β-榄香烯介入治疗兔VX2肾移植癌的实验研究

doi:10.3736/jcim20060614

Abstract

Objective

To investigate the curative effect and mechanism of β-elemene interventional treatment on VX2 carcinoma transplanted on kidney in rabbits.

Methods

The rabbits were all transplanted with VX2 carcinoma on kidney. Fifty-five rabbits were randomly divided into 11 groups. Rabbits in these groups were administered interventional treatment of normal saline, iodinated oil, mitomycin, 5-fluorouracil, β-elemene, cisplatin, carboplatin, adriamycin, thiotepa, cyclophopsphamide, and vincristine, respectively. After corresponding intervention, the tumor volume in each group was measured by ultrasonography and spiral computed tomography, and the tumor growth rate (TGR) was calculated. Nenal and hepatic functions of the rabbits in each group were compared 1 day, 7 and 14 days after the interventional treatment. Morphologic change of the tumor was observed by a light microscopy and a transmission electron microscopy 14 days after interventional treatment. The expressions of Bax and Bcl-2 were measured by immunohistochemical straining.

Results

There was statistical significance in the effects of different medicines intervened on VX2 kidney transplanted carcinoma. The VX2 carcinoma of rabbits had high-sensitivity to iodized oil embolism, mitomycin, cisplatin and carboplatin, which showed serious damage to the kidney function, medium-sensitivity to β-elemene, adriamycin and 5-fluorouracil, in which β-elemene showed slight damage to the kidney function, and resistance to thiotepa, cyclohosphamide and vincristine. Most tumor cells displayed apoptosis in the β-elemene interventional treatment group under light microscopy and transmission electron microscopy, and only few tumor cells displayed necrosis. The Bax expression was up-regulated (P<0.05) and the Bcl-2 expression had no significant difference (P>0.05) in the β-elemene interventional treatment group.

Conclusion

Intervention treatment of β-elemene has significant effect on VX2 kidney transplanted carcinoma and little side effect on the kidney function. Its mechanism is related to enhancing the apoptosis of tumor cells, and Bax gene participates in this action.

Key words: β-elemene, VX2 carcinoma transplanted on kidney in rabbits, Interventional treatment, Apoptosis

CLC Number:

R737.11

Jun-jun She, Zi-ming Wang, Xiang-ming Che, Cheng-en Pan. Research of β-elemene interventional treatment on VX2 carcinoma transplanted on kidney in rabbits. Journal of Chinese Integrative Medicine, 2006, 4(6): 611-614.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20060614

http://www.jcimjournal.com/EN/Y2006/V4/I6/611

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References 7

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Research of β-elemene interventional treatment on VX2 carcinoma transplanted on kidney in rabbits

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