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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (12): 1275-1279.doi: 10.3736/jcim20081213

• Original Experimental Research • Previous Articles     Next Articles

Effects of sinomenine on synovial fluid and serum content of interleukin-1β in rabbits with osteoarthritis

Hui-qin Yang(), Li-rong Chen   

  1. Department of Rheumatology and Immunology, Wuhan No. 1 Hospital, Wuhan, Hubei Province 430022, China
  • Received:2008-07-21 Online:2008-12-20 Published:2008-12-15

Objective: 

To investigate the effects of intra-articular injection of sinomenine on interleukin-1β (IL-1β) content in synovial fluid and serum in rabbits with osteoarthritis (OA).


Methods: 

Degenerative osteoarthritis in the knee joint of left posterior limb was induced in 36 rabbits by full extension using plaster cast for 6 weeks. Then the rabbits were randomly divided into untreated group, hyaluronic acid group and sinomenine group. Another 4 normal rabbits were selected as normal control group. Rabbits in the sinomenine group and the hyaluronic acid group received intraarticular injections of sinomenine and hyaluronic acid once a week for 5 weeks, respectively. The content of IL-1β in synovial fluid and serum were measured before and after treatment by enzyme-linked immunosorbent assay. The pathological changes of cartilaginous tissue were analyzed by using Mankin's score.


Results: 

Compared with those in the untreated group, synovial fluid and serum IL-1β contents in the sinomenine group and the hyaluronic acid group were significantly decreased (P<0.01). And the synovial fluid and serum IL-1β contents in the sinomenine group were lower than those in the hyaluronic acid group (P<0.05). The mean Mankin's score of cartilage in the sinomenine group was significantly lower than that in hyaluronic acid group (P<0.05).


Conclusion: 

Sinomenine may reduce the degree of articular degeneration in rabbit with OA through decreasing the content of IL-1β in synovial fluid and serum.

Key words: osteoarthritis, sinomenine, interleukin-β, rabbits

CLC Number: 

  • R684.3

Table 1

Changes of IL-1β contents in synovial fluid and serum in four groups (x±s, ng/L)"

Group n Synovial fluid IL-1β content Serum IL-1β content
Pre-treatment Post-treatment Pre-treatment Post-treatment
Normal control 4 10.26±0.02** 10.31±0.20** 7.18±0.01** 7.24±0.17**
Untreated 12 14.57±0.05 14.60±0.22 12.80±0.03 12.84±0.03
Hyaluronic acid 12 14.14±0.29 13.38±0.20** 12.55±0.38 10.55±0.17**
Sinomenine 12 14.04±0.17 11.42±0.26** 12.63±0.17 8.58±0.01**

Figure 1

Pathological sections of articular cartilage by HE staining (Light microscopy, ×100) A: Normal control group; B: Untreated group; C: Hyaluronic acid group; D: Sinomenine group."

Figure 2

Pathological sections of articular cartilage by S/F staining (Light microscopy, ×100) A: Normal control group; B: Untreated group; C: Hyaluronic acid group; D: Sinomenine group."

Table 2

Mankin's score of articular cartilage in four groups (x±s)"

Group n Mankin's score
Normal control 4 1.22±0.02**
Untreated 12 11.22±0.04
Hyaluronic acid 12 8.73±0.05**
Sinomenine 12 6.82±0.05**
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