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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (10): 974-978.doi: 10.3736/jcim20101010

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Bushen Shugan Recipe in regulating the hypothalamus-pituitary-ovarian axis in a rat model of stress-induced anorexia

WX Hu,CQ Yu,DX Zhai,J Han,DY Zhang   

  1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2010-05-16 Accepted:2010-08-31 Online:2010-10-20 Published:2010-10-15

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Objective

To investigate the effects of Bushen Shugan Recipe (BSSGR), a compound traditional Chinese herbal medicine, in regulating the hypothalamus-pituitary-ovarian axis (HPOA) in a rat model of stress-induced anorexia.
Methods

Anorexia was induced in rats by the methods of separation, diet restriction and constraint. Rats were divided into 4 groups randomly: control group, untreated group, sham-operated group and BSSGR group. After the experiments, body weights and oestrous cycles of the 4 groups were compared. The levels of serum estradiol (E2), hypophysis luteotrophic hormone (LH), hypophysis follicle stimulating hormone (FSH) and hypothalamus β-endorphin (β-EP) were detected by radioimmunoassay. The level of serum corticosterone (CORT) was detected by enzyme-linked immunosorbent assay.

Results

Body weight of BSSGR group was significantly increased in comparison with sham-operated group(P<0.01); the oestrous cycle disordering rate was higher than those of the untreated group and sham-operated group; hypophysis LH and serum E2 were obviously increased in comparison with untreated group (P<0.05); hypothalamus β-EP was obviously decreased in comparison with sham-operated group (P<0.05); serum CORT was obviously decreased in comparison with untreated group (P<0.05）, and significantly decreased in comparison with sham-operated group (P<0.01).
Conclusion

BSSGR increased hypophysis LH and serum E2, and decreased serum CORT and hypothalamus β-EP in rats with stress-induced anorexia.

Key words: Compounds(TCD), Anorexia nervosa, Stress models, Hypothalamus-pituitary-ovarian axis

Table 1

Body weights of rats in different groups"

Table 2

Levels of hypophysis FSH and LH and serum E2 of rats in different groups"

Table 3

Levels of hypothalamus β-EP and serum CORT of rats in different groups"

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