxue Jiedu Decoction, Psoriasis, Mitosis, Mice

Table 1

Effects of Liangxue Jiedu Decoction on formation of granular layers in mouse tail-scale epidermis (x±s, %)"

Group n Granular layer of scale
Blank 10 4.27±0.65
Normal saline 15 4.33±0.62
Acitretin capsule 15 13.53±2.53**
Compound Indigo Naturalis capsule 15 10.80±2.24**△△
Liangxue Jiedu Decoction 15 12.47±1.89**

Figure 1

Granular layers in mouse tail-scale epidermis observed by light microscopy (HE staining, ×400) A: Blank group; B: Normal saline group; C: Acitretin capsule group; D: Compound Indigo Naturalis capsule group; E: Liangxue Jiedu Decoction group."

Figure 2

Mitosis of mouse vaginal epithelium observed by light microscopy (HE staining, ×400) A: Blank group; B: Normal saline group; C: Acitretin capsule group; D: Compound Indigo Naturalis capsule group; E: Liangxue Jiedu Decoction group."

Table 2

Effects of Liangxue Jiedu Decoction on mitosis of mouse vaginal epithelium (x±s, %)"

Group n Epithelial mitosis
Blank 10 8.90±1.87**
Normal saline 15 12.60±1.55
Acitretin capsule 15 6.87±0.92**
Compound Indigo Naturalis capsule 15 7.33±1.40**
Liangxue Jiedu Decoction 15 7.47±1.25**
[1] Li YM, Ma SY, Feng GQ, He CD, Song Y . Clinical effect observation on 495 cases of psoriasis treated by regulating the liver[J]. Zhejiang Zhong Xi Yi Jie He Za Zhi, 2007,17(12):776-777
李咏梅, 马绍尧, 冯国强, 何川娣, 宋瑜 . 从肝论治银屑病495例疗效观察.[J]. 浙江中西医结合杂志, 2007,17(12):776-777
[2] Van Scott EJ, Bonder RH . Intravaginal and intrarectal screening of antimitotic drugs for topical effectiveness[J]. J Invest Dermatol, 1971,56(2):132-139
doi: 10.1111/1523-1747.ep12260707
[3] Zhang DF. Pharmacology and experimental pharma-cology of Chinese materia medica[M]. Shanghai: Shanghai Scientific and Technical Publishers,2002: 8-9
张大方 . 药理与中药药理实验[M]. 上海: 上海科学技术出版社,2002: 8-9
[4] Bonder RH, Van Scott EJ . Use of mouse vaginal and rectal epithelium to screen antimitotic effects of systemically administered drugs[J]. Cancer Res, 1971,31(6):851-853
[5] Zhang AJ, Li XH, Wang MN . Effect of capsaicin on psoriasis animal model[J]. Zhongguo Pi Fu Xing Bing Xue Za Zhi, 2004,18(6):333-335
张爱军, 李兴华, 王美纳 . 辣椒素对银屑病小鼠模型的作用[J]. 中国皮肤性病学杂志, 2004,18(6):333-335
[6] Liu MQ . Inhibitory effects of different dosage of aqueous extract of Radix Lithospermi in epithelial cell proliferation of mice[J]. Lin Chuang Pi Fu Ke Za Zhi, 2003,32(12):714-715
刘梅青 . 不同剂量紫草水提液对实验性鼠阴道上皮细胞过度增殖抑制作用的初步研究[J]. 临床皮肤科杂志, 2003,32(12):714-715
[7] Wu J, Zhang GW, Huang H, Xu JP . The trial treatment of psoriasis animal model with arotinoid ethylester[J]. Chongqing Yi Xue, 2003,31(3):203-204
吴军, 张国威, 黄海, 许建平 . 芳维A酸乙酯对小鼠银屑病模型的实验治疗.[J]. 重庆医学, 2003,31(3):203-204
[8] Zhou M, Wang JH . Experimental research on psoriasis animal model by traditional Chinese medicine[J]. Liaoning Zhong Yi Za Zhi, 2008,35(2):316-317
周萌, 王建辉 . 中医药对银屑病动物模型的实验研究.[J]. 辽宁中医杂志, 2008,35(2):316-317
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