SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 514-516.doi: 10.3736/jcim20070507

Special Issue: Traditional Chinese Medicine

• Original Clinical Research • Previous Articles     Next Articles

Serum proteomics study of chronic gastritis with dampness syndrome in traditional Chinese medicine

Yi-qin Wang1, Fu-feng Li1,Wen-jing Wang2, Li-yu Zhao1, Li Guo1, Hui-fang Wang1   

  1. 1. Laboratory of Syndromes of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China
    2. Department of Molecular Biology for Public Health,Shanghai Municipal Center for Disease Control and Prevention ，Shang hai 200336,China
  • Online:2007-09-20 Published:2007-09-15

RichHTML

1

PDF (PC)

185

Like

Objective: To explore microcosmic information in chronic gastritis dampness syndrome by using serum proteomics of patients with chronic gastritis dampness syndrome.Methods: Serum proteomics of 18 dampness syndrome cases, 17 non-dampness syndrome cases in chronic gastritis patients and 8 normal controls were analyzed by surface enhanced laser desorption/ionization-time of flight (SELDI-TOF) protein-chip. Results: There was a high expression trend in three ratios of charge (of an electron) to mass (M/S) of 3.2 kD, 6.4 kD and 8.1 kD of protein expression spectrum from patients with chronic gastritis dampness syndrome, and the wave peak value was over 10 units. There was a low expression trend in above-mentioned sites of protein expression spectrum from patients with chronic gastritis non-dampness syndrome and normal group. The wave peak value of patients with chronic gastritis non-dampness syndrome was about or below 10 units, and the wave peak value in normal group was below 5 units. Conclusion: There exists a certain significance to explore biologic theory basis of chronic gastritis dampness syndrome by analyzing the serum proteomics.

Key words: chronic gastritis, dampness syndrome, serum, proteomics

CLC Number: 

  • R573.3

Table 1

Screening test"

Screening test Patients with chronic gastritis Normal people
Positive（+） A B
Negative（–） C D

Figure 1

Protein expression spectrumA: Patients with chronic gastritis dampness syndrome; B: Patients with chronic gastritis non-dampness syndrome; C: Normal people."

[1] 方福德 . 人类基因组图谱研究能给我们带来什么[J]. 医学与哲学, 2000,21(11):16-20
Fang FD . What does the human genome research bring us[J]. Yi Xue Yu Zhe Xue, 2000,21(11):16-20
[2] 中华医学会消化病学分会. 全国慢性胃炎研讨会共识意见[J]. 中华消化杂志, 2000,20(3):199-201
doi: 10.3760/j.issn:0254-1432.2000.03.015
Digestive Disease Branch of Chinese Medical Association. Consensus documents of chronic gastritis symposium[J]. Zhonghua Xiao Hua Za Zhi, 2000,20(3):199-201
doi: 10.3760/j.issn:0254-1432.2000.03.015
[3] 陈贵延, 杨思澍 . 实用中西医结合诊断治疗学[M]. 北京: 中国医药科技出版,1991,(): 432-433
Chen GY, Yang SS. Practical diagnostics and therapeutics of integrated traditional Chinese and Western medicine[M]. Beijing: Chinese Press of Medical Science and Technology,1991,(): 432-433
[4] 刘为民 . 中医证候学研究与证候蛋白质组学[J]. 中医药学报, 2003,31(3):1
Liu WM . Study of traditional Chinese medicine syndrome and proteomics of syndrome[J]. Zhong Yi Yao Xue Bao, 2003,31(3):1
[5] Paweletz CP, Trock B, Pennanen M , et al. Proteomic patterns of nipple aspirate fluids obtained by SELDI-TOF: Potential for new biomarkers to aid in the diagnosis of breast cancer[J]. Dis Markers, 2001,17(4):301-307
[6] Chernyak A, Karavanov A, Ogawa Y , et al. Conjugating oligosaccharides to proteins by squaric acid diester chemistry: rapid monitoring of the progress of conjugation, and recovery of the unused ligand[J]. Carbohydrate Res, 2001,330(4):479-486
[7] 刘晓燕, 吕志平, 张绪富 . 蛋白质组及其在现代中医研究中的应用[J]. 中国中西医结合杂志, 2003,23(2):84-87
Liu XY, Lu ZP, Zhang XF . Concept of proteome and application of proteome in study of modern traditional Chinese medicine[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2003,23(2):84-87
[1] Lee Suan Chua, Siti Najihah Zukefl i. A comprehensive review of edible bird nests and swiftlet farming. Journal of Integrative Medicine, 2016, 14(6): 415-428.
[2] Fu-feng Li, Jie Zhao, Peng Qian, Yi-qin Wang, Jing-jing Fu, Zhu-mei Sun, Hai-xia Yan, Li Yang. Metabolite changes in the greasy tongue coating of patients with chronic gastritis. Journal of Chinese Integrative Medicine, 2012, 10(7): 757-765.
[3] Jing Zhou , Hong-yue Ma , Xin-sheng Fan , Wei Xiao , Tuan-jie Wang . Molecular docking of chlorogenic acid, 3,4-di-O-caffeoylquinic acid and 3,5-di-O-caffeoylquinic acid with human serum albumin. Journal of Chinese Integrative Medicine, 2012, 10(10): 1149-1154.
[4] Lin-na Yu, Jian Pei. Application of differential proteomics in mechanism research of acupuncture. Journal of Chinese Integrative Medicine, 2011, 9(8): 819-823.
[5] Guo-di Lu, Jing Zhou, Hong-yue Ma, De-kang Wu, Da-wei Qian, Yu-ping Tang, Ming Hong, Yong-qing Hua, Jing-ao Duan. Analysis on competitive interaction between arenobufagin and verapamil hydrochloride with serum albumin. Journal of Chinese Integrative Medicine, 2011, 9(7): 768-774.
[6] Mao Mei-jiao, Hu Jun-ping, Chen Fu-rong, Zhang Yi-yi, Liu Ping. Effects of Chinese herbal medicine Guanxinkang on lipid metabolism and serum C-reactive protein, amyloid A protein, and fibrinogen in apolipoprotein E-knockout mice with atherosclerosis. Journal of Chinese Integrative Medicine, 2011, 9(3): 306-312.
[7] Mei-qun Cao, Zheng-zhi Wu, Wei-kang Wu. Identification of salivary biomarkers in breast cancer patients with thick white or thick yellow tongue fur using isobaric tags for relative and absolute quantitative proteomics. Journal of Chinese Integrative Medicine, 2011, 9(3): 275-280.
[8] Shuo Zhang , Hong-cai Shang. Correlation between traditional Chinese medicine syndromes and omics: A literature review. Journal of Chinese Integrative Medicine, 2011, 9(12): 1286-1291.
[9] Jun Li, Bin Li, Wen-jiang Zhou, Fan-gui Zhao, Da-jin Li, Wen-jun Wang. Medicated rat serum containing Gengnianchun decoction reduces apoptosis of pheochromocytoma cells insulted by amyloid beta protein. Journal of Chinese Integrative Medicine, 2010, 8(5): 472-479.
[10] Mu-wei Gui, Pin-kang Wei, Ye Lu, Wei Guo, Zhi-feng Qin, Da-zhi Sun. Effects of Xiaotan Sanjie Decoction-containing serum on proliferation and apoptosis of human gastric cancer cells MKN-45. Journal of Chinese Integrative Medicine, 2010, 8(3): 250-255.
[11] Ding-zhu Shen , Qing Ta, Jin-xing Du, Sai-dan Ding, Gao-feng Chen, Yi-yang Ru, Ping Liu. Effects of Yiguanjian Decoction on liver cirrhosis formation： A differential proteomics study in rats. Journal of Chinese Integrative Medicine, 2010, 8(2): 158-167.
[12] Wen-jun Wang, Da-jin Li, Jun Li, Wen-jiang Zhou. An in vitro study on neuroprotective effects of serum containing Gengnianchun decoction and its main monomers against amyloid beta protein-induced cellular toxicity. Journal of Chinese Integrative Medicine, 2010, 8(1): 67-73.
[13] Li-bin Zhan , Xin-ping Niu, Hua Sui, Xiao-yang Gong. Protective effect of spleen-yin-nourishing recipe on amyloid β-peptide-induced damage of primarily cultured rat hippocampal neurons and its mechanism. Journal of Chinese Integrative Medicine, 2009, 7(3): 242-248.
[14] Guang-wei Zhong, Wei Li, Yan-hong Luo, Guo-lin Chen, Zhen-jia Yi, Jian-jun Hu, Ying Zhang, Yao-hui Yin, Le Zhang.
  • Herbs for calming liver and suppressing liver-yang in treatment of migraine with hyperactive liver-yang sydrome and its effects on lymphocyte protein expression: a randomized controlled trial
. Journal of Chinese Integrative Medicine, 2009, 7(1): 25-33.
[15] Zhi-jun Dai, Xi-jing Wang, Qian Xue, Zong-zheng Ji, Xiao-xu Liu, Hua-feng Kang, Hai-tao Guan, Xiao-bin Ma, Hong-tao Ren. Effects of Scutellaria Barbata drug-containing serum on apoptosis and mitochondrial transmembrane potential of hepatoma H22 cells. Journal of Chinese Integrative Medicine, 2008, 6(8): 821-826.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3