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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (3): 210-212.doi: 10.3736/jcim20040318

• Original Experimental Research • Previous Articles     Next Articles

Effect of Compound Rhubarb Mixture on contraction of isolated small intestinal smooth muscle of rabbit

Hong-quan Zhang, Zheng-xin Xu, Yu-qing Wu, Cheng-hua Zhou   

  1. Medical and Pharmacological Institute, Yangzhou University, Yangzhou, Jiangsu Province 225001, China
  • Online:2004-06-20 Published:2018-10-25

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Objective: To investigate the effect of Compound Rhubarb Mixture on contraction of isolated small intestinal smooth muscle of rabbit. Methods: Serum containing Compound Rhubarb Mixture was prepared. The contraction curves of isolated small intestinal smooth muscle before and after administration of the serum were recorded by Medlab biological information collecting system. Results: The serum containing Compound Rhubarb Mixture amplified the contraction amplitude of isolated small intestinal smooth muscle of rabbit. The rate of change of contraction amplitude was elevated significantly after administration, while the frequency of contraction did not change obviously. Conclusion: The serum containing Compound Rhubarb Mixture can enhance the contractive function of isolated small intestinal smooth muscle of rabbit.

Key words: Compound Rhubarb Mixture, serum, isolated small intestine, physiological activity

CLC Number: 

  • R363

Figure 1

Contraction curves of isolated small intestinal smooth muscle before and after administration of serum in 4 groups A： Normal serum; B： Serum containing Compound Rhubarb Mixture (1∶5 dilution); C： Serum containing Compound Rhubarb Mixture (1∶2 dilution); D： Serum containing Compound Rhubarb Mixture (no dilution)"

Figure 2

Effect of serum containing Compound Rhubarb Mixture on contraction amplitude of isolated small intestinal smooth muscle(n=8, ヌ±S) □: Before administration; ■: After administration; A： Normal serum; B： Serum containing Compound Rhubarb Mixture (1∶5 dilution); C：Serum containing Compound Rhubarb Mixture (1∶2 dilution); D：Serum containing Compound Rhubarb Mixture (no dilution); **P<0.01, vs before administration in the same group;△△P<0.01, vs normal serum"

Tab 1

Effect of serum containing Compound Rhubarb Mixture on the rate of change of contraction amplitude of isolated small intestinal smooth muscle (ヌ±S)"

Group n The rate of change of
contraction amplitude (%)
Serum Volume (μl)
Normal serum 50 8 10.62±2.33
Serum containing Compound Rhubarb Mixture (1∶5 dilution) 50 8 70.24±9.65△△
Serum containing Compound Rhubarb Mixture (1∶2 dilution) 50 8 87.86±11.56△△
Serum containing Compound Rhubarb Mixture (no dilution) 50 8 138.38±17.91△△

Tab 2

Effect of serum containing Compound Rhubarb Mixture on frequency of contraction of isolated small intestinal smooth muscle (ヌ±S)"

Group n
 Frequency (contractions/min)
Serum Volume (μl) Before administration After administration
Normal serum 50 8 10.25±2.20 10.80±2.35
Serum containing Compound Rhubarb Mixture (1∶5 dilution) 50 8 11.12±2.40 10.95±2.15
Serum containing Compound Rhubarb Mixture (1∶2 dilution) 50 8 11.05±2.48 11.20±2.26
Serum containing Compound Rhubarb Mixture (no dilution) 50 8 10.94±2.32 11.06±2.30
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