Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (12): 1164-1166.doi: 10.3736/jcim20091214

• Original Experimental Research • Previous Articles     Next Articles

Effects of Qingshi Cream on chronic dermatitis-eczema in mice

 Yong-mei Li, Xiao-rui Li   

  1. Department of Dermatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2009-09-02 Accepted:2009-10-30 Online:2009-12-20 Published:2009-12-15
  • Contact: Yong-mei Li


To explore the effects of Qingshi Cream, a compound traditional Chinese herbal medicine, on chronic dermatitis-eczema in mice induced by 2,4-dinitrochlorobenzene (DNCB).

Thirty BALB/C mice were randomly divided into vaseline group, 0.1% mometasone furoate cream group and Qingshi Cream group. Right ears of BALB/C mice were repeatedly challenged with 0.1% DNCB every three days for five times and previously sensitized with 7% DNCB to induce chronic dermatitis-eczema. Mice in different groups were applied Qingshi Cream, 0.1% mometasone furoate cream and vaseline respectively after each challenge. Weight difference of two ears, pathological change of right ear and dermal inflammatory cell number were used to assess the effects of the drugs.

After the 5th challenge, weight differences of two ears in the 0.1% mometasone furoate cream group and the Qingshi Cream group were significantly decreased as compared with that in the vaseline group. Changes such as ear swelling thickening and cellular infiltration in dermis were observed, and these features seemed to be more significant in the vaseline group as compared with the 0.1% mometasone furoate cream group and the Qingshi Cream group.

Qingshi Cream has an obvious effect in treatment of chronic dermatitis-eczema in mice.

Key words: Chronic dermatitis-eczema, Qingshi Cream, Mice

Table 1

Weight difference of two ears of mice in different groups ($\bar{x}$±s,mg)"

Group n Weight difference of two ears
Vaseline 10 8.98±0.204
0.1% mometasone furoate cream 10 1.59±0.137**
Qingshi Cream 10 1.52±0.155**

Table 2

Dermal inflammatory cell number of right ear of mice in different groups ($\bar{x}$±s,cells/mm2)"

Group n Dermal inflammatory cell number of right ear
Vaseline 6 84.0±4.10
0.1% mometasone furoate cream 6 32.0±3.57**
Qingshi Cream 6 28.5±4.23**

Figure 1

Pathological change of right ear in mice observed by light microscopy(HE staining, ×200) A: Vaseline group; B: 0.1% mometasone furoate cream group; C: Qingshi Cream group; D: Normal tissue."

[1] Jin HL, Zhang RZ, Yu QB . Model of chronic dermatitis-eczema in mice[J]. Bengbu Yi Xue Yuan Xue Bao, 2002,27(3):193-194
金慧玲, 张汝芝, 余其斌 . 小鼠慢性皮炎-湿疹模型的建立[J]. 蚌埠医学院学报, 2002,27(3):193-194
[2] Lin YG, Wu YH, Yang RJ, Su ZH, Yang XH . Clinical observation on psoriatic treated by complex prescription Qingdai basilicon[J]. Chengdu Zhong Yi Yao Da Xue Xue Bao, 2006,29(2):13-16
林胤谷, 吴宜鸿, 杨荣季, 苏中慧, 杨贤鸿 . 复方青黛油膏治疗银屑病的临床疗效评估[J]. 成都中医药大学学报, 2006,29(2):13-16
[3] Liu YF, Li X .The application history and modern research summary of calcined gypsum[J].Zhong Yi Yao Xue Kan, 2005, 23(1): 47, 50
刘元芬, 李祥 . 煅石膏应用沿革及现代研究述要[J].中医药学刊, 2005, 23(1): 47, 50
[4] Zhu YP, Zhao SC . Analysis on mineral elements in Calamitas urinas hominis[J]. Guangdong Wei Liang Yuan Su Ke Xue, 2003,10(10):53-55
朱远平, 赵书策 . 人中白矿物元素含量分析[J]. 广东微量元素科学, 2003,10(10):53-55
[5] Zhai WH, Ma FC, Chao HM . Progress on traditional Chinese medicine borax[J]. Dong Wu Yi Xue Jin Zhan, 2007,28(8):87-91
翟卫红, 马富春, 晁宏梅 . 中药硼砂研究进展[J]. 动物医学进展, 2007,28(8):87-91
[6] Li B, Zhu WL, Chen KX . Advances in the study of berberine and its derivatives[J]. Yao Xue Xue Bao, 2008,43(8):773-787
李波, 朱维良, 陈凯先 . 小檗碱及其衍生物的研究进展[J]. 药学学报, 2008,43(8):773-787
[7] Liu HJ, Jin RM . Advances in the study of peppermint oil[J]. Shandong Zhong Yi Yao Da Xue Xue Bao, 2006,30(6):502-505
刘红杰, 金若敏 . 薄荷油研究进展[J]. 山东中医药大学学报, 2006,30(6):502-505
[1] Yi Ran Hu, San Li Xing, Chuan Chen, Ding Zhu Shen, Jiu Lin Chen. Tiaoxin Recipe, a Chinese herbal formula, inhibits microRNA-34a expression in the APPswe/PS1ΔE9 mouse model of Alzheimer's disease. Journal of Integrative Medicine, 2019, 17(6): 404-409.
[2] Bhesh Raj Sharma, Dong Wook Kim, Dong Young Rhyu. Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2017, 15(1): 56-63.
[3] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[4] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[5] Md. Asadujjaman, Ahmed Ullah Mishuk, Md. Aslam Hossain, Utpal Kumar Karmakar. Medicinal potential of Passiflora foetida L. plant extracts: Biological and pharmacological activities. Journal of Integrative Medicine, 2014, 12(2): 121-126.
[6] Marjan Nassiri-Asl, Taghi Naserpour Farivar, Esmail Abbasi, Hamid Reza Sadeghnia, Mehdi Sheikhi, Mina Lotfizadeh, Parisa Bazahang. Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. Journal of Chinese Integrative Medicine, 2013, 11(5): 337-342.
[7] Md. Khirul Islam, Imran Mahmud, Sanjib Saha, Asit Baron Sarker, Himangsu Mondal, A.S.M. Monjur-Al-Hossain, Md. Anisuzzman. Preliminary pharmacological evaluation of Alocasia indica Schott tuber. Journal of Chinese Integrative Medicine, 2013, 11(5): 343-351.
[8] Sanjib Saha, Faroque Hossain, Md. Anisuzzman, Md. Khirul Islam. Pharmacological evaluation of Musa seminifera Lour. Fruit. Journal of Chinese Integrative Medicine, 2013, 11(4): 253-261.
[9] Sunil A. Nirmal, Jayashri M. Ingale, Shashikant R. Pattan, Sanjay B. Bhawar. Amaranthus roxburghianus root extract in combination with piperine as a potential treatment of ulcerative colitis in mice. Journal of Chinese Integrative Medicine, 2013, 11(3): 206-212.
[10] Lin Wang, Guan-liang Wang, Jia-han Liu, Di Li, De-zeng Zhu, Liang-neng Wu . Effects of ursolic acid in ameliorating insulin resistance in liver of KKAy mice via peroxisome proliferator-activated receptors α and γ. Journal of Chinese Integrative Medicine, 2012, 10(7): 793-799.
[11] Yi-tao Zhao , Xin-guang Zhang , Li Bai , Li-qing Li , Jian-er Yu . Effects of Chinese herbal medicine Pingchuan Formula on airway inflammation, interferon-γ and interleukin-4 in mice with asthma. Journal of Chinese Integrative Medicine, 2012, 10(7): 807-813.
[12] Mei-jiao Mao, Jun-ping Hu, Cong Wang, Yi-yi Zhang, Ping Liu. Effects of Chinese herbal medicine Guanxinkang on expression of PPARγ-LXRα-ABCA1 pathway in ApoE-knockout mice with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(7): 814-820.
[13] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
[14] Surendra Kr. Sharma, Naveen Goyal. Protective effect of Heliotropium eichwaldi against cisplatin-induced nephrotoxicity in mice. Journal of Chinese Integrative Medicine, 2012, 10(5): 555-560.
[15] Jing Guo, Ju-yong Wang, Zhan Zheng, Qing Wang, Chang-sheng Dong . Effects of Chinese herbal medicine Feiyanning Decoction on the ratio of CD4+CD25+ regulatory T cells and expression of transcription factor Foxp3 in mice bearing Lewis lung carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(5): 584-590.
Full text



[1] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[2] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[3] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[4] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[5] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[6] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[7] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[8] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[9] Bing Hu , Hong-mei An , Ke-ping Shen , Qin Du. Senescence-inducing effects of Chinese herbal medicine Tenglong Buzhong Decoction on human colon carcinoma LS-174-T cells and the mechanism. Journal of Chinese Integrative Medicine, 2010, 8(11): 1048-1052
[10] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079