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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (3): 281-284.doi: 10.3736/jcim20060312

• Original Experimental Research • Previous Articles     Next Articles

Establishment of a rat model of alcoholic liver fibrosis induced by complex factors

Lei Wang, Guang Ji, Pei-yong Zheng, Ai-hua Long   

  1. Laboratory of Fatty Liver Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Online:2006-05-31 Published:2006-05-15

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Objective

To establish a model of alcoholic liver fibrosis (ALF) in rats induced by complex factors.

Methods

Forty-seven healthy male rats were divided into three groups: normal control group (n=12), minor CCl4 group (n=12) and complex factors group (n=27). The rats in the complex factors group were fed a complex diet including alcohol, corn oil and pyrazole, and administered with intraperitoneal injection of minor CCl4 to induce ALF. During induction process, the histopathological changes of liver tissue and the values of liver-to-body weight ratio were both observed regularly. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyltransferase (γ-GT) in these three groups were all examined at the 12th week of the induction process.

Results

At the 12th week of the induction process, the model of ALF induced by complex factors was successfully established in rats, and the histopathological presentations showed alcoholic fatty liver, hepatitis and liver fibrosis in a sequence along with the induction process. The value of liver-to-body weight ratio and the serum levels of ALT, AST and γ-GT of rats in the complex factors group were all significantly different from those in the other two groups.

Conclusion

It is a steady and effective way to induce ALF in rats with complex diet and minor CCI4 injection.

Key words: Alcohol, Liver fibrosis, Animal models

CLC Number: 

  • 575.29

Table 1

Comparisons of liver-to-body weight ratio and serum levels of ALT, AST and γ-GT of rats among three groups（ヌ±S ）"

Group n Liver-to-body weight ratio (%) ALT (U/L) AST (U/L) γ-GT (U/L)
Normal control 12 2.6±0.2**△△ 22.5±14.0**△ 31.6±7.2**△ 84.8±19.0**
Minor CCl4 12 3.2±0.3** 45.0±16.6** 56.9±12.1** 100.7±21.5**
Complex factors 8 3.6±0.2 128.4±25.7△△ 267.2±51.0△△ 496.2±198.2△△

Figure 1

Histopathological changes of liver tissue in rats from different groupsA: Complex factors group at the 2nd week of induction process (HE staining, ×200); B: Complex factors group at the 4th week of induction process (HE staining, ×200); C: Complex factors group at the 6th week of induction process (HE staining, ×200); D: Complex factors group at the 8th week of induction process (HE staining, ×200); E: Complex factors group at the 10th week of induction process (HE staining, ×200); F: Complex factors group at the 12th week of induction process (HE staining, ×200); G: Complex factors group at the 12th week of induction process (Masson staining, ×200); H: Normal control group at the 12th week(HE staining, ×200); I: Normal control group at the 12th week (Masson staining, ×200); J: Minor CCl4 group at the 12th week of induction process (HE staining, ×200); K: Minor CCl4 group at the 12th week of induction process (Masson staining, ×200)."

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