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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (5): 472-479.doi: 10.3736/jcim20100512

• Original Experimental Research • Previous Articles     Next Articles

Medicated rat serum containing Gengnianchun decoction reduces apoptosis of pheochromocytoma cells insulted by amyloid beta protein

 Jun Lia, Bin Lia, Wen-jiang Zhoub, Fan-gui Zhaoa, Da-jin Lia, Wen-jun Wanga   

  1. a Department of Integrated Traditional Chinese and Western Medicine,Gynecology and Obstetrics Hospital, Fudan University, Shanghai 200011, China
    b Department of Experimental Animal, Fudan University, Shanghai 200023, China
  • Received:2009-12-31 Accepted:2010-03-08 Online:2010-05-20 Published:2010-05-15
  • Contact: Wen-jun Wang E-mail:abby_lijun@163.com

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Objective

To investigate the effects of medicated rat serum containing Gengnianchun (GNC) decoction and its protection to pheochromocytoma cells (PC12 cells) from amyloid beta (Aβ)25-35-insulted apoptosis and to find the possible mechanism.
Methods

Medicated rat serum was prepared by administering ovariectomized Sprague-Dawley (SD) rats with GNC decoction. The effects of medicated rat serum on viability of PC12 cells were evaluated by cell counting kit-8 (CCK-8) assay. The PC12 cells were cultured with different doses of Aβ25-35 to induce a model of Alzheimer's disease in vitro. Then, the protectiv