人参三七组方对人脐静脉内皮细胞血管内皮生长因子分泌及其受体2表达的影响

doi:10.3736/jcim20100412

Abstract

Objective

To investigate the effects of Radix Ginseng and Radix Notoginseng formula on secretion of vascular endothelial growth factor (VEGF) and expression of vascular endothelial growth factor receptor-2 (VEGFR-2) in human umbilical vein endothelial cells (HUVECs) in vitro.
Methods

HUVECs were cultured in vitro. Bovine basic fibroblast growth factor (bFGF) at concentration of 320 U/mL and Radix Ginseng and Radix Notoginseng formula at concentrations of 0.1, 0.2 and 0.4 mg/mL were used to culture with HUVECs. And HUVECs in blank control group were cultured with culture solution only. After 24-hour culture, the content of VEGF in supernatant was detected by enzyme-linked immunosorbent assay and the expression of VEGFR-2 was detected by immunocytochemical staining and Western-blotting.
Results

Radix Ginseng and Radix Notoginseng formula at 0.4 mg/mL, the same as bFGF, increased VEGF content in the HUVEC supernatant and the number of VEGFR-2-positive HUVECs. Expression of VEGFR-2 protein in high-dose Radix Ginseng and Radix Notoginseng formula group was up-regulated as compared with the blank control group.
Conclusion

Radix Ginseng and Radix Notoginseng formula can promote HUVEC proliferation and secretion of VEGF, as well as the expression of VEGFR-2 protein, which may be one of the mechanisms of Radix Ginseng and Radix Notoginseng formula in promoting angiogenesis.

Key words: Drugs, Chinese herbal, Vascular endothelial growth factor, Vascular endothelial growth factor receptor-2, Endothelial cell

Yan Lei , Wei Tian, Lin-qun Zhu, Jing Yang, Ke-ji Chen. Effects of Radix Ginseng and Radix Notoginseng formula on secretion of vascular endothelial growth factor and expression of vascular endothelial growth factor receptor-2 in human umbilical vein endothelial cells. Journal of Chinese Integrative Medicine, 2010, 8(4): 368-372.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20100412

http://www.jcimjournal.com/EN/Y2010/V8/I4/368

TrendMD

Figures/Tables 3

Table 1

Figure 1

Figure 2

References 11

[1]	Lazarous DF, Shou M, Stiber JA, Hodge E, Thirumurti V , Gonçalves L, Unger EF . Adenoviral-mediated gene transfer induces sustained pericardial VEGF expression in dogs: effect on myocardial angiogenesis[J]. Cardiovasc Res, 1999,44(2):294-302 doi: 10.1016/S0008-6363(99)00203-5
[2]	Lei Y, Gao Q, Chen KJ . Effects of extracts from Panax Notoginseng and Panax Ginseng fruit on vascular endothelial cell proliferation and migration in vitro[J]. Chin J Integr Med, 2008,14(1):37-41 doi: 10.1007/s11655-008-0037-0
[3]	Tian W, Lei Y . Research progress of traditional Chinese medicine in therapeutic angiogenesis[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2008,14(4):319-321
	田伟, 雷燕 . 中医药在治疗性血管生成中的研究进展[J]. 中国中医基础医学杂志, 2008,14(4):319-321
[4]	Lei Y, Wang PL, Lin YL, Chen KJ . Danggui Buxue Decoction promotes angiogenesis in ischemic myocardium of aging rats with myocardial infarction[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2005,11(12):892-894
	雷燕, 王培利, 林燕林, 陈可冀 . 当归补血汤煎剂对实验性心肌梗死衰老大鼠缺血心肌的促血管生成作用[J]. 中国中医基础医学杂志, 2005,11(12):892-894
[5]	Liu Y, Liu LX . Recognition of the effects of ginseng[J]. Shi Zhen Guo Yi Guo Yao, 2006,17(2):289
	刘郁, 刘连新 . 人参功效再认识[J]. 时珍国医国药, 2006,17(2):289
[6]	Zhang XP, Qi LL, Liu DR . Research status of the pharmacological effects of Radix Notoginseng and its effective components[J]. Yi Xue Yan Jiu Za Zhi, 2007,36(4):96-98
	张喜平, 齐丽丽, 刘达人 . 三七及其有效成分的药理作用研究现状[J]. 医学研究杂志, 2007,36(4):96-98
[7]	Ferrara N, Gerber HP , LeCouter J . The biology of VEGF and its receptors[J]. Nat Med, 2003,9(6):669-676 doi: 10.1038/nm0603-669
[8]	La DS, Belzile J, Bready JV, Coxon A , DeMelfi T, Doerr N, Estrada J, Flynn JC, Flynn SR, Graceffa RF . Novel 2,3-dihydro-1,4-benzoxazines as potent and orally bioavailable inhibitors of tumor-driven angiogenesis[J]. J Med Chem, 2008,51(6):1695-1705 doi: 10.1021/jm701129j
[9]	Weiss MM, Harmange JC, Polverino AJ, Bauer D, Berry L, Berry V, Borg G, Bready J, Chen D, Choquette D . Evaluation of a series of naphthamides as potent, orally active vascular endothelial growth factor receptor-2 tyrosine kinase inhibitor[J]. J Med Chem, 2008,51(6):1668-1680 doi: 10.1021/jm701098w
[10]	Brekken RA, Overholser JP, Stastny VA, Waltenberger J, Minna JD, Thorpe PE . Selective inhibition of vascular endothelial growth factor(VEGF) receptor 2(KDR/Flk 1) activity by a monoclonal anti-VEGF antibody blocks tumor growth in mice[J]. Cancer Research, 2000,60(18):5117-5124
[11]	Marioni G, Giacomelli L , D’Alessandro E, Staffiere C Guzzardo V, Staffieri A, Blamdamura S . Laryngeal carcinoma recurrence rate and disease-free interval are related to CD105 expression but not to vascular endothelial growth factor 2(Flk-1/Kdr) expression[J]. Anticancer Res, 2008,28(1B):551-557

Related Articles 15

[1]	Shu-yi Chen, Qun-wei Chen, Liu-mei Shou, Hong Pan, Shan-ming Ruan, Zhe-hao Liang, Qi-jin Shu. Stevens-Johnson syndrome/toxic epidermal necrolysis successfully treated with Chinese herbal medicine Pi-Yan-Ning: A case report. Journal of Integrative Medicine, 2021, 19(6): 555-560.
[2]	Xin Yin, Shu-bin Cai, Lan-ting Tao, Lu-ming Chen, Zhong-de Zhang, Su-hong Xiao, Arthur Yin Fan, Xu Zou. Recovery of a patient with severe COVID-19 by acupuncture and Chinese herbal medicine adjuvant to standard care. Journal of Integrative Medicine, 2021, 19(5): 460-466.
[3]	Qing-lan Wang, Yan-yan Tao, Hong-dong Xie, Cheng-hai Liu, Ping Liu. Fuzheng Huayu recipe, a traditional Chinese compound herbal medicine, attenuates renal interstitial fibrosis via targeting the miR-21/PTEN/AKT axis. Journal of Integrative Medicine, 2020, 18(6): 505-513.
[4]	Ming-sheng Lyu, De-ying Li, Shao-zhong Zhou, Cheng-jun Ban, Jun Yan. Adult-onset Still's disease successfully treated with Chinese herbal medicine: A case report with 15-month follow-up. Journal of Integrative Medicine, 2020, 18(6): 530-534.
[5]	Arthur Yin Fan, Sherman Gu, Sarah Faggert Alemi, Research Group for Evidence-based Chinese Medicine. Chinese herbal medicine for COVID-19: Current evidence with systematic review and meta-analysis. Journal of Integrative Medicine, 2020, 18(5): 385-394.
[6]	Bin Lan, Jin-wen Ge, Shao-wu Cheng, Xi-long Zheng, Jun Liao, Chao He, Zheng-qing Rao, Guo-zuo Wang. Extract of Naotaifang, a compound Chinese herbal medicine, protects neuron ferroptosis induced by acute cerebral ischemia in rats. Journal of Integrative Medicine, 2020, 18(4): 344-350.
[7]	Deng-hai Zhang, Kun-lun Wu, Xue Zhang, Sheng-qiong Deng, Bin Peng. In silico screening of Chinese herbal medicines with the potential to directly inhibit 2019 novel coronavirus. Journal of Integrative Medicine, 2020, 18(2): 152-158.
[8]	Fang-yuan Wang, Jian Jia, Huan-huan Song, Cheng-ming Jia, Chang-bo Chen, Jing Ma. Icariin protects vascular endothelial cells from oxidative stress through inhibiting endoplasmic reticulum stress. Journal of Integrative Medicine, 2019, 17(3): 205-212.
[9]	Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
[10]	Carola Analía Torres, Cristina Marisel Pérez Zamora, María Beatriz Nuñez, Ana María Gonzalez. In vitro antioxidant, antilipoxygenase and antimicrobial activities of extracts from seven climbing plants belonging to the Bignoniaceae. Journal of Integrative Medicine, 2018, 16(4): 255-262.
[11]	Shui-jie Shen, Yong-hong Zhang, Xiao-xia Gu, Shui-ju Jiang, Ling-jun Xu. Yangfei Kongliu Formula, a compound Chinese herbal medicine, combined with cisplatin, inhibits growth of lung cancer cells through transforming growth factor-β1 signaling pathway. Journal of Integrative Medicine, 2017, 15(3): 242-251.
[12]	Liliana Zazueta-Beltrán, Lorena Medina-Aymerich, Nadia Estela Díaz-Triste, Aracely Evangelina Chávez-Piña, Gilberto Castañeda-Hernández, Leticia Cruz-Antonio. Evidence against the participation of a pharmacokinetic interaction in the protective effect of single-dose curcumin against gastrointestinal damage induced by indomethacin in rats. Journal of Integrative Medicine, 2017, 15(2): 151-157.
[13]	Tom Fleischer, Tung-Ti Chang, Hung-Rong Yen. Post-hematopoietic stem cell transplantation in patients with hematologic disorders: Chinese herbal medicine for an unmet need. Journal of Integrative Medicine, 2016, 14(5): 322-335.
[14]	JiaXia, Li-qunHe, XiaoSu. Interventional mechanisms of herbs or herbal extracts on renal interstitial fibrosis. Journal of Integrative Medicine, 2016, 14(3): 165-173.
[15]	Mojtaba Heydari, Mohammad Hashem Hashempur, Mohammad Hosein Ayati, Detlev Quintern, Majid Nimrouzi, Seyed Hamdollah Mosavat. The use of Chinese herbal drugs in Islamic medicine. Journal of Integrative Medicine, 2015, 13(6): 363-367.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2]	Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3]	Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4]	Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5]	Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6]	Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7]	Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8]	Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9]	Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10]	Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278

Group	VEGF (ng/L,n=8)	Number of VEGFR-2-positive HUVECs (n=6)	OD value of VEGFR-2-positive HUVECs (n=6)	VEGFR-2/β-actin (n=3)
Blank control	67.61±8.54	13.18±5.79	0.149±0.002	1.583 9±0.102 8
bFGF	93.68±11.35^*	18.08±6.06^**	0.183±0.002^*	2.113 9±0.211 5^*
High-dose formula	89.24±12.16^*	18.29±4.98^**	0.171±0.003^*	2.074 5±0.189 8^*
Medium-dose formula	74.57±8.90^△	13.81±4.31^△△□□	0.168±0.003^*	1.482 2±0.173 0
Low-dose formula	72.81±9.26^△	12.79±4.10^△△□□	0.147±0.003^△▲□	1.388 8±0.262 0

Effects of Radix Ginseng and Radix Notoginseng formula on secretion of vascular endothelial growth factor and expression of vascular endothelial growth factor receptor-2 in human umbilical vein endothelial cells

RichHTML

PDF (PC)

Like