三氧化二砷对耐药白血病细胞血管内皮生长因子和P-糖蛋白表达的影响

doi:10.3736/jcim20070609

Abstract

Objective: To investigate the effects of arsenic trioxide (ATO) on the expressions of vascular endothelial growth factor (VEGF) and P-glycoprotein (P-gp) in K562/A02 cells and to explore the correlation between VEGF and P-gp.Methods: The inhibition rate of K562/A02 cell proliferation was detected by using methyl thiazolyl tetrazolium assay (MTT); the level of VEGF was detected by enzyme-linked immunosorbent assay (ELISA) and the expression rate of P-gp was determined by flow cytometry (FCM).Results: 0.05 μmol/L ATO had no influences on the cell proliferation and the expression of VEGF in K562/A02 cells; 0.4 and 3.2 μmol/L ATO could significantly inhibit the K562/A02 cell proliferation and down-regulate the expression of VEGF in K562/A02 cells (P<0.05). The expression of P-gp did not changed after being exposed to 0.05 and 0.4 μmol/L ATO for 24, 48 and 72 hours (P>0.05). 3.2 μmol/L ATO could remarkably reduce the expression of P-gp in K562/A02 cells after 48- and 72-hour incubation with ATO (P<0.05).Conclusions: The down-regulation of P-gp and VEGF after being exposed to ATO probably contributes to the reversion of multidrug resistance in K562/A02 cells. The level of VEGF may be related to the expression rate of P-gp in K562/A02 cells.

Key words: vascular endothelium, endothelial growth factors, arsenic compounds, leukemia

CLC Number:

R733.7

Hong Liang, Yu Zhang, Jian-dong Zhang, Jian Gu, Li Ma, Xiao-ling Wang, Wei-gan Shen. Effects of arsenic trioxide on expressions of vascular endothelial growth factor and P-glycoprotein in multidrug resistant leukemia cell line K562/A02. Journal of Chinese Integrative Medicine, 2007, 5(6): 647-650.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20070609

http://www.jcimjournal.com/EN/Y2007/V5/I6/647

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References 11

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Group	n	The levels of VEGF in different action time
Group	n	24 h	48 h	72 h
Blank control	6	772.93±22.00	1 137.17±77.85	2 957.23±92.15
0.05 μmol/L ATO-treated	6	785.13±31.85	1 194.63±90.68	3 005.43±111.47
0.4 μmol/L ATO-treated	6	440.13±29.70*	548.07±24.00*	1 741.97±122.45*
3.2 μmol/L ATO-treated	6	199.00±23.79*	410.57±55.99*	1 098.23±73.78*

Group	n	The levels of P-gp in different action time
Group	n	24 h	48 h	72 h
Blank control	6	61.78±1.33	68.20±1.38	70.31±5.58
0.05 μmol/L ATO-treated	6	61.41±6.01	66.10±2.03	71.96±1.14
0.4 μmol/L ATO-treated	6	62.44±1.68	63.12±2.03	71.02±2.68
3.2 μmol/L ATO-treated	6	63.96±0.91	62.68±1.71*	42.90±3.43*

Effects of arsenic trioxide on expressions of vascular endothelial growth factor and P-glycoprotein in multidrug resistant leukemia cell line K562/A02

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