[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (5): 378-381.doi: 10.3736/jcim20050512

• Original Experimental Research • Previous Articles     Next Articles

Growth-inhibiting and anti-metastasis effects of Weichang'an Decoction on orthotopic transplant nude mouse model of human gastric cancer

Hai-lei Zhao1,Ai-guang Zhao1,Sheng-fu You2,Ying Gu1,Lai-di Tang2,Jin-kun Yang1   

  1. 1. Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Science and Research Laboratory, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2005-04-25 Online:2005-09-20 Published:2005-09-20
  • Contact: Ai-guang Zhao E-mail:aiguang@hotmail.com

RichHTML

1

PDF (PC)

358

Like

Objective

To evaluate the growth-inhibiting and anti-metastasis effects of Weichang'an Decoction (WCAD) on orthotopic transplant nude mouse model of human gastric cancer.

Methods

Forty-one nude mice were implanted with SGC-7901 cells at orthotopic site, whereas 25 were implanted with SGC-7901 cells subcutaneously. Then the nude mice in each transplantation model were divided into the same three groups which were WCAD-treated group with WCAD 0.5 ml/d taken orally, 5-FU-treated group with 5-FU 50 mg/kg intraperitoneally injected weekly and untreated control group with physiological saline 0.5 ml/d taken orally. The growth-inhibiting rates of transplant tumors were detected and the metastatic lesions were examined in the orthotopic transplant mouse model while PCNA-positive rate and apoptotic index (AI) were observed in the subcutaneous transplant mouse model.

Results

The growth-inhibiting rates in the WCAD-treated and 5-FU-treated groups of orthotopic transplant mouse model were 40.82% and 37.92% respectively whereas those of subcutaneous transplant mouse model were 48.70% and 60.10%. The incidence rates of metastasis in perigastric lymph notes, lymph nodes in the porta hepatis, liver, diaphragm and peritoneum in the WCAD-treated and 5-FU-treated groups were lower than those in the untreated control group, and the total metastasis rates in the WCAD-treated, 5-FU-treated and the untreated control groups were 30.77%, 28.57% and 71.43% respectively with significant differences (P<0.05). The total PCNA-positive rates in the WCAD-treated and 5-FU-treated groups were obviously lower than that in the untreated control group (P<0.05 or P<0.01) while the AI was higher than that in the untreated control group (P<0.01). The growth-inhibiting rate, total PCNA-positive rate and total metastasis rate in the WCAD-treated group had no significant differences as compared with those in the 5-FU-treated group, but the AI in the WCAD-treated group was significantly higher than that in the 5-FU-treated group (P<0.05).

Conclusion

WCAD has the inhibiting effects on tumor growth and metastasis of gastric cancer which is orthotopic implanted onto nude mice. This effect may be obtained by proliferation suppression and apoptosis induction in cancer.

Key words: Stomach neoplasms, Compounds (TCD), Neoplasm transplantation, Apoptosis

CLC Number: 

  • R286.91

Tab 1

Tumor metastasis of orthotopic transplant nude mouse model of human gastric cancer in 3 groups （%）"

Group n Perigastric
lymph nodes		 Liver Lymph nodes in the
porta hepatis		 Diaphragm Peritoneum Total metastasis
rate
WCAD-treated 13 0 7.69 (1/13) 0 7.69(1/13) 15.38 (2/13) 30.77(4/13)*
5-FU-treated 14 0 21.43 (3/14) 0 0 14.29 (2/14) 28.57(4/14)*
Untreated control 14 7.14(1/14) 50 (7/14) 28.57 (4/14) 28.57 (4/14) 42.86 (6/14) 71.43 (10/14)

Tab 2

Expression of PCNA and apoptotic index in subcutaneous transplant nude mouse model of human gastric cancer in 3 groups （$\bar{x}$±s, %）"

Group n Total PCNA-
positive rate		 Apoptotic index
WCAD-treated 8 39.03±7.37** 9.72±4.51**△
5-FU-treated 9 43.48±19.77* 5.74±1.75**
Untreated control 8 62.26±13.80 2.45±1.37
[1] 李佩文 . 中医药对维护肿瘤患者生活质量的作用[J]. 中华肿瘤杂志, 2002,24(2):203-204
[2] 王坤根 . 中医辨证治疗胃癌93例[J]. 中医杂志, 2004,45(6):441-442
[3] 邱佳信, 贾筠生, 杨金坤 , 等. 健脾法为主治疗晚期胃癌的探讨[J]. 中医杂志, 1992,33(8):23-25
[4] 赵爱光, 杨金坤, 赵海磊 . 健脾中药诱导人胃癌细胞裸小鼠移植瘤细胞凋亡的实验研究[J]. 世界华人消化杂志, 2000,8(7):737-740
doi: 10.3969/j.issn.1009-3079.2000.07.004
[5] 赵爱光, 赵海磊, 杨金坤 , 等. 人胃癌原位移植模型中宿主脾虚证的探讨[J]. 中国中西医结合消化杂志, 2001,9(4):198-200
doi: 10.3969/j.issn.1671-038X.2001.04.002
[6] 王立生, 潘令嘉, 郑跃杰 , 等 .双歧杆菌对裸鼠腹腔巨噬细胞分泌IL-1以及IL-2的影响[J].中国肿瘤生物治疗杂志, 1998, 5(3): 214, 203
[7] Zhao AG, Zhao HL, Jin XJ , et al. Effects of Chinese Jianpi herbs on cell apoptosis and related gene expression in human gastric cancer grafted onto nude mice[J]. World J Gastroentrerol, 2002,8(5):792-796
doi: 10.3748/wjg.v8.i5.792
[8] 赵怡, 张学庸, 史宪杰 , 等. 胃癌组织p16, p53蛋白和增殖细胞核抗原的表达意义[J]. 世界华人消化杂志, 1999,7(3):246-248
doi: 10.3969/j.issn.1009-3079.1999.03.021
[1] Avijit Paul, Kausik Bishayee, Samrat Ghosh, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Chelidonine isolated from ethanolic extract of Chelidonium majus promotes apoptosis in HeLa cells through p38-p53 and PI3K/AKT signalling pathways. Journal of Chinese Integrative Medicine, 2012, 10(9): 1025-1038.
[2] Yogesh Bendale, Vineeta Bendale, Saili Paul, Soumya Sundar Bhattacharyya. Green synthesis, characterization and anticancer potential of platinum nanoparticles Bioplatin. Journal of Chinese Integrative Medicine, 2012, 10(6): 681-689.
[3] Samadder Asmita, Das Jayeeta, Das Sreemanti, Das Durba, De Arnab, Bhadra Kakali, Rahman Khuda-Bukhsh Anisur. Dihydroxy-isosteviol methyl ester of Pulsatilla nigricans extract reduces arsenic-induced DNA damage in testis cells of male mice: Its toxicity, drug-DNA interaction and signaling cascades. Journal of Chinese Integrative Medicine, 2012, 10(12): 1433-1442.
[4] Min Zhu , Mi Zhou , Ying Shi , Wen-wei Li. Effects of echinacoside on MPP+-induced mitochondrial fragmentation, mitophagy and cell apoptosis in SH-SY5Y cells. Journal of Chinese Integrative Medicine, 2012, 10(12): 1427-1432.
[5] Mukherjee Avinaba, Sikdar Sourav, Bishayee Kausik, Paul Avijit, Ghosh Samrat, Boujedaini Naoual, Rahman Khuda-Bukhsh Anisur. Ethanolic extract of Thuja occidentalis blocks proliferation of A549 cells and induces apoptosis in vitro. Journal of Chinese Integrative Medicine, 2012, 10(12): 1451-1459.
[6] Xiao-ping Huang , Xiao-dan Liu , Chang-qing Deng . Effects of the combination of active component extracts from Astragalus membranaceus and Panax notoginseng on apoptosis, reactive oxygen species and mitochondrial membrane potential of PC12 cells with oxidative injury. Journal of Chinese Integrative Medicine, 2012, 10(10): 1127-1134.
[7] Agung E. Nugroho, Adam Hermawan, Dyaningtyas D. P. Putri, E. dY Meiyanto, Lukman Hakim . Synergistic effects of ethyl acetate fraction of Ficus septica Burm. f. and doxorubicin chemotherapy on T47D human breast cancer cell line. Journal of Chinese Integrative Medicine, 2012, 10(10): 1162-1170.
[8] Hao Dai, Jun Gu, Ling-zhi Li, Li-mei Yang, Hui Liu, Jian-yu Li. Scutellarin benzyl ester partially secured the ischemic injury by its anti-apoptosis mechanism in cardiomyocytes of neonatal rats. Journal of Chinese Integrative Medicine, 2011, 9(9): 1014-1021.
[9] Ci-an Zhang, Feng Wu, Zhu-jun Mao, Zhen Wei, Yong-jin Li, Pin-kang Wei. Effects of ethanol extract of Rhizome Pinelliae Preparata on intracellular pH value of human gastric adenocarcinoma cells. Journal of Chinese Integrative Medicine, 2011, 9(8): 894-900.
[10] Yu Miao, Yue-rong Jiang, Lin Yang, Cheng-dong Xia, Lu Zhang, Cai-feng Wu, Da-zhuo Shi, Hui-jun Yin, Ke-ji Chen. Effects of Chinese herbal drug-containing serum on oxidative damage and apoptosis of umbilical vein endothelial cells induced by oxidized low-density lipoprotein. Journal of Chinese Integrative Medicine, 2011, 9(5): 539-545.
[11] Zhao-hui Chu, Xiao-hua Liang, Xin-li Zhou, Ruo-fan Huang, Qiong Zhan, Jing-wei Jiang. Effects of deguelin on proliferation and apoptosis of MCF-7 breast cancer cells by phosphatidylinositol 3-kinase/Akt signaling pathway. Journal of Chinese Integrative Medicine, 2011, 9(5): 533-538.
[12] Bo Yang, Xue-chun Lu, Feng Zhang, Yang Liu, Hong-li Zhu. Inhibitory effects of traditional Chinese herbal medicine Tanreqing injection on proliferation of leukemia cells in vitro and the potential mechanisms. Journal of Chinese Integrative Medicine, 2011, 9(4): 414-422.
[13] Shu-qiang Wang, Wu Rao, Lian-fu Deng, Ya-ping Zhu, Yong-qiang Chen. Effects of rat serum containing Chinese herbal medicine Sangen Decoction on osteoclastogenesis and bone resorption of osteoclasts induced by polymethylmethacrylate particles. Journal of Chinese Integrative Medicine, 2011, 9(1): 64-69.
[14] Khuda-Bukhsh AR, Bhattacharyya SS, Paul S, Boujedaini N. Polymeric nanoparticle encapsulation of a naturally occurring plant scopoletin and its effects on human melanoma cell A375. Journal of Chinese Integrative Medicine, 2010, 8(9): 853-862.
[15] Bing Hu, Hong-mei An, Ke-ping Shen, Qin Du. Effects of Tenglong Buzhong Decoction on proliferation and apoptosis of human colon carcinoma cell line LS174T. Journal of Chinese Integrative Medicine, 2010, 8(6): 575-580.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[an error occurred while processing this directive]