慢性浅表性胃炎不同程度脾胃湿热证与胃黏膜水通道蛋白3、4基因表达的相关性

doi:10.3736/jcim20100203

Abstract

Objective

To explore the relationship between gene expressions of aquaporin (AQP) 3 and 4 and various degrees of spleen-stomach dampness-heat syndrome in chronic superficial gastritis (CSG).
Methods

Twenty-four CSG patients were divided into three groups according to the degrees of spleen-stomach dampness-heat syndrome: mild, moderate and severe groups. There were 8 patients in each group, and another 8 healthy persons were selected as normal control. Samples of mucosa of upper stomach in the included patients and normal persons were collected by gastroscopy, and then were stored in liquid nitrogen. The gene expressions of AQP3 and AQP4 in gastric tissue were examined by fluorescence quantitative polymerase chain reaction.
Results

The gene expressions of AQP3 and AQP4 in the moderate and severe groups were higher than that in the mild group and normal group (P<0.05, P<0.01). The gene expression of AQP3 in the severe group was higher than that in the moderate group (P<0.05).
Conclusion

The gene expressions of AQP3 and AQP4 in gastric mucosa are different in patients with various degrees of spleen-stomach dampness-heat syndrome of CSG. There is a relationship between expressions of AQP3 and AQP4 genes and the degrees of spleen-stomach dampness-heat syndrome, and the gene expressions will increase with the aggravation of the dampness-heat syndrome.

Key words: Gastritis, Aquaporin, Gene expression, Dampness-heat syndrome, Fluorescence quantitative polymerase chain reaction

Wu-xuan Mei, Shao-xian Lao, Na Yu, Zheng Zhou, Lie-ping Huang, Bin Hu. Relationship between gene expressions of aquaporin 3 and 4 and various degrees of spleen-stomach dampness-heat syndrome in chronic superficial gastritis. Journal of Chinese Integrative Medicine, 2010, 8(2): 111-115.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20100203

http://www.jcimjournal.com/EN/Y2010/V8/I2/111

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Group	n	AQP3		AQP4
Group	n	Copy/μg RNA (×10⁴)	Logarithm of copy number	Copy/μg RNA (×10⁴)	Logarithm of copy number
Normal	8	1.263 2±1.642 0	3.849 5±0.507 2	2.802 3±3.787 7	7.038 4±0.670 6
Mild	8	0.711 9±0.456 4	3.884 8±0.442 2	1.827 6±1.954 5	7.045 4±0.501 9
Moderate	8	5.139 0±4.820 1	4.524 5±0.461 7^*△	9.124 8±5.365 2	7.830 7±0.443 8^**△
Severe	8	75.843 9±123.523 7	5.384 9±0.579 4^**△△▲	29.456 3±31.245 3	8.242 6±0.501 3^**△△

Relationship between gene expressions of aquaporin 3 and 4 and various degrees of spleen-stomach dampness-heat syndrome in chronic superficial gastritis

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