手掌参醇提物对染矽尘大鼠肺组织胶原合成的影响及其抗氧化应激机制

doi:10.3736/jcim20070110

Abstract

Objective: To explore the effects of Gymnadenia conopsea alcohol extract (GcAE) on the collagen synthesis in rat lungs exposed to silica and the influence on antioxidase activities, level of lipid peroxidation (LPO).

Methods: One hundred and twenty rats were randomly divided into control group, silica group, and GcAE-treated group. Silicotic animal models were established by direct tracheal instillation of silica into rat lungs surgically. From the second day of model establishment, rats in GcAE-treated group were orally given GcAE [8 g/（kg·d） corresponding to raw herb]. At 7, 14, 21, 28 and 60 days after establishment of the animal model, eight rats in each group were sacrificed, and samples were collected. The malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in plasma were assayed by a spectrophotometer. Types Ⅰ and Ⅲ collagen were detected by Sirius red polarization and microscopy, and measuered by Image-Pro Plus Version 4.5 for Windows software.

Results: GcAE could reduce the lung/body weight ratio of rats exposed to silica, the synthesis of types ⅠandⅢcollagen of the lungs and the level of lipid peroxidation, increase the activities of SOD and GPx.

Conclusion: GcAE can ameliorate the silica-induced pulmonary fibrosis by increasing the activities of antioxidase and alleviating the damage of lipid peroxidation to the lungs.

Key words: silicosis, collagen, lipid peroxidation, pulmonary fibrosis

CLC Number:

R563

Jun Wang, Jing-Bo Zeng, Xue-Feng Zhao, Qian Li, Shi-Xin Wang. Effects of Gymnadenia conopsea alcohol extract on collagen synthesis in rat lungs exposed to silica and its mechanism of antioxidative stress. Journal of Chinese Integrative Medicine, 2007, 5(1): 50-55.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20070110

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Group	n	Lung/body weight ratio at different time points
Group	n	7th day	14th day	21st day	28th day	60th day
Control	8	6.61±0.87	6.06±1.22	6.76±1.08	6.86±0.42	5.26±0.50
Silica	8	11.04±1.52**	9.28±2.70**	9.11±1.20**	11.98±2.93**	13.91±4.13**
GcAE-treated	8	8.60±0.59	6.99±1.18**^△△	7.25±1.46**^△	7.97±1.27**^△	9.75±1.58**^△

Group	n	Type Ⅰ collagen positive area percentage of lung at different time points
Group	n	7th day	14th day	21st day	28th day	60th day
Control	8	44.44±1.49	46.81±0.81	46.21±1.04	46.31±1.23	46.63±0.89
Silica	8	85.56±1.65**	86.32±1.29**	82.84±0.83**	107.58±0.84**	111.28±1.62**
GcAE-treated	8	65.35±1.39*^△△	66.40±1.52*^△△	72.67±1.68*^△△	84.51±0.59*^△△	86.88±0.69*^△△

Group	n	Type Ⅲ collagen positive area percentage of lung at different time points
Group	n	7th day	14th day	21st day	28th day	60th day
Control	8	46.15±1.12	46.82±1.21	47.73±0.73	46.94±1.07	45.81±0.87
Silica	8	49.22±0.81*	64.40±0.91*	75.55±0.94*	50.31±0.88	47.78±0.78
GcAE-treated	8	48.20±1.09	51.98±1.03^△	63.11±1.16*^△	49.40±0.73	46.85±1.06

Group	n	MDA content in plasma at different time points
Group	n	7th day	14th day	21st day	28th day	60th day
Control	8	3.50±0.75	3.57±0.57	4.19±1.40	3.34±0.85	3.76±0.74
Silica	8	6.43±0.85**	6.88±1.29**	6.98±1.24**	6.12±1.26**	5.82±1.16*
GcAE-treated	8	4.78±0.47*^△△	5.39±0.47*^△	5.48±1.35*^△	5.29±0.75*^△	4.35±1.15

Group	n	SOD activity in plasma at different time points
Group	n	7th day	14th day	21st day	28th day	60th day
Control	8	292.53±40.63	288.11±54.32	294.09±56.21	288.00±25.69	278.50±23.14
Silica	8	314.84±28.89	183.62±26.31**	219.41±40.39*	218.44±30.48**	226.50±47.86*
GcAE-treated	8	302.67±12.95	243.95±37.79^△△	293.38±49.99^△	277.74±29.16^△	243.36±41.39

Effects of Gymnadenia conopsea alcohol extract on collagen synthesis in rat lungs exposed to silica and its mechanism of antioxidative stress

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