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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (9): 1049-1055.doi: 10.3736/jcim20120914

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Effects of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in Wistar rats

Lawrence A. Olatunji1(),Taofeek O. Usman1, Joseph O. Adebayo2, Victoria A. Olatunji3   

  1. 1. Department of Physiology, College of Health Sciences, University of Ilorin, Ilorin 240001, Nigeria
    2. Department of Biochemistry, Faculty of Science, University of Ilorin, Ilorin 240001, Nigeria
    3. Department of Ophthalmology, University of Ilorin Teaching Hospital, Ilorin 240001, Nigeria
  • Received:2012-02-17 Accepted:2012-03-31 Online:2012-09-20 Published:2018-09-15
  • Contact: A. Olatunji Lawrence

Objective: To investigate the effects of oral administration of aqueous extract of Hibiscus sabdariffa on renal Na +-K +-ATPase and Ca 2+-Mg 2+-ATPase activities in rats.

Methods: The 25 and 50 mg/(kg·d) of aqueous extracts of H. sabdariffa were respectively given to rats in the experimental groups for 28 d, and rats in the control group received an appropriate volume of distilled water as vehicle. Na +-K +-ATPase and Ca 2+-Mg 2+-ATPase activities in the kidney were assayed by spectrophotometric method.

Results: Administrations of 25 and 50 mg/(kg·d) of aqueous extract of H. sabdariffa significantly decreased the Ca 2+-Mg 2+-ATPase activity in the kidney of rats (P<0.05). However, the renal Na +-K +-ATPase activity of the experimental rats was not affected by either dose of the extract. And the plasma Na +, K + and Ca 2+ levels of the experimental rats had no significant changes. Administration of either dose of the extract did not result in any significant changes in body and kidney weights, the concentrations of plasma albumin and total protein, and alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase activities. However, concentrations of creatinine and urea were significantly reduced by 50 mg/kg of the extract (P<0.05).

Conclusion: The present study indicates that oral administration of aqueous extract of H. sabdariffa may preserve the renal function despite a decreased renal Ca 2+-Mg 2+-ATPase activity. KEYWORD: Hibiscus; Ca 2+-Mg 2+-ATPase; sodium-potassium-exchanging ATPase; kidney function tests; rats

Key words: Hibiscus, Ca2+-Mg2+-ATPase, sodium-potassium-exchanging ATPase, kidney function tests, rats


Group n Body weight (g) Ratio of kidney weight to body weight
Control 6 178.2±12.2 0.61±0.05
Extract of Hibiscus sabdariffa (25 mg/kg) 6 181.3±9.7 0.64±0.02
Extract of Hibiscus sabdariffa (50 mg/kg) 6 180.8±11.4 0.65±0.04


Control 6 208.5±15.5 87.5±10.1 10.4±1.4
Extract of Hibiscus sabdariffa (25 mg/kg) 6 212.6±13.1 101.3±13.0 13.1±4.1
Extract of Hibiscus sabdariffa (50 mg/kg) 6 221.4±20.2 95.2±11.4 12.2±2.4

Figure 1

Effects of aqueous extract of Hibiscus sabdariffa on Ca2+ -Mg2+-ATPase activity in rat kidneyData are presented as mean ± standard error of mean, n=6; *P<0.05, vs control group. "

Figure 2

Effects of aqueous extract of Hibiscus sabdariffa on Na+ -K+-ATPase activity in rat kidneyData are presented as mean ± standard error of mean, n=6. "


Group n Sodium Potassium Calcium
Control 6 142.2±1.2 5.0±0.1 2.4±0.1
Extract of Hibiscus sabdariffa (25 mg/kg) 6
Extract of Hibiscus sabdariffa (50 mg/kg) 6


Group n Plasma total protein (g/L) Albumin (g/L) Creatinine (mg/L) Urea (mg/L)
Control 6 6.48±0.06 3.65±0.07 6.05±0.20 0.47±0.01
Extract of Hibiscus sabdariffa (25 mg/kg) 6 6.78±0.11 3.83±0.12 5.95±0.38 0.46±0.03
Extract of Hibiscus sabdariffa (50 mg/kg) 6 6.62±0.06 3.64±0.06 4.89±0.12* 0.37±0.03*
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