Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (4): 392-396.doi: 10.3736/jcim20060415

• Original Experimental Research • Previous Articles     Next Articles

Radiosensitization of β-elemene on VX2 carcinoma transplanted on kidney in rabbits in vivo

Jun-jun She1,2, Zi-ming Wang1, Xiang-ming Che2, Cheng-en Pan2   

  1. 1. Department of Urology, Second Hospital, School of Medicine, Xi’an Jiaotong University,Xi’an, Shaanxi Province 710004, China;
    2. Department of General Surgery, First Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi Province 710061, China;
  • Online:2006-07-31 Published:2006-07-15

Objective

To investigate the radiosensitization of β-elemene on VX2 carcinoma transplanted on kidney in rabbits in vivo.

Methods

The rabbits were all transplanted with VX2 carcinoma on kidney. The appropriate dose of β-elemene infusion via renal artery for further study on radiosensitization was determined. Then fifty-five rabbits were divided into three groups: untreated group, radiation group and radiation plus β-elemene-treated group. After corresponding intervention for each group, the tumor volume was measured by ultrasonography and spiral computed tomography. The sensitization enhancement ratio (SER) of β-elemene was calculated. The pathological change of tumor tissue in kidey was observed by light microscopy and electron transmission microscopy. The apoptotic index was also examined by TdT-mediated dUTP-biotin nick end labeling method.

Results

The most significant radiosensitivity was observed in the radiation plus β-elemene-treated group with 6 Mev X-ray radiation dose of 3 Gy·Fx –1·d –1×5 d and β-elemene dose of 10 mg·kg –1·d –1. The average time delayed for tumor growth was obviously longer in the radiation plus β-elemene-treated group than those in the untreated group and radiation group. The SER of β-elemene was 1.89. The apoptotic index of tumor cells in the radiation plus β-elemene-treated group was also significantly higher than those in the untreated group and radiation group.

Conclusion

The β-elemene can enhance the effects of irradiation on VX2 carcinoma transplanted to kidney in rabbits in vivo by inducing apoptosis of tumor cells.

Key words: β-elemene, VX2 carcinoma transplanted in rabbits, Radiosensitization, Apoptosis

CLC Number: 

  • R737.11

Table 1

Effects of infusion of β-elemene with different doses via renal artery on growth of VX2 carcinoma transplanted in rabbits(ヌ±S, d)"

Group n Average time for enlargement of tumor Average time delayed
volume (from 3 cm3 to 10 cm3) for tumor growth
Untreated 5 18.8±1.00
β-elemene-treated
5 mg·kg–1·d–1 5 19.5±1.05 0.7±0.05
10 mg·kg–1·d–1 5 19.7±1.20 0.9±0.20
15 mg·kg–1·d–1 5 20.5±1.10* 1.7±0.10*
20 mg·kg–1·d–1 5 21.3±1.35** 2.5±0.35**

Table 2

Effects of radiation therapy (6 Mev X-ray) with different doses and infusion of β-elemene with different doses via renal artery on growth of VX2 carcinoma transplanted in rabbits(ヌ±S, d)"

Group n Average time for enlargement of tumor Average time delayed
volume (from 3 cm3 to 10 cm3) for tumor growth
Untreated 5 18.8±1.00
Radiation
1 Gy·Fx–1·d–1×5 d 5 19.2±1.00 0.4±0.00
2 Gy·Fx–1·d–1×5 d 5 20.0±1.10 1.2±0.10
3 Gy·Fx–1·d–1×5 d 5 20.9±1.30* 2.1±0.30*
4 Gy·Fx–1·d–1×5 d 5 21.4±1.89* 2.6±0.89*
5 Gy·Fx–1·d–1×5 d 5 23.6±1.78** 4.8±0.78**
Radiation plus β-elemene-treated (10 mg·kg–1·d–1)
1 Gy·Fx–1·d–1×5 d 5 19.4±1.00 0.6±0.00
2 Gy·Fx–1·d–1×5 d 5 20.7±1.15* 1.9±0.15*
3 Gy·Fx–1·d–1×5 d 5 23.0±2.13** 4.2±1.13**
4 Gy·Fx–1·d–1×5 d 5 23.9±2.20** 5.1±1.20**
5 Gy·Fx–1·d–1×5 d 5 24.5±2.01** 5.7±1.01**

Figure 1

Dose-effect curve of radiation therapy combined with β-elemene infusion via renal artery"

Figure 2

Ultrastructure of VX2 carcinoma transplanted in rabbits in different groups(×5 000)A: Untreated group; B: Radiation group; C: Radiation plus β-elemene-treated group."

[1] 程伟, 乔哲, 石涛 , 等. β-榄香烯对肾癌细胞的体外放射增敏作用[J]. 西安交通大学学报(医学版), 2004,25(2):182-185
doi: 10.3969/j.issn.1671-8259.2004.02.027
[2] Okada M, Kudo S, Miyazak O , et al. Antitumoral efficacy and pharmacokinetic properties of pirarubicin upon hepatic intra-arterial injection in the rabbit Vx2 tumor model[J]. Br J Cancer, 1995,71(3):518-524
[3] 沈瑜 . 肿瘤放射生物学(第1版)[M]. 北京: 中国医药科技出版社,2002,(第1版): 257, 420
[4] 赵健雄, 李雪峰 . 扶正抑瘤颗粒对放疗食管癌患者癌组织中CD44v6与nm23-H1表达的影响[J]. 中西医结合学报, 2004,2(4):262-264
[5] 金乐红, 刘传飞, 曾宇 . 葛根素对实验性大鼠电离辐射损伤的保护效应[J]. 中西医结合学报, 2005,3(1):43-45
[6] 周洪语, 郭建忠, 沈健康 . 榄香烯乳剂抗肿瘤作用基础研究进展[J]. 中国肿瘤临床, 2003,30(10):752-754
doi: 10.3969/j.issn.1000-8179.2003.10.023
[7] 邹丽娟, 孙秀华, 徐晓颖 , 等. 榄香烯乳剂对小鼠移植瘤U14放疗增敏的实验研究[J]. 中华放射医学与防护杂志, 2004,24(3):254-255
doi: 10.3760/cma.j.issn.0254-5098.2004.03.023
[8] 李文秀, 周梁, 金晓杰 . 榄香烯乳剂诱导喉癌细胞凋亡的实验观察[J]. 上海第二医科大学学报, 2002,22(4):306-308
doi: 10.3969/j.issn.1674-8115.2002.04.006
[1] Maged Mohamed Maher Abou-Hashem, Dina Mohamed Abo-elmatty, Noha Mostafa Mesbah, Ahmed Mohamed Abd EL-Mawgoud. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. Journal of Integrative Medicine, 2019, 17(6): 410-422.
[2] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Apirada Sucontphunt, Mattaka Khongkow, Thongchai Koobkokkruad, Nontima Vardhanabhuti. Flavonoids kaempferide and 4,2′-dihydroxy-4′,5′,6′-trimethoxychalcone inhibit mitotic clonal expansion and induce apoptosis during the early phase of adipogenesis in 3T3-L1 cells. Journal of Integrative Medicine, 2019, 17(4): 288-295.
[3] Shriniwas S. Basaiyye, Sanjay Kashyap, Kannan Krishnamurthi, Saravanadevi Sivanesan. Induction of apoptosis in leukemic cells by the alkaloid extract of garden cress (Lepidium sativum L.). Journal of Integrative Medicine, 2019, 17(3): 221-228.
[4] Carlos R. Oliveira, Daniel G. Spindola, Daniel M. Garcia, Adolfo Erustes, Alexandre Bechara, Caroline Palmeira-dos-Santos, Soraya S. Smaili, Gustavo J.S. Pereira, André Hinsberger, Ezequiel P. Viriato, Maria Cristina Marcucci, Alexandra C.H.F. Sawaya, Samantha L. Tomaz, Elaine G. Rodrigues, Claudia Bincoletto. Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development. Journal of Integrative Medicine, 2019, 17(2): 132-140.
[5] Bruno José Martins Da Silva, Sandro Wilson Gomes Pereira, Ana Paula Drummond Rodrigues, José Luiz Martins Do Nascimento, Edilene Oliveira Silva. In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. Journal of Integrative Medicine, 2018, 16(6): 404-410.
[6] Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan . Casticin from Vitex species: A short review on its anticancer and anti-inflammatory properties. Journal of Integrative Medicine, 2018, 16(3): 147-152.
[7] Jesmin Mondal, Asmita Samadder, Anisur Rahman Khuda-Bukhsh. Psorinum 6× triggers apoptosis signals in human lung cancer cells. Journal of Integrative Medicine, 2016, 14(2): 143-153.
[8] Qing-qing Zang, Lu Zhang, Ning Gao, Cheng Huang. Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells. Journal of Integrative Medicine, 2016, 14(1): 51-59.
[9] Santu Kumar Saha, Sourav Roy, Anisur Rahman Khuda-Bukhsh. Ultra-highly diluted plant extracts of Hydrastis canadensis and Marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro. Journal of Integrative Medicine, 2015, 13(6): 400-411.
[10] Samrat Ghosh, Sourav Sikdar, Avinaba Mukherjee, Anisur Rahman Khuda-Bukhsh. Evaluation of chemopreventive potentials of ethanolic extract of Ruta graveolens against A375 skin melanoma cells in vitro and induced skin cancer in mice in vivo. Journal of Integrative Medicine, 2015, 13(1): 34-44.
[11] Samrat Ghosh, Kausik Bishayee, Anisur Rahman Khuda-Bukhsh​. Oleanolic acid isolated from ethanolic extract of Phytolacca decandra induces apoptosis in A375 skin melanoma cells: Drug-DNA interaction and signaling cascade. Journal of Integrative Medicine, 2014, 12(2): 102-114.
[12] Paul Avijit, Das Sreemanti, Das Jayeeta, Samadder Asmita, Bishayee Kausik, Sadhukhan Ratan, Anisur Rahman Khuda-Bukhsh. Diarylheptanoid-myricanone isolated from ethanolic extract of Myrica cerifera shows anticancer effects on HeLa and PC3 cell lines: Signalling pathway and drug-DNA interaction. Journal of Chinese Integrative Medicine, 2013, 11(6): 405-415.
[13] Farha A. Kabeer, Geetha B. Sreedevi, Mangalam S. Nair, Dhanya S. Rajalekshmi, Latha P. Gopalakrishnan, Sujathan Kunjuraman, Remani Prathapan. Antineoplastic effects of deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, on lung adenocarcinoma (A549) cells. Journal of Chinese Integrative Medicine, 2013, 11(4): 269-277.
[14] Samrat Ghosh, Kausik Bishayee, Avijit Paul, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Homeopathic mother tincture of Phytolacca decandra induces apoptosis in skin melanoma cells by activating caspase-mediated signaling via reactive oxygen species elevation. Journal of Chinese Integrative Medicine, 2013, 11(2): 116-124.
[15] Avijit Paul, Kausik Bishayee, Samrat Ghosh, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Chelidonine isolated from ethanolic extract of Chelidonium majus promotes apoptosis in HeLa cells through p38-p53 and PI3K/AKT signalling pathways. Journal of Chinese Integrative Medicine, 2012, 10(9): 1025-1038.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[2] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[3] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
[4] Mei-jun Lü, Hui-yong Zhang, Zhe Zhang, Guan-lin Yang. Measurement of health-related quality of life in coronary heart disease: A review. Journal of Chinese Integrative Medicine, 2011, 9(12): 1277-1285
[5] Shuo Zhang , Hong-cai Shang. Correlation between traditional Chinese medicine syndromes and omics: A literature review. Journal of Chinese Integrative Medicine, 2011, 9(12): 1286-1291
[6] Qin-hui Fu, Jian Pei, Jian-rong Hui, Yi Song . Studies on dynamic changes in traditional Chinese medicine syndrome patterns for stroke using data-driven and model-driven approaches: A review. Journal of Chinese Integrative Medicine, 2011, 9(12): 1292-1300
[7] Jia Guo, Li-na Wang, Dong Li. Exploring the effects of Chinese medicine in improving uterine endometrial blood flow for increasing the successful rate of in vitro fertilization and embryo transfer. Journal of Chinese Integrative Medicine, 2011, 9(12): 1301-1306
[8] Liang-ping Hu, Xiao-lei Bao, Xue Guan, Shi-guo Zhou. Estimation of sample size and testing power (Part 3). Journal of Chinese Integrative Medicine, 2011, 9(12): 1307-1311
[9] Jian-sheng Li, Su-yun Li, Xue-qing Yu, Yang Xie. Bufei Yishen Granule combined with acupoint-sticking therapy in patients with stable chronic obstructive pulmonary disease: Study protocol of a multicenter, randomized, double-blind, active-controlled trial. Journal of Chinese Integrative Medicine, 2011, 9(12): 1312-1318
[10] Se-Der Wu, Jen-Tzer Gau, Yuh-Hai Wang. Ryodoraku as a tool monitoring the effects of walking exercise. Journal of Chinese Integrative Medicine, 2011, 9(12): 1319-1325