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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (6): 466-469.doi: 10.3736/jcim20050612

• Original Clinical Research • Previous Articles     Next Articles

Effects of Yiqi Huayu Recipe and its decomposed formulas on apoptosis-related factors of anulus fibrosus cells in rats

Quan Zhou1,Yong-jun Wang1,Qi Shi1,Peng Sun2,Chong-jian Zhou1,Zhi-jun Hu1,Mei Liu1   

  1. 1. Spinal Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. College of Acupuncture and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Online:2005-11-20 Published:2005-11-20
  • Contact: Yong-Jun WANG


To study the effects of Yiqi Huayu Recipe and its decomposed formulas-medicated sera on expressions of bcl-2, Bax and caspase-8 of apoptotic anulus fibrosus cells in rats.


Immunohistochemical and integral optical density analytic methods were used to observe the effects of Yiqi Huayu Recipe-, Yiqi Recipe-, Huayu Recipe-medicated sera and insulin-like growth factor 1 (IGF-1) on the expressions of bcl-2, Bax and caspase-8 of apoptotic anulus fibrosus cells in rats induced by anti-Fas antibody.


As compared with apoptosis group, bcl-2 expression was higher, Bax and caspase-8 expressions were lower in Yiqi Huayu-treated, Yiqi-treated, Huayu-treated and IGF-1 groups (P<0.01). As compared with Yiqi-treated group and Huayu-treated group, Bax expression was lower in Yiqi Huayu-treated group (P<0.05).


Yiqi Huayu Recipe and its decompsed formulas can delay degeneration of the cervical intervertebral disc, which may be due to its action in regulating the expressions of bcl-2, Bax and caspase-8.

Key words: Yiqi Huayu Recipe, Intervertebral disc, Anulus fibrosus, Bcl-2, Bax, Caspase-8

CLC Number: 

  • R289.11

Tab 1

Integral optical density results of bcl-2, Bax and caspase-8 ($\bar{x}$±s )"

Group n bcl-2 caspase-8 Bax
Normal control 6 1199.05±233.78▲▲ 91.46±10.35▲▲ 81.48±21.42▲▲
Apoptosis 6 116.02±23.31 1084.25±165.06 1012.39±175.66
YiQi-treated 6 401.78±162.19△△▲▲ 560.62±84.05△△▲▲ 496.42±92.35*△△▲▲
Huayu-treated 6 339.89±103.94△△▲▲ 685.33±116.74△△▲▲ 586.56±111.00*△△▲▲
Yiqi Huayu-treated 6 686.23±255.39△▲▲ 260.19±34.20△▲▲ 237.28±57.71△▲▲
IGF-1 6 741.63±250.09△▲▲ 187.57±51.06▲▲ 148.91±28.07▲▲
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