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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (1): 30-34.doi: 10.3736/jcim20060109

• Original Experimental Research • Previous Articles     Next Articles

Effects of active components extracted from Qixue Bingzhi Recipe on proliferation of vascular smooth muscle cells and expressions of platelet-derived growth factor and its receptor genes

Bo Ji1, Ping Geng2, Jian-gang Liu1, Da-zhuo Shi1, Yong-yan Wang3   

  1. 1.Department of Cardiology, Xiyuan Hospital, China Academy of Traditional Chinese Medical Science, Beijing 100091, China
    2.Department of Internal Medicine, Mudanjang Hospital of Traditional Chinese Medicine, Mudanjiang, Heilongjiang Province 157000, China
    3.China Academy of Traditional Chinese Medical Science, Beijing 100071, China
  • Online:2006-01-20 Published:2006-01-15

Objective

To observe the effects of paeoniflorin and total flavones extracted from Qixue Bingzhi Recipe on proliferation of vascular smooth muscle cells ( VSMCs ) cultured in endothelial cell conditioned medium ( EC-CM ) induced by oxidized low-density lipoprotein (ox-LDL ) and the expressions of plateletderived growth factor and its receptor genes. 

Methods

The VSMCs were cultured in normal culture medium, EC-CM, simvastatin-medicated EC-CM, paeoniflorin and total flavones (low, medium and high dose) -medicated EC-CM respectively . The growth activity of VSMCs was assessed by thiazolyl blue tetrazolium bromide (MTT) assay. The expressions of platelet-derived growth factor ( PDGF) -BB and platelet-derived growth factor receptor-α (PDGFR-α) mRNAs were detected by reverse transcription polymerase chain reaction (RTPCR). 

Results

The growth activity of VSMCs cultured in the EC-CM induced by ox-LDL was significantly higher than that in the normal culture medium ( P < 0 .01 ) , and the expressions of PDGF-BB and PDGFR-α mRNAs were obviously increased as compared with those in the normal culture medium . The growth activity of VSMCs cultured in each paeoniflorin and total flavones-medicated EC-CM was significantly lower than that in the EC-CM ( P < 0 .01) , and the expressions of PDGF-BB and PDGFR-α mRNAs were obviously decreased as compared with those in the EC-CM. 

Conclusion

The paeoniflorin and total flavones extracted from Qixue Bingzhi Recipe may be beneficial to the prevention and treatment of arteriosclerosis, and this efficacy may be correlated with down-regulating the expressions of PDGF-BB and PDGFR-α mRNAs which are related to the proliferation of VSMCs cultured in EC-CM induced by ox-LDL.

Key words: Arteriosclerosis, Endothelial cells, Myocytes, Smooth muscle, Lipoprotein, Platelet-derived growth factor, Qixue Bingzhi Recipe, Xuefu Zhuyu Decoction

CLC Number: 

  • R286.2

"

Culture medium n Optical density value
Normal culture medium 5 1.63±0.08
EC-CM 5 3.08±0.51**
Simvastatin-medicated EC-CM (1 μmol/ml) 5 2.20±0.16△△
Paeoniflorin and total flavones-medicated EC-CM (10 μg/ml) 5 2.77±0.61
Paeoniflorin and total flavones-medicated EC-CM (1 μg/ml) 5 1.65±0.06△△
Paeoniflorin and total flavones-medicated EC-CM (0.1 μg/ml) 5 3.15±0.58

Figure 1

Agarose gel electrophoresis results of VSMCs RNA1: Cultured in normal culture medium; 2: Cultured in EC-CM; 3: Cultured in simvastatin-medicated EC-CM (1 μmol/ml); 4: Cultured in paeoniflorin and total flavones-medicated EC-CM (10 μg/ml); 5: Cultured in paeoniflorin and total flavones-medicated EC-CM (1 μg/ml); 6: Cultured in paeoniflorin and total flavones-medicated EC-CM (0.1 μg/ml)."

Figure 2

RT-PCR results of expression of PDGF-BB mRNA1: Cultured in normal culture medium; 2: Cultured in EC-CM; 3: Cultured in simvastatin-medicated EC-CM (1 μmol/ml); 4: Cultured in paeoniflorin and total flavones-medicated EC-CM (10 μg/ml); 5: Cultured in paeoniflorin and total flavones-medicated EC-CM (1 μg/ml); 6: Cultured in paeoniflorin and total flavones-medicated EC-CM (0.1 μg/ml)."

Figure 3

RT-PCR results of expression of PDGFR-α mRNA1: Cultured in normal culture medium; 2: Cultured in EC-CM; 3: Cultured in simvastatin-medicated EC-CM (1 μmol/ml); 4: Cultured in paeoniflorin and total flavones-medicated EC-CM (10 μg/ml); 5: Cultured in paeoniflorin and total flavones-medicated EC-CM (1 μg/ml); 6: Cultured in paeoniflorin and total flavones-medicated EC-CM (0.1 μg/ml)."

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