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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (5): 517-523.doi: 10.3736/jcim20080517

• Original Experimental Research • Previous Articles     Next Articles

Salvia Miltiorrhiza injection relieves peritoneal dialysis solution-induced injuries of peritoneal structure and function in rats

Dan Zhang, Jing Shu, Yi Wang()   

  1. Department of Nephrology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2008-03-14 Online:2008-05-20 Published:2008-05-15
  • Contact: WANG Yi

Objective: To investigate the effect of Salvia Miltiorrhiza injection (SMI) on peritoneal dialysis solution (PDS) induced injuries of peritoneal structure and function in a rat model, and to observe the relationship between the failure of peritoneal dialysis and expressions of aquaporin-1 (AQP-1) and zonula occluden-1 (ZO-1) in peritoneal tissues.
Methods: Fifty-six SD rats were randomly divided into normal control group, 1.5% PDS group, 4.25% PDS group, 1.5%PDS+1% SMI group, 1.5%PDS+2% SMI group, 4.25% PDS+1% SMI group and 4.25% PDS+2% SMI group. Two-hour peritoneal dialysis test was performed in rats in different groups by intraperitoneal injection for 8-week. Then rats were killed on the 8th week, and the bloods and peritoneal tissues were gathered. The rate of ultrafiltration, clearance rates of urea nitrogen, creatinine and glucose of peritoneum and content of total protein in PDS were detected. Peritoneal membrane histology was evaluated by light microscopy and transmission electron microscopy. Expressions of ZO-1 and AQP-1 proteins in peritoneal tissues were detected by immunohistochemical method, and AQP-1 protein expression was also detected by Western blotting technique.
Results: Compared with normal control group, using of 1.5% PDS and 4.25% PDS caused the changes of structure and function in peritoneum, such as pathological change of peritoneum, decreasing of the rate of ultrafiltration (P<0.05), clearance rates of creatinine and glucose (P<0.01) and the expression of ZO-1 protein (P<0.05), and increasing of the expression of AQP-1 protein (P<0.05). Compared with the simple PDS groups, the pathological damage of peritoneum was lessened and the rate of ultrafiltration and clearance rates of creatinine and glucose were increased in the 1.5% PDS+2% SMI group and 1.5% PDS+2% SMI group. Expression of AQP-1 protein was decreased by 1.5% PDS+2% SMI as compared with 1.5% PDS (P<0.05).
Conclusion: SMI can relieve the injuries of function and structure of peritoneum by down-regulating the expression of AQP-1 protein.

Key words: peritoneal dialysis, activating blood circulation to dissipate blood stasis, Salvia Miltiorrhiza injection, aquaporin-1, zonula occluden-1, rats

CLC Number: 

  • R285.5

Figure 1

Pathological section result of normal control group A: LM, HE staining×100; B: EM×9 900; C: EM×26 500."

Figure 2

Pathological section result of 1.5% PDS group A: LM, HE staining×100; B: EM×9 900; C: EM×26 500."

Figure 3

Pathological section result of 4.25% PDS group A: LM, HE staining×100; B: EM×9 900; C: EM×16 500."

Figure 4

Pathological section result of 1.5% PDS+2% SMI group A: LM, HE staining×100; B: EM×9 900; C: EM×20 500."

Table 1

Volume of UF and concentration of total protein in PDS ($\bar{x}$±s)"

Group n UF (ml) Content of total protein (mg/ml)
Normal control 8 20.63±3.38 2.47±0.77
1.5% PDS 8 14.38±4.24* 1.63±0.05*
4.25% PDS 7 14.57±6.13* 1.90±0.18*
1.5% PDS+2% SMI 8 17.00±4.04 2.75±0.68△△
1.5% PDS+1% SMI 8 11.00±7.45*■ 2.58±0.45△△
4.25% PDS+2% SMI 7 10.71±8.36*■ 2.33±0.58
4.25% PDS+1% SMI 7 10.00±4.47* 2.05±0.07

Table 2

Clearance rates of urea nitrogen, creatinine, glucose by peritoneum ($\bar{x}$±s, ml/min)"

Group n Clearance rate of urea nitrogen Clearance rate of creatinine Clearance rate of glucose
Normal control 8 0.18±0.03 0.16±0.05 4.04±1.42
1.5% PDS 8 0.23±0.04** 0.16±0.04 0.99±0.42**
4.25% PDS 6 0.21±0.05 0.16±0.04 1.00±0.25**
1.5% PDS+2% SMI 7 0.22±0.05 0.20±0.04△△ 1.79±0.77**
1.5% PDS+1% SMI 7 0.21±0.04 0.17±0.04 1.68±0.27**
4.25% PDS+2% SMI 7 0.25±0.05** 0.26±0.04**▲▲□ 2.01±0.05**▲▲
4.25% PDS+1% SMI 6 0.22±0.03 0.17±0.01 1.42±0.16**
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