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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 647-650.doi: 10.3736/jcim20070609

• Original Experimental Research • Previous Articles     Next Articles

Effects of arsenic trioxide on expressions of vascular endothelial growth factor and P-glycoprotein in multidrug resistant leukemia cell line K562/A02

Hong Liang1, Yu Zhang1, Jian-dong Zhang1, Jian Gu2, Li Ma2, Xiao-ling Wang2, Wei-gan Shen1   

  1. 1. Medical College,Yangzhou University,Yangzhou,Jiangsu Province 225001,China
    2. Yangzhou Institute of Hematology,Yangzhou,Jiangsu Province 225001,China
  • Online:2007-11-20 Published:2007-11-15

Objective: To investigate the effects of arsenic trioxide (ATO) on the expressions of vascular endothelial growth factor (VEGF) and P-glycoprotein (P-gp) in K562/A02 cells and to explore the correlation between VEGF and P-gp.Methods: The inhibition rate of K562/A02 cell proliferation was detected by using methyl thiazolyl tetrazolium assay (MTT); the level of VEGF was detected by enzyme-linked immunosorbent assay (ELISA) and the expression rate of P-gp was determined by flow cytometry (FCM).Results: 0.05 μmol/L ATO had no influences on the cell proliferation and the expression of VEGF in K562/A02 cells; 0.4 and 3.2 μmol/L ATO could significantly inhibit the K562/A02 cell proliferation and down-regulate the expression of VEGF in K562/A02 cells (P<0.05). The expression of P-gp did not changed after being exposed to 0.05 and 0.4 μmol/L ATO for 24, 48 and 72 hours (P>0.05). 3.2 μmol/L ATO could remarkably reduce the expression of P-gp in K562/A02 cells after 48- and 72-hour incubation with ATO (P<0.05).Conclusions: The down-regulation of P-gp and VEGF after being exposed to ATO probably contributes to the reversion of multidrug resistance in K562/A02 cells. The level of VEGF may be related to the expression rate of P-gp in K562/A02 cells.

Key words: vascular endothelium, endothelial growth factors, arsenic compounds, leukemia

CLC Number: 

  • R733.7

Figure 1

Inhibition rates of K562/A02 and K562/S cells treated with different concentrations of ATO"

Table 1

Effects of ATO on expression of VEGF in K562/A02 cells (ヌ±S, ng/L)"

Group n The levels of VEGF in different action time
24 h 48 h 72 h
Blank control 6 772.93±22.00 1 137.17±77.85 2 957.23±92.15
0.05 μmol/L ATO-treated 6 785.13±31.85 1 194.63±90.68 3 005.43±111.47
0.4 μmol/L ATO-treated 6 440.13±29.70* 548.07±24.00* 1 741.97±122.45*
3.2 μmol/L ATO-treated 6 199.00±23.79* 410.57±55.99* 1 098.23±73.78*

Table 2

Effects of ATO on expression of P-gp in K562/A02 cells (ヌ±S, %)"

Group n The levels of P-gp in different action time
24 h 48 h 72 h
Blank control 6 61.78±1.33 68.20±1.38 70.31±5.58
0.05 μmol/L ATO-treated 6 61.41±6.01 66.10±2.03 71.96±1.14
0.4 μmol/L ATO-treated 6 62.44±1.68 63.12±2.03 71.02±2.68
3.2 μmol/L ATO-treated 6 63.96±0.91 62.68±1.71* 42.90±3.43*
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