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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (4): 342-348.doi: 10.3736/jcim20090408

• Original Experimental Research • Previous Articles     Next Articles

Effects of berberine on differentiation and bone resorption of osteoclasts derived from rat bone marrow cells

WEI Peng,JIAO Lei,QIN Lu-ping,YAN Fei,HAN Ting,ZHANG Qiao-yan()   

  1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received:2008-10-30 Accepted:2009-01-02 Online:2009-04-20 Published:2018-10-15

Objective: To observe the effects of berberine on osteoclastic differentiation and bone resorption action in vitro, and to investigate the cellular mechanism of its inhibitory effects on bone resorption.
Methods: The multinucleated osteoclasts (MNCs) were derived by 1,25-dihydroxyvitamin D3 and dexamethasone from bone marrow cells in the coculture system with primary osteoblastic cells. The tartrate-resistant acid phosphatase (TRAP) staining and image analysis of bone resorption pit on dental slices were used to identify osteoclast. The activity of TRAP was measured by p-nitrophenyl sodium phosphate assay. The bone resorption pit area on the bone slices formed by osteoclasts was measured by computer image processing.
Results: At the concentrations of 0.1, 1 and 10 μmol/L, berberine dose-dependently suppressed the formation of TRAP-positive multinucleated cells, the TRAP activity and the osteoclastic bone resorption. The strongest inhibitory effect was exhibited at the concentration of 10 μmol/L, with the inhibiting rate of 60.45%, 42.12% and 72.69% respectively.
Conclusion: Berberine can decrease bone loss through inhibition of osteoclast formation, differentiation and bone resorption.

Key words: berberine, osteoporosis, osteoclasts, bone resorption

Figure 1

Osteoclasts stained by TRAP (Inverted phase contrast microscopy, ×400)"

Figure 2

Bone resorption pits formed by osteoclasts (Inverted phase contrast microscopy, ×100) A: Control; B: Dental slice cocultured with osteoclasts treated with 10 μmol/L berberine."

Figure 3

Osteoclast differentiation in morphology (Inverted phase contrast microscopy, ×200) A: Control, normal mature osteoclasts; B: Osteoclasts treated with berberine at 10 μmol/L."

Figure 4

Inhibitory effect of berberine on formation of the TRAP-positive multinucleated osteoclasts **P<0.01, vs control group. Data represent as x±s, n=15."

Figure 5

Inhibitory effect of berberine on the osteoclastic TRAP activity **P<0.01, vs control group. Data represent as x±s, n=15."

Figure 6

Inhibitory effect of berberine on bone resorption pit area formed by osteoclasts **P<0.01, vs control group. Data represent as x±s, n=15."

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