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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (2): 185-189.doi: 10.3736/jcim20080216

• Original Experimental Research • Previous Articles     Next Articles

Effects of Yisui Jiedu Recipe on JAK2-STAT5 signal transduction pathway in bone marrow hematopoietic cells from patients with myelodysplastic syndrome-refractory anemia

Sheng-li Tian1(), Yong-ming Zhou2, Tao Huang2, Zhi-zhong Xue2, Wei He2   

  1. 1. Department of Hematology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Department of Hematology, Yueyang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2007-04-11 Online:2008-02-20 Published:2008-02-15
  • Contact: TIAN Sheng-li E-mail:victorytian@126.com

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Objective: To investigate the effect of Yisui Jiedu Recipe (YSJDR), a compound traditional Chinese herbal medicine, on cytokines and their corresponding just another kinase 2-signal transducers and activators of transcription 5 (JAK2-STAT5) signal transduction pathway in bone marrow hematopoietic cells from patients with myelodysplastic syndrome-refractory anemia (MDS-RA).

Methods: Fluorogenic quantitative polymerase chain reaction (FQ-PCR) method was established to detect the levels of JAK2, STAT5 and Bcl-xL mRNA expressions, and JAK2-STAT5 signal transduction pathway was activated by granulocyte-macrophage-colony stimulating factor (GM-CSF) in cultured bone marrow hematopoietic cells from 10 patients with MDS-RA. The levels of interleukin-2 (IL-2), interleukin-3 (IL-3), γ-interferon (γ-INF) and tumor necrosis factor-α (TNF-α) in the cultural supernatant of untreated control, AG490-treated and YSJDF-treated cells were measured by enzyme-linked immunosorbent assay.

Results: The levels of IL-2 and TNF-α in YSJDR-treated group were significantly lower than those in untreated control group and AG490-treated group (P<0.01, P<0.05), and IL-3 level in YSJDP-treated group was remarkably higher than that in the other two groups (P<0.01). There were no significant differences in the levels of IL-2 and IL-3 between AG490-treated group and untreated control group (P>0.05), while the TNF-α level in AG490-treated group was decreased obviously as compared with the untreated control group (P<0.01). There was no significant difference in γ-INF level between YSJDR-treated group and AG490-treated group (P>0.05), while TNF-α level in the two groups were significantly lower than that in the untreated control group (P<0.01). The expressions of JAK2, STAT5 and Bcl-xL mRNAs were significantly down-regulated in the YSJDR-treated and the AG490-treated groups as compared with those in the untreated control group (P<0.05, P<0.01), while there were no differences in the expressions of JAK2, STAT5 and Bcl-xL mRNAs between YSJDR-treated group and AG490-treated group.

Conclusion: YSJDR can modulate cytokine level in bone marrow hematopoietic cells of MDS-RA, suppress JAK2-STAT5 signal transduction, and inhibit the Bcl-xL mRNA expression.

Key words: myelodysplastic syndromes, composite (traditional compound drug), cytokines, signal transduction

CLC Number: 

  • R556.5

Table 1

Levels of some cytokine expressions in different groups of cultured cells ($\bar{x}$±s, ng/L)"

Group n Level of cytokine expression
IL-2 IL-3 γ-INF TNF-α
Untreated control 10 24.64±18.50 42.19±44.42 62.71±38.92 66.65±25.58
AG490-treated 10 23.09±13.17 21.13±33.80 4.67±1.80** 17.12±14.65**
YSJDR-treated 10 8.12±6.55**△ 106.13±40.31**△△ 6.04±13.63** 10.78±19.40**△

Table 2

Signal gene mRNA expressions in three groups of cultured cells ($\bar{x}$±s)"

Group n JAK2 STAT5 Bcl-xL
Untreated control 10 5 693.31±3 372.60 1 697.70±598.03 1 198.46±539.96
AG490-treated 10 680.39±557.62** 58.37±40.26** 335.91±270.21**
YSJDP-treated 10 1 895.36±2 285.14** 378.91±467.82** 368.75±671.41*
[1] Coffer PJ , Koenderman L,de Groot RP. The role of STATs in myeloid differentiation and leukemia. Oncogene. 2000; 19(21):2511-2522.
doi: 10.1038/sj.onc.1203479 pmid: 10851050
[2] Spiekermann K, Biethahn S, Wilde S , et al . Constitutive activation of STAT transcription factors in acute myelogenous leukemia. Eur J Haematol. 2001; 67(2):63-71.
doi: 10.1034/j.1600-0609.2001.t01-1-00385.x pmid: 011722592
[3] Zhang ZN. Diagnostic and effect evaluation standards of hematology. 2nd ed. Beijing: Science Press. 1998: 263-295. Chinese.
张之南 . 血液病诊断及疗效标准.第2版.北京: 科学出版社. 1998: 263-295.
[4] Steelman LS, Pohnert SC, Shelton JG , et al . JAK/ STAT, Raf/MEK/ERK, PI3K/Akt and BCR-ABL in cell cycle progression and leukemogenesis. Leukemia. 2004; 18(2):189-218.
doi: 10.1038/sj.leu.2403241
[5] Kochendoerfer SK, Krishnan N, Buckley DJ , et al . Prolactin regulation of Bcl-2 family members: increased expression of bcl-xL but not mcl-1 or bad in Nb2-T cells. J Endocrinol. 2003; 178(2):265-273.
[6] Sultana TA, Harada H, Ito K , et al . Expression and functional analysis of granulocyte colony-stimulating factor receptors on CD34 ++ cells in patients with myel- odysplastic syndrome (MDS) and MDS-acute myeloid leukaemia. Br J Haematol. 2003; 121(1) : 63-75.
[7] Ihle JN . Signaling by the cytokine receptor superfamily in normal and transformed hematopoietic cells. Adv Cancer Res. 1996; 68(1):23-65.
doi: 10.1016/S0065-230X(08)60351-6
[8] Tian SL. Experience of treatment of myelodysplastic syndrome by Zhou Yongming. Zhong Yi Za Zhi. 2005; 46(1):14-15. Chinese.
doi: 10.3321/j.issn:1001-1668.2005.01.009
田胜利 . 周永明治疗骨髓增生异常综合征的经验.中医杂志. 2005; 46(1):14-15.
doi: 10.3321/j.issn:1001-1668.2005.01.009
[9] Tian SL, Zhou YM, Hu MH , et al . Treatment of myelodysplastic syndrome by improved prescription of Shengxue Heji: A clinical observation of 60 cases. Xin Zhong Yi. 2005; 37(3) : 22-24. Chinese.
doi: 10.3969/j.issn.0256-7415.2005.03.010
田胜利, 周永明, 胡明辉 , 等. 改良生血合剂治疗骨髓增生异常综合症60例疗效观察.新中医. 2005; 37(3) : 22-24.
doi: 10.3969/j.issn.0256-7415.2005.03.010
[10] Tian SL, Huang T, Zhou YM , et al. Effects of "Yisui Integrative Decoction" on IL-3 and IFN-y of MDS-RA marrow system. Shanghai Zhong Yi Yao Za Zhi. 2005; 39(2):26-28. Chinese with abstract in English.
doi: 10.3969/j.issn.1007-1334.2005.02.012
田胜利, 黄韬, 周永明 , 等. 益髓解毒方对MDS-RA骨髓培养体系IL-3、IFN-γ的影响.上海中医药杂志. 2005; 39(2):26-28.
doi: 10.3969/j.issn.1007-1334.2005.02.012
[11] Wang KM, Wang B , He DL. Current status of research on JAK/STAT pathway blocker AG490. Xian Dai Mi Niao Wai Ke Za Zhi. 2003; 8(1):57-59. Chinese.
doi: 10.3969/j.issn.1009-8291.2003.01.047
王克明, 王彬, 贺大林 . JAK/STAT通路阻断剂 AG490的研究现状.现代泌尿外科杂志. 2003; 8(1):57-59.
doi: 10.3969/j.issn.1009-8291.2003.01.047
[12] Tian SL, Zhou YM, Ge L , et al. The effect of AG490 on JAK2/STAT5 signal transduction pathway in bone marrow hematopoietic cells of MDS-RA. Jian Yan Yi Xue. 2006; 21(3):278-280. Chinese with abstract in English.
doi: 10.3969/j.issn.1673-8640.2006.03.031
田胜利, 周永明, 葛垒 , 等. MDS~RA骨髓造血细胞 JAK2/STAT5通路活化及AG490干预研究. 检验医学. 2006; 21(3):278-280.
doi: 10.3969/j.issn.1673-8640.2006.03.031
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