从环氧化酶和5-脂氧化酶途径探讨虎杖痛风颗粒的抗炎机制

doi:10.3736/jcim20091010

Abstract

Objective

To observe the effects of Huzhang Gout Granule (HZGG), a compound traditional Chinese herbal medicine, on cyclooxygenase (COX) and 5-lipoxygenase (5-LOX) activities, the two important oxidases in the course of inflammation, so as to investigate the possible anti-inflammatory mechanism of HZGG.
Methods

After stimulating the blood sample of healthy volunteer with calcium ionophore A23187, concentration of thromboxane B2 (TXB2) in the healthy volunteer’s blood was detected by enzyme-linked immunosorbent assay (ELISA) to observe the effects of HZGG at low- and high-dose on the activity of COX-1, with aspirin as control drug. The concentration of prostaglandin I2 (PGI2) in the healthy volunteer’s blood sample, in which aspirin was added to destroy activity of COX-1 beforehand and which was stimulated with lipopolysaccharide, was detected by ELISA method to observe the effects of HZGG on the activity of COX-2, with celecoxib as control drug. In the animal experiment, 40 rats were implanted with sponges soaking in 0.5% arachidonic acid solution in the back to induce inflammatory effusion. Content of leukotriene B4 (LTB4) in the polymorphonuclear leukocytes (PMNs) from the inflammatory effusions was detected with reversed-phase high-performance liquid chromatography (RP-HPLC) to observe the impacts of different doses of HZGG on the activity of 5-LOX, with dexamethasone as control drug.
Results

The concentration of TXB2 in the low-dose HZGG group was higher than those in the high-dose HZGG group and the aspirin group (P<0.05). The concentrations of PGI2 in the low- and high-dose HZGG groups were higher than that in the celecoxib group (P<0.05), but there was no significant difference between the low-dose HZGG group and the high-dose HZGG group (P>0.05). The content of LTB4 in the blank control group was higher than those in the low-dose HZGG group, the high-dose HZGG group or the dexamethasone group (P<0.05).
Conclusion

HZGG can reduce the releasing of inflammatory mediators, such as TXB2, PGI2 and LTB4, by inhibiting the activities of COX and 5-LOX.

Key words: Acute gouty arthritis, Huzhang Gout Granule, Cyclooxygenase, 5-lipoxygenase

Yi-fei Wang, Wen-jing Wu, Ming Zhang, Min Zhou, Bin Li. Inhibiting cyclooxygenase and 5-lipoxygenase activities is an anti-inflammatory mechanism of Huzhang Gout Granule. Journal of Chinese Integrative Medicine, 2009, 7(10): 963-968.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20091010

http://www.jcimjournal.com/EN/Y2009/V7/I10/963

TrendMD

Figures/Tables 4

Table 1

Table 2

Table 3

Figure 1

References 9

[1]	Zhang M, Jin RM, Wang HM, Li B, Ning L, Zhou R, Zhou M, Gu DQ, Xu WB . Anti-inflammatory effect of Gout Infusion Preparation(GIP) on acute gouty arthritis[J]. Tianjin Zhong Yi Yao, 2003,20(4):32-35 doi: 10.3969/j.issn.1672-1519.2003.04.011
	张明, 金若敏, 王红梅, 李斌, 宁炼, 周蓉, 周敏, 顾荻青, 徐文彬 . 痛风冲剂对急性痛风性关节炎抗炎作用的研究[J]. 天津中医药, 2003,20(4):32-35 doi: 10.3969/j.issn.1672-1519.2003.04.011
[2]	Ning L, Jin RM, Wu YP, Zhang HG, Qi HH, Zhang M, Li B . Experimental study of Gout Granule for gout[J]. Zhong Yao Xin Yao Yu Lin Chuang Yao Li, 2003,14(1):28-30 doi: 10.3321/j.issn:1003-9783.2003.01.010
	宁炼, 金若敏, 吴耀平, 张海贵, 祁海宏, 张明, 李斌 . 痛风冲剂抗痛风作用的实验研究[J]. 中药新药与临床药理, 2003,14(1):28-30 doi: 10.3321/j.issn:1003-9783.2003.01.010
[3]	Tang N, Li ZY, Zhou YM, Liu BL . Evaluation of anti-inflammatory effects of luteolin and underlying mechanisems[J]. Zhongguo Lin Chuang Yao Li Xue Yu Zhi Liao Xue, 2007,12(2):195-199 doi: 10.3969/j.issn.1009-2501.2007.02.015
	唐宁, 李子艳, 周咏梅, 刘保林 . 木樨草素抗炎作用机制的初步探讨[J]. 中国临床药理学与治疗学, 2007,12(2):195-199 doi: 10.3969/j.issn.1009-2501.2007.02.015
[4]	Lu Y, He JY, Chen DC, Xuan GW . Experimental study on inhibition of LTB4 production of mizolastin[J]. Lin Chuang Pi Fu Ke Za Zhi, 2005,34(11):738-739 doi: 10.3969/j.issn.1000-4963.2005.11.008
	陆原, 何敬远, 陈达灿, 禤国维 . 咪唑斯汀抑制多形核白细胞白三烯LTB4合成的实验研究[J]. 临床皮肤科杂志, 2005,34(11):738-739 doi: 10.3969/j.issn.1000-4963.2005.11.008
[5]	Martel-Pelletier J, Pelletier JP, Fahmi H . Cyclooxygenase-2 and prostaglandins in articular tissues. Semin Arthritis Rheum[J]., 2003,33(3):137-139
[6]	Fernandes JC, Martel-Pelletier J, Pelletier JP . The role of cytokines in osteoarthritis pathophysiology[J]. Biorheology, 2002,39(1-2):237-246
[7]	Kagi MK . Leukotriene receptor antagonists a novel therapeutic approach in atopic dermatitis?[J]. Dermatology, 2001,203(4):280-283 doi: 10.1159/000051772 pmid: 11752812
[8]	Gaddi A, Cicero AF, Pedro EJ . Clinical perspectives of anti-inflammatory therapy in the elderly: the lipoxygenase(LOX)/cyclooxygenase(COX) inhibition concept[J]. Arch Gerontol Geriatr, 2004,38(3):201-212
[9]	Zhang M, Zhu Z, Wang YF . Clinical observation of "Huzhang Tongfeng Granule" in treating acute gouty arthritis[J]. Shanghai Zhong Yi Yao Za Zhi, 2008,42(6):576-580 doi: 10.3969/j.issn.1007-1334.2008.06.008
	张明, 朱周, 王一飞 . 虎杖痛风颗粒治疗急性痛风性关节炎临床观察[J]. 上海中医药杂志, 2008,42(6):576-580 doi: 10.3969/j.issn.1007-1334.2008.06.008

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2]	Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3]	Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4]	Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5]	A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H₂₂ cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6]	Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7]	Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8]	Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9]	Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10]	Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022

Group	n	TXB2
Blank control	6	0.59±0.07
Aspirin	14	0.31±0.05
Low-dose HZGG	13	0.46±0.12*
High-dose HZGG	14	0.39±0.04*^△

Group	n	PGI2
Blank control	6	82.17±6.63
Celecoxib	14	35.71±8.58
Low-dose HZGG	13	55.77±14.56^▲
High-dose HZGG	14	47.12±9.67^▲

Group	n	LTB4
Blank control	10	96.90±1.83
Dexamethasone	10	34.40±6.56*^△
Low-dose HZGG	10	76.30±5.28*^□
High-dose HZGG	10	64.78±6.14*^△□

Inhibiting cyclooxygenase and 5-lipoxygenase activities is an anti-inflammatory mechanism of Huzhang Gout Granule

RichHTML

PDF (PC)

Like

Abstract

Cite this article

Share this article