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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (3): 220-223.doi: 10.3736/jcim20050315

• Original Experimental Research • Previous Articles     Next Articles

Effects of Wenshen Jianpi Recipe on chronic wound healing in rats

Yong-qing Cao,Chun-mei He,Jin-gen Lu   

  1. Shanghai Municipal Clinical Medicine Center for Chinese Traditional Surgery, Shanghai 200032, China
  • Received:2005-03-29 Online:2005-05-20 Published:2005-05-20
  • Contact: Yong-qing Cao E-mail:CaoYQ88@sh163.net

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Objective

To study the effects of Wenshen Jianpi Recipe (WSJPR, a traditional Chinese medicine for warming kidney and invigorating spleen) on chronic wound healing and the mechanism.

Methods

Ninety-six SD rats were randomly divided into 4 groups, with 24 rats in each group, and back wound was made in the rats. For rats in 3 of the 4 groups, hydrocortisone injection was administered to induce chronic wound. Rats in 2 of the 3 groups were treated with WSJPR and Xinpukang Granules (XPKG) respectively, and the rats in the other group were untreated. The rats in the fourth group were taken as control. The wound healing time and the width of new epidermis were observed, and the histomorphological changes and cell cycle of the granulation tissue, and the protein expressions of epidermal growth factor (EGF), transforming growth factor-β1 (TGF-β1) and fibronectin (FN) in the granulation tissue were tested with immunohistochemical technique and flow cytometry.

Results

The wound healing time of the WSJPR-treated and XPKG-treated groups was (17.0±1.9) and (18.8±1.9) d respectively, much shorter than that of the untreated and control groups (P<0.05). On the 14th experiment day, the width of new epidermis of the WSJPR-treated and XPKG-treated groups was (3.73±0.19) and (3.21±0.15) mm respectively, much wider than that of the untreated and control groups (P<0.05). The numbers of angiogenesis, fibroblasts and cells in the S phase in WSJPR-treated and XPKG-treated groups were much higher than those in the untreated and control groups (P<0.05). Compared with the untreated and control groups, the protein expressions of EGF, TGF-β1 and FN in WSJPR-treated and XPKG-treated groups were higher (P<0.05).

Conclusion

WSJPR can enhance the wound healing. It was likely through accelerating the cell proliferation and up-regulating the expressions of EGF, TGF-β1 and FN.

Key words: Pharmacotherapy, TCM, Wound healing, Granulation tissue, Histology, Comparative, Cytokines

CLC Number: 

  • R283.661

Tab 1

Wound healing time and width of new epidermis on the 14th experiment day （$\bar{x}$±s）"

Group n Wound healing time (d) Width of new epidermis (mm)
Untreated group 8 22.4±2.1 1.96±0.14
Control group 8 20.1±1.8 2.64±0.21
WSJPR-treated group 8 17.0±1.9*△ 3.73±0.19*△
XPKG-treated group 8 18.8±1.9*△ 3.21±0.15*△

Tab 2

Scores of histomorphological examination of granulation tissue（$\bar{x}$±s）"

Group n Angiogenesis Fibroblast Necrosis
Untreated group 6 1.50±0.55 1.33±0.52 1.83±0.75
Control group 6 1.67±0.82 1.67±0.82 1.67±0.52
WSJPR-treated group 6 2.33±0.52*△▲ 2.50±0.55*△▲ 1.16±0.75
XPKG-treated group 6 1.83±0.75 1.83±0.75 1.50±0.55

Tab 3

Cells in different cell cycles of granulation tissue（$\bar{x}$±s, %）"

Group n G0-G1 G2-M S
Untreated group 8 84.9±2.6 3.6±1.1 11.5±1.9
Control group 8 82.6±1.7 3.8±0.8 13.6±2.1
WSJPR-treated group 8 82.1±2.8 3.5±0.5 14.6±2.9*△▲
XPKG-treated group 8 82.6±2.1 3.2±1.1 14.2±1.2

Tab 4

Protein expressions of EGF, TGF-β1 and FN in granulation tissue （$\bar{x}$±s）"

Group n IOD (×103)
EGF TGF-β1 FN
Untreated group 4 61.28±8.95 62.26±2.85 121.03±35.69
Control group 4 86.42±11.70 268.53±107.20 284.00±66.88
WSJPR-treated group 4 179.47±35.56*△ 452.64±56.32*△ 482.57±16.91*△
XPKG-treated group 4 106.08±28.09*△ 281.33±20.04 339.96±60.19*△
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