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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (1): 30-34.doi: 10.3736/jcim20060109

• Original Experimental Research • Previous Articles     Next Articles

Effects of active components extracted from Qixue Bingzhi Recipe on proliferation of vascular smooth muscle cells and expressions of platelet-derived growth factor and its receptor genes

Bo Ji1, Ping Geng2, Jian-gang Liu1, Da-zhuo Shi1, Yong-yan Wang3   

  1. 1.Department of Cardiology, Xiyuan Hospital, China Academy of Traditional Chinese Medical Science, Beijing 100091, China
    2.Department of Internal Medicine, Mudanjang Hospital of Traditional Chinese Medicine, Mudanjiang, Heilongjiang Province 157000, China
    3.China Academy of Traditional Chinese Medical Science, Beijing 100071, China
  • Online:2006-01-20 Published:2006-01-15

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Objective

To observe the effects of paeoniflorin and total flavones extracted from Qixue Bingzhi Recipe on proliferation of vascular smooth muscle cells ( VSMCs ) cultured in endothelial cell conditioned medium ( EC-CM ) induced by oxidized low-density lipoprotein (ox-LDL ) and the expressions of plateletderived growth factor and its receptor genes. 

Methods

The VSMCs were cultured in normal culture medium, EC-CM, simvastatin-medicated EC-CM, paeoniflorin and total flavones (low, medium and high dose) -medicated EC-CM respectively . The growth activity of VSMCs was assessed by thiazolyl blue tetrazolium bromide (MTT) assay. The expressions of platelet-derived growth factor ( PDGF) -BB and platelet-derived growth factor receptor-α (PDGFR-α) mRNAs were detected by reverse transcription polymerase chain reaction (RTPCR). 

Results

The growth activity of VSMCs cultured in the EC-CM induced by ox-LDL was significantly higher than that in the normal culture medium ( P < 0 .01 ) , and the expressions of PDGF-BB and PDGFR-α mRNAs were obviously increased as compared with those in the normal culture medium . The growth activity of VSMCs cultured in each paeoniflorin and total flavones-medicated EC-CM was significantly lower than that in the EC-CM ( P < 0 .01) , and the expressions of PDGF-BB and PDGFR-α mRNAs were obviously decreased as compared with those in the EC-CM. 

Conclusion

The paeoniflorin and total flavones extracted from Qixue Bingzhi Recipe may be beneficial to the prevention and treatment of arteriosclerosis, and this efficacy may be correlated with down-regulating the expressions of PDGF-BB and PDGFR-α mRNAs which are related to the proliferation of VSMCs cultured in EC-CM induced by ox-LDL.

Key words: Arteriosclerosis, Endothelial cells, Myocytes, Smooth muscle, Lipoprotein, Platelet-derived growth factor, Qixue Bingzhi Recipe, Xuefu Zhuyu Decoction

CLC Number: 

  • R286.2

"

Culture medium n Optical density value
Normal culture medium 5 1.63±0.08
EC-CM 5 3.08±0.51**
Simvastatin-medicated EC-CM (1 μmol/ml) 5 2.20±0.16△△
Paeoniflorin and total flavones-medicated EC-CM (10 μg/ml) 5 2.77±0.61
Paeoniflorin and total flavones-medicated EC-CM (1 μg/ml) 5 1.65±0.06△△
Paeoniflorin and total flavones-medicated EC-CM (0.1 μg/ml) 5 3.15±0.58

Figure 1

Agarose gel electrophoresis results of VSMCs RNA1: Cultured in normal culture medium; 2: Cultured in EC-CM; 3: Cultured in simvastatin-medicated EC-CM (1 μmol/ml); 4: Cultured in paeoniflorin and total flavones-medicated EC-CM (10 μg/ml); 5: Cultured in paeoniflorin and total flavones-medicated EC-CM (1 μg/ml); 6: Cultured in paeoniflorin and total flavones-medicated EC-CM (0.1 μg/ml)."

Figure 2

RT-PCR results of expression of PDGF-BB mRNA1: Cultured in normal culture medium; 2: Cultured in EC-CM; 3: Cultured in simvastatin-medicated EC-CM (1 μmol/ml); 4: Cultured in paeoniflorin and total flavones-medicated EC-CM (10 μg/ml); 5: Cultured in paeoniflorin and total flavones-medicated EC-CM (1 μg/ml); 6: Cultured in paeoniflorin and total flavones-medicated EC-CM (0.1 μg/ml)."

Figure 3

RT-PCR results of expression of PDGFR-α mRNA1: Cultured in normal culture medium; 2: Cultured in EC-CM; 3: Cultured in simvastatin-medicated EC-CM (1 μmol/ml); 4: Cultured in paeoniflorin and total flavones-medicated EC-CM (10 μg/ml); 5: Cultured in paeoniflorin and total flavones-medicated EC-CM (1 μg/ml); 6: Cultured in paeoniflorin and total flavones-medicated EC-CM (0.1 μg/ml)."

[1] 张红霞, 刘剑刚, 史大卓 . 气血并治方对颈动脉粥样硬化患者超敏C反应蛋白等指标的影响[J]. 中医杂志, 2004,45(12):907-910
[2] 嵇波, 宋晓东, 刘剑刚 , 等. 氧化低密度脂蛋白诱导血管内皮细胞损伤的条件培养基对血管平滑肌细胞增殖的作用[J]. 中国分子心脏病学杂志, 2004,4(5):279-283
doi: 10.3969/j.issn.1671-6272.2004.05.006
[3] Lusis AJ . Atherosclerosis[J]. Nature, 2000,407(6801):233-241
[4] Rivard A, Andres V . Vascular smooth muscle cell proliferation in the pathogenesis of atherosclerotic cardiovascular diseases[J]. Histol Histopathol, 2000,15(2):557-571
[5] 陈在嘉, 高润霖 .冠心病 ( 第1版)[M]. 北京: 人民卫生出版社, 2002,( 第1版):36-40
[6] Ross R, Glomset J, Harker L . Response to injury and atherogenesis[J]. Am J Pathol, 1977,86(3):675-684
pmid: 842616
[7] Grotendorst GR, Chang T, Seppa HE , et al. Platelet-derived growth factor is a chemoattractant for vascular smooth muscle cells[J]. J Cell Physiol, 1982,113(2):261-266
doi: 10.1002/jcp.1041130213 pmid: 6184376
[8] Heldin CH, Westermark B . Mechanism of action and in vivo role of platelet-derived growth factor[J]. Physiol Rev, 1999,79(4):1283-1316
doi: 10.1152/physrev.1999.79.4.1283 pmid: 10508235
[9] 嵇波, 刘剑刚, 史大卓 , 等. 气血并治方有效组分不同配伍对血管内皮细胞损伤条件培养基诱导血管平滑肌细胞增殖的影响[J]. 北京中医药大学学报, 2005,28(1):30-33
doi: 10.3321/j.issn:1006-2157.2005.01.010
[10] Innis MA, Gelfand DH, Sninsky JJ. PCR protocol: A guide to methods and applications(1st ed)[M]. San Diego: Academic Press, 1990,( 1st ed):3-12
[11] Grotendorst G, Seppa HE, Kleinman HK , et al. Attachment of smooth muscle cells to collagen and their migration toward platelet-derived growth factor[J]. Proc Natl Acad Sci U S A, 1981,78(6):3669-3672
Grotendorst G, Seppa HE, Kleinman HK , et al. cccc[J]. Proc Natl Acad Sci U S A, 1981,78(6):3669-3672
[12] Ross R . The pathogenesis of atherosclerosis: a perspective for the 1990s[J]. Nature, 1993,362(6423):801-809
doi: 10.1038/362801a0 pmid: 8479518
[13] Reape TJ, Kanczler JM, Ward JP , et al. IGF-1 increases bFGF-induced mitogenesis and upregulates FGFR-1 in rabbit vascular smooth muscle cells[J]. Am J Physiol, 1996,270(4 Pt 2):H1141-H1148
[14] Bourcier T, Dockter M, Hassid A . Synergistic interaction of interleukin-1beta and growth factors in primary cultures of rat aortic smooth muscle cells[J]. J Cell Physiol, 1995,164(3):644-657
[15] Seifert RA, Hart CE, Phillips PE , et al. Two different subunits associate to create isoform-specific platelet-derived growth factor receptor[J]. J Biol Chem, 1989,264(15):8771-8778
[16] 覃军, 何作云, 万瑛 , 等. 辛伐他汀含药血清对兔血管平滑肌细胞增殖及原癌基因表达的作用和意义[J]. 中国血液流变学杂志, 2004,3(14):295-298
doi: 10.3969/j.issn.1009-881X.2004.03.005
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