Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (3): 302-306.doi: 10.3736/jcim20070314

• Original Experimental Research • Previous Articles     Next Articles

Effects of Jianpi Huoxue Decoction on Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and lipopolysaccharide

Jing-hua Peng, Zhi-hong Fang, Jian-wei Cui, Qin Feng, Li-li Xu, Hong-tu Gu, Yi-yang Hu   

  1. Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Online:2007-05-31 Published:2007-05-15

Objective: To observe the effects of Jianpi Huoxue Decoction, a compound Chinese herbal medicine, on Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and lipopolysaccharide (LPS).Methods: SD rats were divided into normal, control liquid diet, ethanol liquid diet and ethanol liquid diet plus Jianpi Huoxue Decoction group. Rats were administrated with Jianpi Huoxue Decoction or distilled water via gastrogavage for 4 weeks after administration with ethanol or control liquid diet for 2 weeks respectively. After that, rats in each group were stimulated with LPS via gastrogavage for 3.5 h and harvested. Alanine aminotransferase (ALT) in serum and triglyceride (TG) in liver were analyzed. Pathological changes in liver tissues were observed in HE staining section. Tumor necrosis factor-α(TNF-α) in portal vein plasma was assayed by ELISA. The protein expressions of CD68, Toll-like receptor 4 (TLR4), phosphorylation-IκB (P-IκB) and TNF-α in liver were evaluated with Western-blotting.Result: After the treatment with Jianpi Huoxue Decoction, the pathologic changes in liver tissue were lightened, levels of ALT in serum, TG in liver and TNF-α in portal vein plasma were decreased, and the protein expressions of CD68, TLR4, P-IκB and TNF-α in liver were reduced.Conclusion: Jianpi Huoxue Decoction can inhibit Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and LPS.

Key words: liver disease, alcoholic, endotoxin, lipopolysaccharides, tumor necrosis factor-α

CLC Number: 

  • R575

Figure 1

HE staining for liver tissue and immunohistochemical staining for CD68 (×200)A: HE staining for liver tissue; A1: Normal group; A2: Control liquid diet group; A3: Ethanol liquid diet group; A4: Jianpi Huoxue Decoction group. B: Immunohistochemical staining for CD68; B1: Normal group; B2: Control liquid diet group; B3: Ethanol liquid diet group; B4: Jianpi Huoxue Decoction group."

Table 1

Contents of TG in liver, TNF-α in portal vein plasma and activity of ALT in serum (ヌ±S)"

Group n TG in liver (mg/g) TNF-α in portal vein plasma (ng/L) ALT in serum (U/L)
Normal 6 38.93±16.51* 14.97±1.52** 39±4**
Control liquid diet 11 75.37±23.30* 15.91±1.19** 24±7**
Ethanol liquid diet 12 365.59±50.42 29.55±3.37 96±22
Jianpi Huoxue Decoction 12 245.59±63.85*△△ 26.00±2.77*△△ 75±18**

Figure 2

Effects of Jianpi Huoxue Decoction on protein expressions of CD68, TLR4, P-IκB and TNF-α in rats with liver injury induced by Lieber-Decarli diet and LPS (Western-blotting)1: Normal group; 2: Control liquid diet group; 3: Ethanol liquid diet group; 4: Jianpi Huoxue Decoction group; *P<0.05, *P<0.01, vs ethanol liquid diet group."

[1] 庄辉 . 酒精性肝病的流行病学[J]. 中华肝脏病杂志, 2003,11(11):689
Zhuang H . Epidemiology of alcoholic liver disease[J]. Zhonghua Gan Zang Bing Za Zhi, 2003,11(11):689
[2] 胡义扬, 方志红, 崔剑巍 , 等. 酒精性肝损伤与小肠通透性改变的关系及中药的干预作用[J]. 科学技术与工程, 2005,5(22):1770-1771, 1776
Hu YY, Fang ZH, Cui JW , et al. Relationship between alcoholic liver injury and gut permeability and the alleviating effect of Chinese herbs[J]. Ke Xue Ji Shu Yu Gong Cheng, 2005,5(22):1770-1771, 1776
[3] Cui JW, Hu YY, Fang ZH , et al. Intervention effects of Jianpi Liqi Huoxue Decoction on lipid peroxidative liver injury induced by alcohol[J]. Chin J Integr Med, 2006,12(4):281-286
doi: 10.1007/s11655-006-0281-0
[4] Tsukamoto H, Lu SC . Current concepts in the pathogenesis of alcoholic liver injury[J]. FASEB J, 2001,15(8):1335-1349
[5] Thurman RG . Mechanisms of Hepatic Toxicity II[J]. Alcoholic liver injury involves activation of Kupffer cells by endotoxin. Am J Physiol Gastrointest Liver Physiol, 1998,275(4):G605-G611
[6] Adachi Y, Moore LE, Bradford BU , et al. Antibiotics prevent liver injury in rats following long-term exposure to ethanol[J]. Gastroenterology, 1995,108(1):218-224
[7] Hansen J, Cherwitz DL, Allen JI . The role of tumor necrosis factor-α in acute endotoxin-induced hepatotoxicity in ethanol-fed rats[J]. Hepatology, 1994,20(2):461-474
[8] Su GL . Lipopolysaccharides in liver injury: molecular mechanisms of Kupffer cell activation[J]. Am J Physiol Gastrointest Liver Physiol, 2002,283(2):256-265
doi: 10.1152/ajpgi.00550.2001 pmid: 12121871
[9] Uesugi T, Froh M, Arteel GE , et al. Toll-like receptor 4 is involved in the mechanism of early alcohol-induced liver injury in mice[J]. Hepatology, 2001,34(1):101-108
doi: 10.1053/jhep.2001.25350 pmid: 11431739
[1] Byung Hyuk Han, Yun Jung Lee, Jung Joo Yoon, Eun Sik Choi, Seung Namgung, Xian Jun Jin, Da Hye Jeong, Dae Gill Kang, Ho Sub Lee. Hwangryunhaedoktang exerts anti-inflammation on LPS-induced NO production by suppressing MAPK and NF-κB activation in RAW264.7 macrophages. Journal of Integrative Medicine, 2017, 15(4): 326-336.
[2] Chang-qing Zhao, Yang Zhou, Jian Ping, Lie-ming Xu. Traditional Chinese medicine for treatment of liver diseases: Progress, challenges and opportunities. Journal of Integrative Medicine, 2014, 12(5): 401-408.
[3] Mao Zhi-min, Song Hai-yan, Yang Li-li, Liu Tao, Li Dong-fei, Zheng Pei-yong, Liu Ping, Ji Guang. Effects of the mixture of Swertia pseudochinensis Hara and Silybum marianum Gaertn extracts on CCl4-induced liver injury in rats with non-alcoholic fatty liver disease. Journal of Chinese Integrative Medicine, 2012, 10(2): 193-199.
[4] Lin-hua Qin, Jiao Lü, Lin Kong, Yun-xing Shi, Yong-ping Li, Guo-zhong Zhou, Zhi-wu Zheng, Lin Li, Xing-ying Ji. Andrographolide inhibits extracellular signal-regulated kinase 1/2 signaling pathway in activated macrophages. Journal of Chinese Integrative Medicine, 2011, 9(6): 632-637.
[5] Tao Liu, Zhi-peng Tang, Guang Ji. Treatment of non-alcoholic fatty liver disease based on traditional Chinese medicine therapies for warming yang to activate qi. Journal of Chinese Integrative Medicine, 2011, 9(2): 135-137.
[6] Qi-lin Fu, Yi-yang Hu, Qin Feng, Xiao-ning Wang, Jing-hua Peng, Tuan Cui. Analysis of major herbs in Chinese herbal formula Jianpi Huoxue Decoction for improving intestinal permeability based on uniform design. Journal of Chinese Integrative Medicine, 2011, 9(11): 1234-1241.
[7] Hua-qiang Ouyang, Lu-ming Liu, Zhen Chen, Jian-min Luo, Er-xin Yu. Effects of Qingyi Huaji decoction on serum levels of interleukin-6, interleukin-8 and tumor necrosis factor-α in nude mice bearing pancreatic tumors. Journal of Chinese Integrative Medicine, 2010, 8(7): 655-661.
[8] Ping Xie, Xin-xi Zhou, Qin Zhang. Pathogenesis and treatment of non-alcoholic fatty liver disease. Journal of Chinese Integrative Medicine, 2010, 8(3): 201-209.
[9] Guang Ji . Pay attention to insulin resistance in chronic liver diseases. Journal of Chinese Integrative Medicine, 2010, 8(1): 1-6.
[10] Jun-ming Chen, Lei Wang, Tao Liu, Lian-jun Xing, Pei-yong Zheng, Guang Ji. Effects of Qinggan Huoxue Recipe and its separated recipes on urokinase-type plasminogen activator and plasminogen activator inhibitor-1 fibrinolytic system in rats with alcoholic liver fibrosis. Journal of Chinese Integrative Medicine, 2009, 7(7): 642-650.
[11] Marjan Nassirl-Asl, Farzaneh Zamansoltiani, Esmail Abbasl, Mohammad-Mehdi Daneshi, Amir-Abdollah Zangivand. Effects of Urtica dioica extract on lipid profile in hypercholesterolemic rats. Journal of Chinese Integrative Medicine, 2009, 7(5): 428-433.
[12] Hui Li , Shai-hui Du, Sai-xia Zhang, Ru-dong Deng, Chun Li, Yi-wei Li, Dong-feng Chen, Jian-hong Zhou . Effects of Niupo Zhibao Pellet on high-mobility group box-1 protein expression in lung tissues of endotoxin shock rats. Journal of Chinese Integrative Medicine, 2009, 7(5): 441-446.
[13] Arthur de Sa Ferreira, Januario Gomes Mouraoe Lima, Tatiana Pereira Teixeira Ferreira, Camila Madeira Tavares Lopes, Rubens Meyer. Prophylactic effects of short-term acupuncture on Zusanli (ST36) in Wistar rats with lipopolysaccharide-induced acute lung injury. Journal of Chinese Integrative Medicine, 2009, 7(10): 969-975.
[14] Hui Zhang, Qin Feng, Hong-shan Li, Shao-dong Chen, Xiao-ning Wang, Jing-hua Peng, Ning Zhang, Yi-yang Hu. Effects of Qushi Huayu Decoction on cathepsin B and tumor necrosis factor-α expression in rats with non-alcoholic steatohepatitis. Journal of Chinese Integrative Medicine, 2008, 6(9): 928-933.
[15] Tao Wu, Tao Liu, Pei-yong Zhang, Lian-jun Xing, Guang Ji. Effects of Qinggan Huoxue Recipe and its separated recipes on the expression of tumor necrosis factor-α in rats with alcoholic liver injury. Journal of Chinese Integrative Medicine, 2008, 6(11): 1145-1151.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Chan Chen, Yong-mei Meng , Peng Zhang, Juan Wang, Hui-hui Zhao, Shu-zhen Guo , Wei Wang. Diagnosis and treatment rule of traditional Chinese medicine for syndrome factors of chronic congestive heart failure: a study based on Shannon entropy method. Journal of Chinese Integrative Medicine, 2010, 8(11): 1080-1084
[2] Oluwole Busayo Akinola. Should complementary and alternative medicine familiarisation modules be taught in African medical schools?. Journal of Chinese Integrative Medicine, 2011, 9(11): 1165-1169
[3] Xiang-yu Kong, Yuan Hao, Tai-xiang Wu, Yan-ming Xie. Adverse drug reactions or adverse events of Chaihu Injection: A systematic review. Journal of Chinese Integrative Medicine, 2010, 8(12): 1124-1132
[4] Li Tao, Ying-jie Zhu , Xian-mei Lu , Ying Gu , Ai-guang Zhao , Jian Zheng, Chuan-gang Fu, Jin-kun Yang. Clinical study on survival benefit for elderly patients with resected stage I or E colorectal cancer based on traditional Chinese medicine syndrome differentiation and treatment. Journal of Chinese Integrative Medicine, 2010, 8(12): 1159-1164
[5] Yong-mei Liu, Rui-hua Liu, Wen-jun Liu, Li Liu , Zhi-kui Wu, Yu-ying Chen. Effects of Chinese herbal medicine Yiqi Huoxue Formula on TGF-p/smad signal transduction pathway and connective tissue growth factor in rats with renal interstitial fibrosis. Journal of Chinese Integrative Medicine, 2010, 8(12): 1165-1173
[6] Ru-jiang Li, Shu-dong Qiu, Hong-xia Chen, Li-rong Wang. Immunomodulatory effects of Astragalus polysaccharide in diabetic mice. Journal of Chinese Integrative Medicine, 2008, 6(2): 166-170
[7] Jin-yu Xia, Yao-yong Zhou, You-yun Zhao. Relationship between hepatitis B virus in gastric mucosa and syndrome of disharmony between liver and stomach in patients with chronic hepatitis B. Journal of Chinese Integrative Medicine, 2005, 3(1): 19-22
[8] Li Liu, Hong-quan Zhang​. Effects of Danshen Decoction on experimental gastric ulcer in rats and mice. Journal of Chinese Integrative Medicine, 2005, 3(1): 35-38
[9] Cai Rong-lin, Hu Ling, Wu Zi-jian, Wang Ke-ming, He Lu, Zhou Yi-ping, Zhao Lun, Hu Wu-bin. Effects of acupuncture at Shenmen (HT7) or Taiyuan (LU9) on P3a and P3b of event-related potentials in healthy young adults. Journal of Chinese Integrative Medicine, 2012, 10(1): 48-53
[10] Hitender Sharma, Munish Garg. A review of traditional use, phytoconstituents and biological activities of Himalayan yew, Taxus wallichiana. Journal of Integrative Medicine, 2015, 13(2): 80-90