红藤颗粒剂对大鼠异位子宫内膜血管内皮生长因子及其受体胎肝激酶-1表达的影响

doi:10.3736/jcim20090411

Abstract

Objective

To investigate the effects of Caulis Sargentodoxae Granule (CSG), a compound traditional Chinese herbal medicine for treating endometriosis, on expressions of vascular endothelial growth factor (VEGF) and its receptor-2 fetal liver kinase-1 (Flk-1) in rats with endometriosis.
Methods

A rat model of endometriosis was established by autotransplant of endometria. Forty-eight endometriosis rats were randomly divided into 6 groups: untreated group, castrate group, mifepristone group (5 mg/kg) and low- (20 g/kg), medium- (40 g/kg) and high-dose (80 g/kg) CSG group. After 21-day consecutive treatment, the expressions of VEGF and its receptor Flk-1 in ectopic endometria were detected by immunohistochemical method, and the volume of the ectopic endometria was also measured by using electronic digital calipers.
Results

Compared with the untreated group, the endometrial volumes in the treated groups were significantly decreased (P<0.05) except for the low-dose CSG group. Histopathologic observation showed that the epithelial layer of ectopic endometrium was less and the thickness of epithelial layer was thinner than those in the untreated group (P<0.01), but the low-dose CSG group had few changes. The VEGF and its receptor Flk-1 expressed abundantly in the cytoplasm of the glandular epithelium and vascular endothelial cell in all the groups. Compared with the untreated group, the expressions of VEGF and Flk-1 of ectopic endometrium in the CSG-treated groups, the mifepristone group and the castrate group were significantly decreased (P<0.05, P<0.01).
Conclusion

CSG can treat the endometriosis in rats, and its action may be associated with decreasing the expressions of VEGF and its receptor Flk-1 in ectopic endometrium.

Key words: Caul is Sargentodoxae Granules, Endometriosis, Vascular endothelial growth factor, Vascular endothelial growth factor receptor-2, Rats

Yang Cao, Ting-ting Zhang, Shu-wu Xie, Yan Zhu, Lan-di Shu, Zhi-li Song, Lin Cao, De-ying Dai. Effects of Caulis Sargentodoxae Granule on expressions of vascular endothelial growth factor and its receptor-2 in rats with endometriosis. Journal of Chinese Integrative Medicine, 2009, 7(4): 400-400.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20090411

http://www.jcimjournal.com/EN/Y2009/V7/I4/400

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Figures/Tables 6

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References 10

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Group	n	Volume of ectopic endometrium (mm³)		Inhibition rate (%)
Group	n	Before treated	After treated	Inhibition rate (%)
Untreated	8	71.958 (52.504, 89.750)	89.012 (49.598, 235.497)	–21.582(–310.009, 20.914)
Castrate	8	73.215 (43.896, 130.233)	1.769 (0.001, 23.977)*	99.211 (65.506, 99.996)*
Mifepristone	8	105.096 (84.601, 115.745)	36.340 (25.642, 48.712)*	60.791 (40.695, 67.034)*
Low-dose CSG	8	51.703 (44.215, 66.761)	33.242 (24.758, 53.559)	49.471 (–40.899, 69.698)
Medium-dose CSG	8	65.582 (40.893, 86.315)	24.104 (17.723, 36.332)*	54.407 (20.225, 75.781)*
High-dose CSG	8	64.529 (54.367, 109.411)	18.439 (8.967, 36.464)*	75.759 (56.734, 85.050)*

Group	n	Thickness of ectopic endometrium
Untreated	8	317.44±63.38
Castrate	8	112.39±19.09**
Mifepristone	8	182.50±23.06**
Low-dose CSG	8	272.50±62.78
Medium-dose CSG	8	200.46±32.89**
High-dose CSG	8	128.20±38.39**

Group	n	VEGF	Flk-1
Untreated	8	90.81±11.45	98.78±8.56
Castrate	8	112.08±4.10**	124.22±4.20**
Mifepristone	8	106.67±1.37*	123.97±15.44**
Low-dose CSG	8	97.69±4.48	105.94±8.19
Medium-dose CSG	8	102.64±2.80*	115.00±12.28*
High-dose CSG	8	109.06±2.73**	122.86±21.22*

Effects of Caulis Sargentodoxae Granule on expressions of vascular endothelial growth factor and its receptor-2 in rats with endometriosis

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