Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (5): 375-378.doi: 10.3736/jcim20040519

• Technique and Methods • Previous Articles     Next Articles

Osteogenesis characteristics of cultured rat mesenchymal stem cells under bone induction condition

Dong-feng Chen1(), Hui Li1, Jian-hong Zhou1, Yao Xie2, Yi-wei Li1, Shao-hui Du3, Yi Zhang4, Jie Huang5, Min Xu6   

  1. 1. Department of Anatomy , Guangzhou University of Traditional Chinese Medicine , Guangzhou , Guangdong Province 510405 , China
    2. Department of Anatomy , Zhongshan Medical College, SUN Yat-sen University , Guangzhou , Guangdong Province 510080 ,China
    3. Department of Internal Medicine, Shenzhen Hospital of Traditional Chinese Medicine , Shenzhen ,Guangdong Province 518000 , China
    4. Shenzhen Shahe Hospital , Shenzhen , Guangdong Province 518000 , China
    5. Shenzhen People’s Hos pital , Shenzhen , Guangdong Province 518000 , China ;
    6. School of Traditional Chinese Medicine , HongKong Baptise Univesity , Hong Kong , China
  • Received:2003-09-20 Online:2004-09-20 Published:2018-10-20

Objective: To investigate the osteogenesis characteristics of cultured rat mesenchymal stem cells (MSCs) under bone induction condition.Methods: MSCs were isolated from adult rat by using density gradient separation method. The osteogenic inducers were compounds of Dexone, β-glycerophosphate sodium and vitamin C.Results: The MSC attachment formed soon after the seeding and grew into colonies with the appearance of fibroblastic cells. The osteogenic inducer with low dose of Dexone could promote the osteogenic differentiation of MSC.In the group of osteogenic inducer with low dose of Dexone, the expression of alkaline phosphatase (ALP) was remarkably increased after one week’s induction, and the number of positive cells was (15.1±2.6), significantly higher than that of the control group (12.0±3.5) (P<0.01). The calcified deposits began to appear in the group of osteogenic inducer with low dose of Dexone after one week's induction and was increased remarkably after three weeks, and the number of calcified deposits was (9.0±1.7), significantly higher than that of the control group (2.0±1.8) (P<0.01).Conclusion: MSC can differentiate into osteogenesis by osteogenic induction and may be used to provide seed cells for bone tissue engineering.

Key words: stem cell myeloid, osteoblasts, animal, laboratory

CLC Number: 

  • R329.471

Fig 1

Primary MSCs culture with appearance of colony-forming units-fibroblast (×200)"

Fig 2

Passage 1 MSCs showing fibroblast-like array (×100)"

Tab 1

Number of ALP positive cells after induction($\bar{x}$±s,cells)"

Group n 7 d 14 d 21 d 28 d
Control 20 12.0±3.5 13.3±3.2 14.6±3.9 4.5±3.1
Low dose 20 15.1±2.6** 16.6±2.3** 18.4±2.2** 19.4±2.5**
High dose 20 13.2±1.9 14.7±2.5 15.7±2.6 16.1±2.3

Fig 3

ALP positive cells in passage 1 MSCs after 7 d low dose Dexone induction (×200)"

Tab 2

Number of calcified deposits after induction($\bar{x}$±s,deposits)"

Group n 7 d 14 d 21 d 28 d
Control 10 0 2.1±1.4 2.0±1.8 3.5±1.7
Low dose 10 4.3±1.6** 7.6±1.6** 9.0±1.7** 9.3±1.4**
High dose 10 0 1.9±1.3 3.5±1.4 4.7±1.5

Fig 4

Calcified deposits in passage 1 MSCs after 7d low dose Dexone induction (×200)"

[1] Chen D, Ji X, Harris MA , et al. Differential roles for bone morphogenetic protein(BMP)receptor type IB and IA in differentiation and specification of mesenchymal precursor cells to osteoblast and adipocyte lineages[J]. J Cell Biol, 1998,142(1):295-305
doi: 10.1083/jcb.142.1.295
[2] Jaiswal N, Haynesworth SE, Caplan AI , et al. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro[J]. J Cell Biochem, 1997,64(2):295-312
doi: 10.1002/(ISSN)1097-4644
[3] Coelho MJ, Fernandes MH . Human bone cell cultures in biocompatibility testing.PartⅡ:effect of ascorbic acid, beta-glycerophosphate and dexamethasone on osteoblastic differentiation[J]. Biomaterials, 2000,21(11):1095-1102
doi: 10.1016/S0142-9612(99)00192-1
[4] Pittenger MF, Mackay AM, Beck SC , et al. Multilineage potential of adult human mesenchymal stem cells[J]. Science,1999, 284(5411):143-147
doi: 10.1126/science.284.5411.143 pmid: 10102814
[1] Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
[2] Arthur Yin Fan, Sarah Faggert . Dr. Gene Bruno: The beginning of the acupuncture profession in the United States (1969-1979) — Acupuncture, medical acupuncture and animal acupuncture. Journal of Integrative Medicine, 2015, 13(5): 281-288.
[3] Vamsi Reddy, Arvind Sridhar, Roberto F. Machado, Jiwang Chen. High sodium causes hypertension: Evidence from clinical trials and animal experiments. Journal of Integrative Medicine, 2015, 13(1): 1-8.
[4] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[5] Su-fang Zhang, Chang-quan Ling , Bai Li, Hong-yun Chen , Zhe Chen. Effects of Sisheng Decoction on the immunity and anti-stress function in mice with spleen deficiency syndrome. Journal of Chinese Integrative Medicine, 2012, 10(12): 1465-1469.
[6] You-hua Wang , Shang Li , Bin-rui Yang , Lun-qing Zhang , Xin Zhou , Bei-wen Xu, Ming-yuan Li . Use of zebrafish models for the research of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1189-1197.
[7] Hui Han , Li-min Wu , Ming-xiang Han, Wen-ming Yang , Dao-jun Xie , Zhao-hui Fang , Dan-chen Chu , Fang Fang . Effects of Chinese herbal medicine Shenxiong Yujing Granule on regeneration of neural cells in rats with diabetes-associated cerebral ischemia. Journal of Chinese Integrative Medicine, 2012, 10(10): 1140-1148.
[8] Liu Yue, Xie Ming, Zhang Ye. Dynamic changes of laboratory parameters of rats with type 2 diabetes and insulin resistance: defining their role in development of traditional Chinese medicine syndrome. Journal of Chinese Integrative Medicine, 2012, 10(1): 100-108.
[9] Qian Zhao, Rui Jin, Bing Zhang, Sen-mao Liu, Min Li, Xin Liu, Lian-zhen Li, Qian Zhang . Ridit analysis of experimental data from animal models of yang deficiency induced by different doses of hydrocortisone. Journal of Chinese Integrative Medicine, 2011, 9(9): 941-947.
[10] Chen Wang, Jin-gen Lu, Hao-qiang Yin, Yong-qing Cao. Thread-dragging therapy in treatment of a rat model of infected subcutaneous fistula. Journal of Chinese Integrative Medicine, 2011, 9(5): 565-569.
[11] Cai Lin-lin, Li Hao, Liu Jian-gang, Liu Long-tao, Guan Jie, Liu Ming-fang, Hu Jia, Wei Yun. Effects of early intervention with Huannao Yicong formula effective components on behavior and cholinergic system of β-amyloid precursor protein transgenic mice. Journal of Chinese Integrative Medicine, 2011, 9(3): 292-298.
[12] Yong-chang Diwu, Jin-zhou Tian, Jing Shi. Effects of Chinese herbal medicine Yinsiwei compound on spatial learning and memory ability and the ultrastructure of hippocampal neurons in a rat model of sporadic Alzheimer disease. Journal of Chinese Integrative Medicine, 2011, 9(2): 209-215.
[13] Srinivas Ashok Kumar Bagepalli, Lakshman Kuruba, Avalakondarayppa Arun Kumar Peresandra, Lakshminarayana Shastry Viswantha Gollapalle, Prabhakar Veerapur Veeresh, Shivanadppa Thippeswamy Boreddy, Manoj Bachappa. Hepatoprotective activity of methanol extract of Amaranthus caudatus Linn. against paracetamol-induced hepatic injury in rats. Journal of Chinese Integrative Medicine, 2011, 9(2): 194-200.
[14] Yan-yan Tao, Qing-lan Wang, Ji-li Yuan, Li Shen, Cheng-hai Liu. Effects of vitamin E on mercuric chloride-induced renal interstitial fibrosis in rats and the antioxidative mechanism. Journal of Chinese Integrative Medicine, 2011, 9(2): 201-208.
[15] Qin Bian, Jian-hua Huang, Zhu Yang, You Ning, Yong-jian Zhao, Yong-jun Wang, Zi-yin Shen. Effects of active ingredients in three kidney-tonifying Chinese herbal drugs on gene expression profile of bone marrow stromal cells from a rat model of corticosterone-induced osteoporosis. Journal of Chinese Integrative Medicine, 2011, 9(2): 179-185.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239