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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (1): 70-73.doi: 10.3736/jcim20070114

Special Issue: Traditional Chinese Medicine

• Original Experimental Research • Previous Articles     Next Articles

An experimental serum pharmacological study on an application method in traditional Chinese medicine for treatment and prevention of asthma

Hui-feng Shen1, Yi-qun Li1, Ruo-min Jin2, He Li1, Liang Min1   

  1. 1. Department of Traditional Chinese Medicine, Renji Hospital, Medical College of Shanghai Jiao Tong University, Shanghai 200127, China
    2. Pharmacology and Safety Evaluation Laboratory, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Online:2007-01-31 Published:2007-01-21

Objective: To study the mechanism of herbal application along meridians for treatment and prevention of asthma by using serum pharmacological test to observe the effects of serum containing herbs against the constriction of tracheal spiral strips induced by acetylcholine chloride (Ach).

Methods: Guinea pigs were randomly divided into normal control group, normal saline (NS) application group, aminophylline application group, aminophylline injection group, 1-day herb application group, 7-day herb application group and 14-day herb application group. Asthma was induced by Hutson's method in guinea pigs except the normal control group. Guinea pigs in herb application groups were treated by external application of a compound herbal medicine 60 min once every day. Guinea pigs in NS application group were treated by external application of NS. Guinea pigs in the two aminophylline-treated groups were treated by external application and intraperitoneal injection of aminophylline at a dose of 400 mg/kg, respectively. The guinea pigs were killed and the sera were obtained after 1-day, 7-day and 14-day treatment in the three herb application groups, 7-day treatment in the NS application group, the aminophylline application and injection groups, respectively. Serum pharmacological method was used to do the experiment, the effects of different sera on the constriction of tracheal strips were observed, and the constriction rates were calculated.

Result: The serum containing herbs had an effect in reducing the constriction of tracheal spiral strips induced by Ach, and the effect was similar to that of the serum obtained from the aminophylline injection group. The constriction rate of the tracheal spiral strips was decreased when herbal application treatment was prolonged within a period of time, and it became stable when herbal application treatment was between 7-14 days. The constriction of tracheal spiral strips induced by Ach could be reduced remarkably when it was previously treated by serum containing herbs. 

Conclusion: The anti-acetylcholine function with a time-dependent effect is one of the mechanisms of herbal application treatment along meridians for asthma, and furthermore, herbal application treatment along meridians might be useful for preventing asthma.

Key words: asthma, Chinese herbal drugs, acetylcholine chloride, aminophylline

CLC Number: 

  • R56
[1] 蒋毅萍, 杨迎暴 . 离体气管法实验对理解平滑肌生理特性的作用与意义[J]. 第一军医大学分校学报, 2001,24(2):109-110, 112
Jiang YP, Yang YB . Effect and significance of isolated trachea experiment for students to understand the physiological properties of smooth muscle[J]. Di Yi Jun Yi Da Xue Fen Xiao Xue Bao, 2001,24(2):109-110, 112
[2] Hutson PA, Church MK, Clay TP , et al. Early and late-phase bronchoconstriction after allergen challenge of nonanesthetized guinea pigs[J]. Am Rev Respir Dis, 1988,137(3):548-557
[3] 李明华, 殷凯生, 朱栓力 , 等 .哮喘病学( 第1版)[M]. 北京: 哮喘病学, 1998,( 第1版):162
Li MH, Yin KS, Zhu SL , et al. Asthma(1st ed)[M]. Beijing: People's Medical Publishing House, 1998,( 1st ed):162
[4] 吕宝璋, 卢建, 安明榜 .受体学 ( 第2版)[M]. 合肥: 安徽科学技术出版社, 2000,( 第2版):59
Lu BZ, Lu J, An MB . Receptorology(1st ed)[M]. Hefei: Anhui Scientific and Technical Publishers, 2000,( 1st ed):59
[5] 沈惠风, 闵亮, 李鹤 , 等. 中药透皮治疗对哮喘豚鼠肺组织受体调控作用实验研究[J]. 云南中医学院学报, 2006,29(1):28-30
Shen HF, Min L, Li H , et al. Research of experiment on effect of herbal penetration therapy on receptor expression in the lung tissue of guinea pigs with experimental allergic asthma[J]. Yunnan Zhong Yi Xue Yuan Xue Bao, 2006,29(1):28-30
[6] 李福凤, 李果刚, 吴友忠 , 等. 小儿哮喘花剥苔产生的免疫机制研究[J]. 中西医结合学报, 2005,3(6):446-449
Li FF, Li GG, Wu YZ , et al. Immunological mechanism of exfoliative tongue fur in children with asthma[J]. Zhong Xi Yi Jie He Xue Bao, 2005,3(6):446-449
[7] 李昌辉, 马忠森 . 乙酰胆碱对豚鼠支气管平滑肌细胞膜钙、钾通道的影响[J]. 中国老年学杂志, 2004,24(12):1187-1188
Li CH, Ma ZS . Effects of acetylcholine on Ca2+ and K+ channels in bronchial smooth muscle cells of guinea-pig[J]. Zhongguo Lao Nian Xue Za Zhi, 2004,24(12):1187-1188
[8] 王忠慈, 马忠森, 周及红 , 等. 哮喘豚鼠模型支气管平滑肌钙通道特性的变化[J]. 中华结核和呼吸杂志, 2001,24(9):539-541
Wang ZC, Ma ZS, Zhou JH , et al. Changes of Ca2+ channel behaviors in bronchial smooth muscle cells of asthma guinea-pig model[J]. Zhonghua Jie He He Hu Xi Za Zhi, 2001,24(9):539-541
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