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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (10): 1133-1137.doi: 10.3736/jcim20111015

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Yiqi Zengmin Formula on expression of glucose transporter 4 in skeletal muscle in type 2 diabetic rats

Hong-li Xue(), Zeng Zhang, Wen-jian Wang, Wei-hua Chen, Jian Ying   

  1. Institute of Chinese Integrative Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2011-05-08 Accepted:2011-06-09 Online:2011-10-20 Published:2011-10-15
  • Contact: Xue Hong-li

Objective: To investigate the effects of traditional Chinese compound recipe Yiqi Zengmin (YQZM) formula on expression of glucose transporter 4 (GLUT4) in skeletal muscle of rats with type 2 diabetes induced by high-fat diet combined with low-dose-streptozotocin injection.

Methods: Fifty male rats were randomly divided into two groups: model group fed with high-fat diet (n=40) and control group (n=10). After 4-week feed of high-fat diet, the rats of the model group were injected with streptozotocin at a dose of 35 mg/kg. Rats with plasma glucose over 16.67 mmol/L were randomly divided into 4 groups: diabetic model group, YQZM group, rosiglitazone group and losartan group and were treated with water, YQZM, rosiglitazone or losartan respectively by oral administration for 8 weeks. The expression of GLUT4 protein in the cytoplasm and the plasma membrane of isolated rats’ skeletal muscles were detected by Western blotting.

Results: The expression of GLUT4 in the plasma membrane was more than that in the cytoplasm in skeletal muscle tissues of the normal rats, while the expression of GLUT-4 in the plasma membrane was less than that in the cytoplasm in skeletal muscle tissues of the type 2 diabetic rats. YQZM formula enhanced GLUT4 translocation from the cytoplasm to the plasma membrane in skeletal muscle tissues, similar to rosiglitazone.

Conclusion: YQZM formula can enhance GLUT4 translocation from the cytoplasm to the plasma membrane in skeletal muscle tissues, and displays the insulin sensitization characteristic of rosiglitazone.

Key words: compounds (TCD), diabetes mellitus,type 2, glucose transporter 4, rosiglitazone, losartan, rats

Figure 1

Expression of GLUT4 protein in cytoplasm and membrane of skeletal muscle cells in each group1: Control group; 2: Type 2 diabetes model group; 3: Yiqi Zengmin group; 4: Losartan group; 5: Rosiglitazone group. GLUT4: glucose transporter 4; GAPDH: glyceraldehyde-3-phosphate dehydrogenase. *, P<0.05, **, P<0.01."

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