益气增敏方对2型糖尿病大鼠骨骼肌组织葡萄糖转运蛋白4表达的影响

doi:10.3736/jcim20111015

Abstract

Objective: To investigate the effects of traditional Chinese compound recipe Yiqi Zengmin (YQZM) formula on expression of glucose transporter 4 (GLUT4) in skeletal muscle of rats with type 2 diabetes induced by high-fat diet combined with low-dose-streptozotocin injection.

Methods: Fifty male rats were randomly divided into two groups: model group fed with high-fat diet (n=40) and control group (n=10). After 4-week feed of high-fat diet, the rats of the model group were injected with streptozotocin at a dose of 35 mg/kg. Rats with plasma glucose over 16.67 mmol/L were randomly divided into 4 groups: diabetic model group, YQZM group, rosiglitazone group and losartan group and were treated with water, YQZM, rosiglitazone or losartan respectively by oral administration for 8 weeks. The expression of GLUT4 protein in the cytoplasm and the plasma membrane of isolated rats’ skeletal muscles were detected by Western blotting.

Results: The expression of GLUT4 in the plasma membrane was more than that in the cytoplasm in skeletal muscle tissues of the normal rats, while the expression of GLUT-4 in the plasma membrane was less than that in the cytoplasm in skeletal muscle tissues of the type 2 diabetic rats. YQZM formula enhanced GLUT4 translocation from the cytoplasm to the plasma membrane in skeletal muscle tissues, similar to rosiglitazone.

Conclusion: YQZM formula can enhance GLUT4 translocation from the cytoplasm to the plasma membrane in skeletal muscle tissues, and displays the insulin sensitization characteristic of rosiglitazone.

Key words: compounds (TCD), diabetes mellitus,type 2, glucose transporter 4, rosiglitazone, losartan, rats

Hong-li Xue, Zeng Zhang, Wen-jian Wang, Wei-hua Chen, Jian Ying. Effects of Chinese herbal medicine Yiqi Zengmin Formula on expression of glucose transporter 4 in skeletal muscle in type 2 diabetic rats. Journal of Chinese Integrative Medicine, 2011, 9(10): 1133-1137.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20111015

http://www.jcimjournal.com/EN/Y2011/V9/I10/1133

[1]	Wang WJ . Foundation and application of aggregation syndrome theory in traditional Chinese medicine[J]. J Chin Integr Med, 2007,5(2):111-114
	王文健 . 聚证学说的建立和应用[J]. 中西医结合学报, 2007,5(2):111-114
[2]	Lou SY, Liu Y, Ma YY, Chen HY, Chen WH, Ying J, He YM, Wang WJ . Effects of Yiqi Sanju formula on non-alcoholic fatty liver disease: a randomized controlled trial[J]. J Chin Integr Med, 2008,6(8):793-798
	娄少颖, 刘毅, 马宇颖, 陈海英, 陈伟华, 应健, 何燕铭, 王文健 . 益气散聚方治疗非酒精性脂肪性肝病随机对照临床试验[J]. 中西医结合学报, 2008,6(8):793-798
[3]	He CY, Fu XD, Li F, He YM, Chen WH, Ying J, Wang WJ . Effects of “Yiqi Sanju Formula” on levels of some adipocytokines in central obese men[J]. Shanghai Zhong Yi Yao Za Zhi, 2007,41(8):16-18
	何春燕, 傅晓东, 李玢, 何燕铭, 陈伟华, 应健, 王文健 . 益气散聚方对中心性肥胖男性相关脂肪细胞因子水平的影响[J]. 上海中医药杂志, 2007,41(8):16-18
[4]	Lou SY, Liu Y, Wang WJ . Successful treatment of three cases of early diabetes mellitus by tonifying qi and dispersing accumulation[J]. Shanghai Zhong Yi Yao Za Zhi, 2007,41(7):27-28
	娄少颖, 刘毅, 王文健 . 益气散聚法治疗早期糖尿病验案3则[J]. 上海中医药杂志, 2007,41(7):27-28
[5]	Zhang Z, Xue HL, Liu Y, Wang WJ . Yi-Qi-Zeng-Min-Tang, a Chinese medicine, ameliorates insulin resistance in type 2 diabetic rats[J]. World J Gastroenterol, 2011,17(8):987-995 doi: 10.3748/wjg.v17.i8.987 pmid: 21448349
[6]	Astrup A, Buemann B, Western P, Toubro S, Raben A, Christensen NJ . Obesity as an adaptation to a high-fat diet: evidence from a cross-sectional study[J]. Am J Clin Nutr, 1994,59(2):350-355 doi: 10.1079/PNS19940072 pmid: 7993398
[7]	Zhang F, Ye C, Li G, Ding W, Zhou W, Zhu H, Chen G, Luo T, Guang M, Liu Y, Zhang D, Zheng S, Yang J, Gu Y, Xie X, Luo M . The rat model of type 2 diabetic mellitus and its glycometabolism characters[J]. Exp Anim, 2003,52(5):401-407 doi: 10.1538/expanim.52.401 pmid: 14625406
[8]	Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P . Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: A model for type 2 diabetes and pharmacological screening[J]. Pharmacol Res, 2005,52(4):313-320 doi: 10.1016/j.phrs.2005.05.004
[9]	Pearson SL, Cawthorne MA, Clapham JC, Dunmore SJ, Holmes SD, Moore GB, Smith SA, Tadayyon M . The thiazolidinedione insulin sensitizer, BRL 49653, increases the expression of PPARγ and aP2 in adipose tissue of high-fat-fed rats[J]. Biochem Biophys Res Commun, 1996,229(3):752-757 doi: 10.1006/bbrc.1996.1876
[10]	Barmann M, Hermans JJ, Janssen BJ, Peutz-Kootstra C, Witzke O, Heemann U, Smits JF, Boudier HA . Transient prehypertensive treatment in spontaneously hypertensive rats: a comparison of spironolactone and losartan regarding long-term blood pressure and target organ damage[J]. J Hypertens, 2007,25(12):2504-2511 doi: 10.1097/HJH.0b013e3282ef84f8
[11]	Huisamen B, van Zyl M, Keyser A, Lochner A . The effects of insulin and beta-adrenergic stimulation on glucose transport, glut 4 and PKB activation in the myocardium of lean and obese non-insulin dependent diabetes mellitus rats[J]. Mol Cell Biochem, 2001,223(1-2):15-25 doi: 10.1023/A:1017528402205
[12]	Sun CH, Zhou XR, Wen Y, Liu YM . Effects of conjugated linoleic acid on expression of GLUT4 protein in skeletal muscle of insulin resistant rat[J]. Zhonghua Yu Fang Yi Xue Za Zhi, 2007,41(1):25-28 doi: 10.3760/j:issn:0253-9624.2007.01.007
	孙长颢, 周晓蓉, 闻颖, 刘岩梅 . 共轭亚油酸对胰岛素抵抗大鼠葡萄糖运载体4蛋白表达影响的研究[J]. 中华预防医学杂志, 2007,41(1):25-28 doi: 10.3760/j:issn:0253-9624.2007.01.007
[13]	Guo XH, Liu ZH, Li H, Li LS . Type 2 diabetes mellitus induced by diets and its features of renal involvement in rat[J]. Zhongguo Tang Niao Bing Za Zhi, 2002,10(5):290-294
	郭啸华, 刘志红, 李恒, 黎磊石 . 高糖高脂饮食诱导的2型糖尿病大鼠模型及其肾病特点[J]. 中国糖尿病杂志, 2002,10(5):290-294
[14]	Li YS, Wang WJ, Ma YY, Liu Y, He CY, He YM, Chen WH, Ying J . Effect of Compound Danshen Drop Pills on early stage diabetic nephropathy in rat models[J]. Zhong Cheng Yao, 2007,29(1):40-44 doi: 10.3969/j.issn.1001-1528.2007.01.012
	李佑生, 王文健, 马宇滢, 刘毅, 何春燕, 何燕铭, 陈伟华, 应健 . 复方丹参滴丸防治大鼠早期糖尿病肾脏损害的研究[J]. 中成药, 2007,29(1):40-44 doi: 10.3969/j.issn.1001-1528.2007.01.012
[15]	Wang WJ . On lump syndrome theory and its clinical application[J]. Shanghai Zhong Yi Yao Za Zhi, 2008,42(7):60-62
	王文健 . 论聚证学说及临证辨治[J]. 上海中医药杂志, 2008,42(7):60-62
[16]	Maianu L, Keller SR, Garvey WT . Adipocytes exhibit abnormal subcellular distribution and translocation of vesicles containing glucose transporter 4 and insulin-regulated aminopeptidase in type 2 diabetes mellitus: implications regarding defects in vesicle trafficking[J]. J Clin Endocrinol Metab, 2001,86(11):5450-5456 doi: 10.1210/jcem.86.11.8053
[17]	Wang SH, Wang WJ, Wang XF, Chen WH . Effect of Astragalus polysaccharides and berberine on carbohydrate metabolism and cell differentiation in 3T3-L1 adipocytes[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2004,24(10):926-928 doi: 10.3321/j.issn:1003-5370.2004.10.017
	王树海, 王文健, 汪雪峰, 陈伟华 . 黄芪多糖和小檗碱对3T3-L1脂肪细胞糖代谢及细胞分化的影响[J]. 中国中西医结合杂志, 2004,24(10):926-928 doi: 10.3321/j.issn:1003-5370.2004.10.017

Related Articles 15

[1]	Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[2]	Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[3]	Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[4]	Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[5]	Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[6]	Bing He, Ge Zhang, Ai-ping Lu. Integrative network analysis: Bridging the gap between Western medicine and traditional Chinese medicine. Journal of Integrative Medicine, 2015, 13(3): 133-135.
[7]	Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[8]	Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[9]	Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[10]	Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[11]	Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[12]	Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[13]	Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[14]	Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[15]	Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng . Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2]	Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3]	Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4]	Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5]	A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H₂₂ cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6]	Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7]	Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8]	Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9]	Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10]	Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022

Effects of Chinese herbal medicine Yiqi Zengmin Formula on expression of glucose transporter 4 in skeletal muscle in type 2 diabetic rats

RichHTML

PDF (PC)

Like

Abstract

Cite this article

Share this article

TrendMD

Figures/Tables 1

References 17

Related Articles 15

Metrics

Comments

Recommended 10