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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (5): 372-374.doi: 10.3736/jcim20040518

• Technique and Methods • Previous Articles     Next Articles

Establishment and biological characteristics of orthotopic transplantation tumor model of hepatocellular carcinoma in mice

Ke-qi Han(), Wei Gu, Xia Hu, Ya-ni Zhang, Xue-qiang Huang, Ling Liu, Yong-hua Su, Chang-quan Ling   

  1. Department of Tranditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2004-05-31 Online:2004-09-20 Published:2018-10-20

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Objective: To study the feasibility of the establishment of the orthotopic transplantation tumor model of hepatocellular carcinoma in mice and its tumor biological characteristics.Methods: H22 cells of hepatocellular carcinoma were inoculated to form ectopic transplanted model in mice by subcutaneous injection. Then the subcutaneous tumors were implanted into the liver of mice, and the orthotopic transplantation tumor model of hepatocellular carcinoma was established.Results: The successful rate of the orthotopic transplantation tumor model was 95.6% and the spontaneous metastatic rate was 81.8%, the rate of mass ascites was 40.9% and the natural extinctive rate was 0%. The natural survival time in the orthotopic transplantation tumor model was 28 days and the proliferation of tumor in transplanted model was accelerated after 2 weeks or so.Conclusion: The orthotopic transplantation tumor model in mice is an ideal model for studying the metastatic mechanism and screening anti-tumor drugs for liver cancer, just because of its high successful rate and high spontaneous metastatic rate with no natural extinction.

Key words: hepatoma, metastasis, neoplasm, mice, experimental animal models

CLC Number: 

  • R735.7

Fig 1

Orthotopic transplantation tumor model of hepatocellular carcinoma in ICR mice on the 14 th day."

Fig 2

Orthotopic transplantation tumor model of hepatocellular carcinoma in ICR mice on the 35th day with extensive implantation and retroperitoneal lymph node metastasis."

Fig 3

Pathological changes in orthotopic transplantation tumor in ICR mice （HE staining,×40）"

Fig 4

Ultrastructural changes in orthotopic transplantation tumor in ICR mice （×9 000）"

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