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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (10): 963-968.doi: 10.3736/jcim20091010

• Original Experimental Research • Previous Articles     Next Articles

Inhibiting cyclooxygenase and 5-lipoxygenase activities is an anti-inflammatory mechanism of Huzhang Gout Granule

 Yi-fei Wang, Wen-jing Wu, Ming Zhang,Min Zhou, Bin Li   

  1. Gout Clinic, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2009-07-01 Accepted:2009-08-12 Online:2009-10-20 Published:2009-10-15
  • Contact: Ming Zhang


To observe the effects of Huzhang Gout Granule (HZGG), a compound traditional Chinese herbal medicine, on cyclooxygenase (COX) and 5-lipoxygenase (5-LOX) activities, the two important oxidases in the course of inflammation, so as to investigate the possible anti-inflammatory mechanism of HZGG.

After stimulating the blood sample of healthy volunteer with calcium ionophore A23187, concentration of thromboxane B2 (TXB2) in the healthy volunteer’s blood was detected by enzyme-linked immunosorbent assay (ELISA) to observe the effects of HZGG at low- and high-dose on the activity of COX-1, with aspirin as control drug. The concentration of prostaglandin I2 (PGI2) in the healthy volunteer’s blood sample, in which aspirin was added to destroy activity of COX-1 beforehand and which was stimulated with lipopolysaccharide, was detected by ELISA method to observe the effects of HZGG on the activity of COX-2, with celecoxib as control drug. In the animal experiment, 40 rats were implanted with sponges soaking in 0.5% arachidonic acid solution in the back to induce inflammatory effusion. Content of leukotriene B4 (LTB4) in the polymorphonuclear leukocytes (PMNs) from the inflammatory effusions was detected with reversed-phase high-performance liquid chromatography (RP-HPLC) to observe the impacts of different doses of HZGG on the activity of 5-LOX, with dexamethasone as control drug.

The concentration of TXB2 in the low-dose HZGG group was higher than those in the high-dose HZGG group and the aspirin group (P<0.05). The concentrations of PGI2 in the low- and high-dose HZGG groups were higher than that in the celecoxib group (P<0.05), but there was no significant difference between the low-dose HZGG group and the high-dose HZGG group (P>0.05). The content of LTB4 in the blank control group was higher than those in the low-dose HZGG group, the high-dose HZGG group or the dexamethasone group (P<0.05).

HZGG can reduce the releasing of inflammatory mediators, such as TXB2, PGI2 and LTB4, by inhibiting the activities of COX and 5-LOX.

Key words: Acute gouty arthritis, Huzhang Gout Granule, Cyclooxygenase, 5-lipoxygenase

Table 1

TXB2 concentration in plasma in different groups ($\bar{x}$±s, ng/mL)"

Group n TXB2
Blank control 6 0.59±0.07
Aspirin 14 0.31±0.05
Low-dose HZGG 13 0.46±0.12*
High-dose HZGG 14 0.39±0.04*

Table 2

PGI2 concentration in plasma in different groups ($\bar{x}$±s, ng/mL)"

Group n PGI2
Blank control 6 82.17±6.63
Celecoxib 14 35.71±8.58
Low-dose HZGG 13 55.77±14.56
High-dose HZGG 14 47.12±9.67

Table 3

Area of LTB4 in different ($\bar{x}$±s, %)"

Group n LTB4
Blank control 10 96.90±1.83
Dexamethasone 10 34.40±6.56*
Low-dose HZGG 10 76.30±5.28*
High-dose HZGG 10 64.78±6.14*△□

Figure 1

Absorbance of LTB4 in different groups observed by HPLC A: Internal standard of PGB2; B: Blank control group; C: Dexamethasone group; D: Low-dose HZGG group; E: High-dose HZGG group."

[1] Zhang M, Jin RM, Wang HM, Li B, Ning L, Zhou R, Zhou M, Gu DQ, Xu WB . Anti-inflammatory effect of Gout Infusion Preparation(GIP) on acute gouty arthritis[J]. Tianjin Zhong Yi Yao, 2003,20(4):32-35
doi: 10.3969/j.issn.1672-1519.2003.04.011
张明, 金若敏, 王红梅, 李斌, 宁炼, 周蓉, 周敏, 顾荻青, 徐文彬 . 痛风冲剂对急性痛风性关节炎抗炎作用的研究[J]. 天津中医药, 2003,20(4):32-35
doi: 10.3969/j.issn.1672-1519.2003.04.011
[2] Ning L, Jin RM, Wu YP, Zhang HG, Qi HH, Zhang M, Li B . Experimental study of Gout Granule for gout[J]. Zhong Yao Xin Yao Yu Lin Chuang Yao Li, 2003,14(1):28-30
doi: 10.3321/j.issn:1003-9783.2003.01.010
宁炼, 金若敏, 吴耀平, 张海贵, 祁海宏, 张明, 李斌 . 痛风冲剂抗痛风作用的实验研究[J]. 中药新药与临床药理, 2003,14(1):28-30
doi: 10.3321/j.issn:1003-9783.2003.01.010
[3] Tang N, Li ZY, Zhou YM, Liu BL . Evaluation of anti-inflammatory effects of luteolin and underlying mechanisems[J]. Zhongguo Lin Chuang Yao Li Xue Yu Zhi Liao Xue, 2007,12(2):195-199
doi: 10.3969/j.issn.1009-2501.2007.02.015
唐宁, 李子艳, 周咏梅, 刘保林 . 木樨草素抗炎作用机制的初步探讨[J]. 中国临床药理学与治疗学, 2007,12(2):195-199
doi: 10.3969/j.issn.1009-2501.2007.02.015
[4] Lu Y, He JY, Chen DC, Xuan GW . Experimental study on inhibition of LTB4 production of mizolastin[J]. Lin Chuang Pi Fu Ke Za Zhi, 2005,34(11):738-739
doi: 10.3969/j.issn.1000-4963.2005.11.008
陆原, 何敬远, 陈达灿, 禤国维 . 咪唑斯汀抑制多形核白细胞白三烯LTB4合成的实验研究[J]. 临床皮肤科杂志, 2005,34(11):738-739
doi: 10.3969/j.issn.1000-4963.2005.11.008
[5] Martel-Pelletier J, Pelletier JP, Fahmi H . Cyclooxygenase-2 and prostaglandins in articular tissues. Semin Arthritis Rheum[J]., 2003,33(3):137-139
[6] Fernandes JC, Martel-Pelletier J, Pelletier JP . The role of cytokines in osteoarthritis pathophysiology[J]. Biorheology, 2002,39(1-2):237-246
[7] Kagi MK . Leukotriene receptor antagonists a novel therapeutic approach in atopic dermatitis?[J]. Dermatology, 2001,203(4):280-283
doi: 10.1159/000051772 pmid: 11752812
[8] Gaddi A, Cicero AF, Pedro EJ . Clinical perspectives of anti-inflammatory therapy in the elderly: the lipoxygenase(LOX)/cyclooxygenase(COX) inhibition concept[J]. Arch Gerontol Geriatr, 2004,38(3):201-212
[9] Zhang M, Zhu Z, Wang YF . Clinical observation of "Huzhang Tongfeng Granule" in treating acute gouty arthritis[J]. Shanghai Zhong Yi Yao Za Zhi, 2008,42(6):576-580
doi: 10.3969/j.issn.1007-1334.2008.06.008
张明, 朱周, 王一飞 . 虎杖痛风颗粒治疗急性痛风性关节炎临床观察[J]. 上海中医药杂志, 2008,42(6):576-580
doi: 10.3969/j.issn.1007-1334.2008.06.008
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