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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (1): 50-55.doi: 10.3736/jcim20070110

• Original Experimental Research • Previous Articles     Next Articles

Effects of Gymnadenia conopsea alcohol extract on collagen synthesis in rat lungs exposed to silica and its mechanism of antioxidative stress

Jun Wang, Jing-Bo Zeng, Xue-Feng Zhao, Qian Li, Shi-Xin Wang   

  1. Department of Scientific Research, Medical College of Chinese People's Armed Police Force, Tianjin 300162, China
  • Online:2007-01-31 Published:2007-01-21

Objective: To explore the effects of Gymnadenia conopsea alcohol extract (GcAE) on the collagen synthesis in rat lungs exposed to silica and the influence on antioxidase activities, level of lipid peroxidation (LPO).

Methods: One hundred and twenty rats were randomly divided into control group, silica group, and GcAE-treated group. Silicotic animal models were established by direct tracheal instillation of silica into rat lungs surgically. From the second day of model establishment, rats in GcAE-treated group were orally given GcAE [8 g/(kg·d) corresponding to raw herb]. At 7, 14, 21, 28 and 60 days after establishment of the animal model, eight rats in each group were sacrificed, and samples were collected. The malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in plasma were assayed by a spectrophotometer. Types Ⅰ and Ⅲ collagen were detected by Sirius red polarization and microscopy, and measuered by Image-Pro Plus Version 4.5 for Windows software.

Results: GcAE could reduce the lung/body weight ratio of rats exposed to silica, the synthesis of types ⅠandⅢcollagen of the lungs and the level of lipid peroxidation, increase the activities of SOD and GPx.

Conclusion: GcAE can ameliorate the silica-induced pulmonary fibrosis by increasing the activities of antioxidase and alleviating the damage of lipid peroxidation to the lungs.

Key words: silicosis, collagen, lipid peroxidation, pulmonary fibrosis

CLC Number: 

  • R563

Table 1

Effects of GcAE on lung/body weight ratio of rats exposed to silica at different time points (?$\bar{x}±S$m,g/g)"

Group n Lung/body weight ratio at different time points
7th day 14th day 21st day 28th day 60th day
Control 8 6.61±0.87 6.06±1.22 6.76±1.08 6.86±0.42 5.26±0.50
Silica 8 11.04±1.52** 9.28±2.70** 9.11±1.20** 11.98±2.93** 13.91±4.13**
GcAE-treated 8 8.60±0.59 6.99±1.18**△△ 7.25±1.46** 7.97±1.27** 9.75±1.58**

Figure 1

Results of HE staining for lungs of rats exposed to silica at different time points (HE staining, ×100)A: 7 d, control group; B: 7 d,silica group; C: 7 d,GcAE-treated group; D:14 d, control group; E: 14 d, silica group; F: 14 d, GcAE-treated group; G: 21 d, control group; H: 21 d, silica group; I: 21 d, GcAE-treated group; J: 28 d, control group; K: 28 d, silica group; L: 28 d, GcAE-treated group; M: 60 d, control group; N: 60 d, silica group; O: 60 d, GcAE-treated group."

Figure 2

Effects of GcAE on the synthesis of types Ⅰand Ⅲ collagen of lungs of rats exposed to silica at different time points (Sirius red staining, ×200)A: 7 d, control group; B: 7 d, silica group; C: 7 d, GcAE-treated group; D: 14 d, control group; E: 14 d, silica group; F: 14 d, GcAE-treated group; G: 21 d, control group; H: 21 d, silica group; I: 21 d, GcAE-treated group; J: 28 d, control group; K: 28 d, silica group; L: 28 d, GcAE-treated group; M: 60 d, control group; N: 60 d, silica group; O: 60 d, GcAE-treated group."

Table 2

Effects of GcAE on type Ⅰ collagen positive area percent of lung in rats exposed to silica at different time points ($\bar{x}±S$,%)"

Group n Type Ⅰ collagen positive area percentage of lung at different time points
7th day 14th day 21st day 28th day 60th day
Control 8 44.44±1.49 46.81±0.81 46.21±1.04 46.31±1.23 46.63±0.89
Silica 8 85.56±1.65** 86.32±1.29** 82.84±0.83** 107.58±0.84** 111.28±1.62**
GcAE-treated 8 65.35±1.39*△△ 66.40±1.52*△△ 72.67±1.68*△△ 84.51±0.59*△△ 86.88±0.69*△△

Table 3

Effects of GcAE on type Ⅲ collagen positive area percent of lung in rats exposed to silica at different time points ($\bar{x}±S$,%)"

Group n Type Ⅲ collagen positive area percentage of lung at different time points
7th day 14th day 21st day 28th day 60th day
Control 8 46.15±1.12 46.82±1.21 47.73±0.73 46.94±1.07 45.81±0.87
Silica 8 49.22±0.81* 64.40±0.91* 75.55±0.94* 50.31±0.88 47.78±0.78
GcAE-treated 8 48.20±1.09 51.98±1.03 63.11±1.16* 49.40±0.73 46.85±1.06

Table 4

Effects of GcAE on MDA content in plasma from rats exposed to silica at different time points ($\bar{x}±S$,mol/L)"

Group n MDA content in plasma at different time points
7th day 14th day 21st day 28th day 60th day
Control 8 3.50±0.75 3.57±0.57 4.19±1.40 3.34±0.85 3.76±0.74
Silica 8 6.43±0.85** 6.88±1.29** 6.98±1.24** 6.12±1.26** 5.82±1.16*
GcAE-treated 8 4.78±0.47*△△ 5.39±0.47* 5.48±1.35* 5.29±0.75* 4.35±1.15

Table 5

Effects of GcAE on SOD activity in plasma from rats exposed to silica at different time points ($\bar{x}±S$,kU/L)"

Group n SOD activity in plasma at different time points
7th day 14th day 21st day 28th day 60th day
Control 8 292.53±40.63 288.11±54.32 294.09±56.21 288.00±25.69 278.50±23.14
Silica 8 314.84±28.89 183.62±26.31** 219.41±40.39* 218.44±30.48** 226.50±47.86*
GcAE-treated 8 302.67±12.95 243.95±37.79△△ 293.38±49.99 277.74±29.16 243.36±41.39

Table 6

Effects of GcAE on GPx activity in plasma from rats exposed to silica at different time points ($\bar{x}±S$,U/L)"

Group n GPx activity in plasma at different time points
7th day 14th day 21st day 28th day 60th day
Control 8 2 266.67±341.61 2 018.07±240.72 2 214.01±171.66 2 237.08±207.82 2 196.64±249.05
Silica 8 2 489.50±191.97 1 015.34±221.43** 1 227.83±81.77** 1 814.90±345.15* 1 876.38±304.79
GcAE-treated 8 2 199.58±235.53 2 359.34±221.43△△ 2 538.66±189.47△△ 2 422.41±587.15 2 298.04±277.42
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