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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (6): 568-574.doi: 10.3736/jcim20100610

• Original Experimental Research • Previous Articles     Next Articles

Effects of Bawei Xilei San on mice with oxazolone-induced colitis and the mechanisms

Yan Pan, Qin Ouyang   

  1. Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2010-01-20 Accepted:2010-03-31 Online:2010-06-20 Published:2010-06-15
  • Contact: Qin Ouyang E-mail:qin.ouyang@163.com

Objective

To investigate the therapeutic effects of Bawei Xilei San (BWXLS), a compound traditional Chinese herbal medicine, on mice with oxazolone-induced colitis and to explore the mechanisms.
Methods

Thirty-two BALB/c mice were randomly divided into 4 groups (8 for each): normal control group, untreated group, hydrocortisone group and BWXLS group. Except for the mice in the normal control group, all mice were intrarectally administered with 3.0% oxazolone to induce colitis. Then the mice in the normal control group and untreated group were administered with 0.9% carboxymethyl cellulose sodium solution. Mice in the BWXLS group were intrarectally administered with 0.2 mg/g BWXLS and hydrocortisone group with 0.02 mg/g respectively for 5 days. The body weight and stool consistency and occult or gross blood were recorded to calculate the disease activity index (DAI). The mice were sacrificed at the 6th day. The macroscopic and histological changes of the colon were evaluated. The expressions of Toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB), and epithelial tight junction protein occludin were assessed by immunohistochemical method. The level of tumor necrosis factor-α (TNF-α) in colonic mucosa was evaluated by enzyme-linked immunosorbent assay.
Results

The DAI, and macroscopic and histological changes in the BWXLS group were improved as compared with those in the untreated group (P<0.05) but were similar to those in the hydrocortisone group. The expression of occludin was significantly increased (P<0.05) while the expressions of TLR4, NF-κB and TNF-α were significantly decreased in the BWXLS group as compared with the untreated group, and were similar to those in the hydrocortisone group (P>0.05).
Conclusion

Up-regulating the expression of occludin and down-regulating the expressions of TLR4 and NF-κB, and hence inhibiting TNF-α expression and improving the mucosa barrier function may be part of the mechanisms of BWXLS in treating oxazolone-induced colitis in mice.

Key words: Compound (TCD), Oxazolone, Colitis, Mice

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Figure 1

Disease activity index of mice in different groups"

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Figure 2

Histological changes of colon in mice in different groups observed by HE staining (Light microscopy, ×400)A: Normal group; B: Untreated group; C: Hydrocortisone group; D: Bawei Xilei San group."

Figure 3

Expression of occludin in colonic mucosa of mice in different groups observed by immunohistochemical method (Light microscopy, ×400)A: Normal group; B: Untreated group; C: Hydrocortisone group; D: Bawei Xilei San group."

Figure 4

Expression of TLR4 in colonic mucosa of mice in different groups observed by immunohistochemical method (Light microscopy, ×400)A: Normal group; B: Untreated group; C: Hydrocortisone group; D: Bawei Xilei San group."

Figure 5

Expression of NF-κB in colonic mucosa of mice in different groups observed by immunohistochemical method (Light microscopy, ×400)A: Normal group; B: Untreated group; C: Hydrocortisone group; D: Bawei Xilei San group."

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