八味锡类散对小鼠恶唑酮结肠炎的治疗作用及其机制

doi:10.3736/jcim20100610

Abstract

Objective

To investigate the therapeutic effects of Bawei Xilei San (BWXLS), a compound traditional Chinese herbal medicine, on mice with oxazolone-induced colitis and to explore the mechanisms.
Methods

Thirty-two BALB/c mice were randomly divided into 4 groups (8 for each): normal control group, untreated group, hydrocortisone group and BWXLS group. Except for the mice in the normal control group, all mice were intrarectally administered with 3.0% oxazolone to induce colitis. Then the mice in the normal control group and untreated group were administered with 0.9% carboxymethyl cellulose sodium solution. Mice in the BWXLS group were intrarectally administered with 0.2 mg/g BWXLS and hydrocortisone group with 0.02 mg/g respectively for 5 days. The body weight and stool consistency and occult or gross blood were recorded to calculate the disease activity index (DAI). The mice were sacrificed at the 6th day. The macroscopic and histological changes of the colon were evaluated. The expressions of Toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB), and epithelial tight junction protein occludin were assessed by immunohistochemical method. The level of tumor necrosis factor-α (TNF-α) in colonic mucosa was evaluated by enzyme-linked immunosorbent assay.
Results

The DAI, and macroscopic and histological changes in the BWXLS group were improved as compared with those in the untreated group (P<0.05) but were similar to those in the hydrocortisone group. The expression of occludin was significantly increased (P<0.05) while the expressions of TLR4, NF-κB and TNF-α were significantly decreased in the BWXLS group as compared with the untreated group, and were similar to those in the hydrocortisone group (P>0.05).
Conclusion

Up-regulating the expression of occludin and down-regulating the expressions of TLR4 and NF-κB, and hence inhibiting TNF-α expression and improving the mucosa barrier function may be part of the mechanisms of BWXLS in treating oxazolone-induced colitis in mice.

Key words: Compound (TCD), Oxazolone, Colitis, Mice

Yan Pan, Qin Ouyang. Effects of Bawei Xilei San on mice with oxazolone-induced colitis and the mechanisms. Journal of Chinese Integrative Medicine, 2010, 8(6): 568-574.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20100610

http://www.jcimjournal.com/EN/Y2010/V8/I6/568

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Effects of Bawei Xilei San on mice with oxazolone-induced colitis and the mechanisms

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