八味锡类散对小鼠恶唑酮结肠炎的治疗作用及其机制

doi:10.3736/jcim20100610

Abstract

Objective

To investigate the therapeutic effects of Bawei Xilei San (BWXLS), a compound traditional Chinese herbal medicine, on mice with oxazolone-induced colitis and to explore the mechanisms.
Methods

Thirty-two BALB/c mice were randomly divided into 4 groups (8 for each): normal control group, untreated group, hydrocortisone group and BWXLS group. Except for the mice in the normal control group, all mice were intrarectally administered with 3.0% oxazolone to induce colitis. Then the mice in the normal control group and untreated group were administered with 0.9% carboxymethyl cellulose sodium solution. Mice in the BWXLS group were intrarectally administered with 0.2 mg/g BWXLS and hydrocortisone group with 0.02 mg/g respectively for 5 days. The body weight and stool consistency and occult or gross blood were recorded to calculate the disease activity index (DAI). The mice were sacrificed at the 6th day. The macroscopic and histological changes of the colon were evaluated. The expressions of Toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB), and epithelial tight junction protein occludin were assessed by immunohistochemical method. The level of tumor necrosis factor-α (TNF-α) in colonic mucosa was evaluated by enzyme-linked immunosorbent assay.
Results

The DAI, and macroscopic and histological changes in the BWXLS group were improved as compared with those in the untreated group (P<0.05) but were similar to those in the hydrocortisone group. The expression of occludin was significantly increased (P<0.05) while the expressions of TLR4, NF-κB and TNF-α were significantly decreased in the BWXLS group as compared with the untreated group, and were similar to those in the hydrocortisone group (P>0.05).
Conclusion

Up-regulating the expression of occludin and down-regulating the expressions of TLR4 and NF-κB, and hence inhibiting TNF-α expression and improving the mucosa barrier function may be part of the mechanisms of BWXLS in treating oxazolone-induced colitis in mice.

Key words: Compound (TCD), Oxazolone, Colitis, Mice

Yan Pan, Qin Ouyang. Effects of Bawei Xilei San on mice with oxazolone-induced colitis and the mechanisms. Journal of Chinese Integrative Medicine, 2010, 8(6): 568-574.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20100610

http://www.jcimjournal.com/EN/Y2010/V8/I6/568

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References 18

[1]	Baumgart DC, Carding SR . Inflammatory bowel disease: cause and immunobiology[J]. Lancet, 2007,369(9573):1627-1640 doi: 10.1016/S0140-6736(07)60750-8
[2]	Jiang XL, Cui HF . An analysis of 10 218 ulcerative colitis cases in China[J]. World J Gastroenterol, 2002,8(1):158-161 doi: 10.3748/wjg.v8.i1.158
[3]	Fukunaga K, Hida N, Ohnishi K, Ohda Y, Yoshida K, Kusaka T, Jinno Y, Nagase K, Nakamura S, Kadobayashi M, Miva H, Matsumoto TA . A suppository Chinese medicine(Xilei-san) for refractory ulcerative proctitis:a pilot clinical trial[J]. Digestion, 2007,75(2-3):146-147 doi: 10.1159/000106755
[4]	Heller F, Fuss IJ, Nieuwenhuis EE, Blumberg RS, Strober W . Oxazolone colitis, a Th2 colitis model resembling ulcerative colitis, is mediated by IL-13-producing NK-T cells[J]. Immunity, 2002,17(5):629-638 doi: 10.1016/S1074-7613(02)00453-3
[5]	Chen Q. Technology of research on pharmacology of traditional Chinese medicine(2nd ed)[M]. Beijing: People's Medical Publishing House, 2006: 1170
	陈奇 . 中药药理研究方法学(第2版)[M]. 北京: 人民卫生出版社, 2006: 1170
[6]	Kullmann F, Messmann H, Alt M, Gross V, Bocker T , Schölmerich J, Rüschoff J.Clinical and histopathological features of dextran sulfate sodium induced acute and chronic colitis associated with dysplasia in rats[J]. Int J Colorectal Dis, 2001,16(4):238-246 doi: 10.1007/s003840100311
[7]	Ekstrm GM . Oxazolone-induced colitis in rats: effects of budesonide, cyclosporin A, and 5-aminosalicylic acid[J]. Scand J Gastroenterol, 1998,33(2):174-179 doi: 10.1080/00365529850166914
[8]	Han Y, Murata Y, Itoh J, Munakata A . Effect of long-term nicotine on oxazolone-induced murine colitis model[J]. Zhonghua Xiao Hua Za Zhi, 2001,21(8):473-476
	韩英, 村田有志, 伊东重豪, 栋方昭博 . 长期应用尼古丁对恶唑酮诱导的实验性小鼠肠炎模型的影响及其机制探讨[J]. 中华消化杂志, 2001,21(8):473-476
[9]	Liu DY, Zhao HM, Lu AP . Compare of Bawei Xilei Powder with Xilei Powder[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2004,10(6):11-12
	刘端勇, 赵海梅, 吕爱平 . 八味锡类散与锡类散之鉴赏[J]. 中国中医基础医学杂志, 2004,10(6):11-12
[10]	Regueiro M, Loftus EV Jr, Steinhart AH, Cohen RD . Medical management of left-sided ulcerative colitis and ulcerative proctitis: critical evaluation of therapeutic trials[J]. Inflamm Bowel Dis, 2006,12(10):979-994 doi: 10.1097/01.mib.0000231495.92013.5e
[11]	Liu DY, Zhao HM, Zhao N, Lu C, Lu AP . Effect of Bawei Xilei powder on CD3, CD4, CD8 T-lymphocytes of rats with ulcerative colitis[J]. Zhongguo Zhong Yao Za Zhi, 2008,33(11):1301-1304
	刘端勇, 赵海梅, 赵宁, 吕诚, 吕爱平 . 八味锡类散对大鼠溃疡性结肠炎CD3, CD4, CD8 T淋巴细胞的影响[J]. 中国中药杂志, 2008,33(11):1301-1304
[12]	Sethi G, Ahn KS, Sandur SK, Lin X, Chaturvedi MM, Aggarwal BB . Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway[J]. J Biol Chem, 2006,281(33):23425-23435 doi: 10.1074/jbc.M602627200
[13]	Lopez E, Le Faou A, Borzeix S, Berland S . Stimulation of rat cutaneous fibroblasts and their synthetic activity by implants of powdered nacre(mother of pearl)[J]. Tissue Cell, 2000,32(1):95-101 doi: 10.1054/tice.1999.0091
[14]	Nusrat A, Chen JA, Foley CS, Liang TW, Tom J, Cromwell M, Quan C, Mrsny RJ . The coiled-coil domain of occludin can act to organize structural and functional elements of the epithelial tight junction[J]. J Biol Chem, 2000,275(38):29816-29822 doi: 10.1074/jbc.M002450200
[15]	Kucharzik T, Walsh SV, Chen J, Parkos CA, Nusrat A . Neutrophil transmigration in inflammatory bowel disease is associated with differential expression of epithelial intercellular junction proteins[J]. Am J Pathol, 2001,159(6):2001-2009 doi: 10.1016/S0002-9440(10)63051-9
[16]	Li RL, Chi L, Guo WF, Li QX, Xu SF, Chen WW . Tight junction protein occludin and claudin-1 expression in ulcerative colitis rats[J]. Guangzhou Zhong Yi Yao Da Xue Xue Bao, 2008,25(1):83-87, 90
	李茹柳, 迟莉, 郭文峰, 李秋霞, 徐颂芬, 陈蔚文 . 紧密连接蛋白occludin和claudin-1在大鼠溃疡性结肠炎的表达[J]. 广州中医药大学学报, 2008,25(1):83-87, 90
[17]	Xu N, Ouyang Q . Toll-like receptors, NF-κB and inflammatory bowel disease[J]. Guo Wai Yi Xue Xiao Hua Xi Ji Bing Fen Ce, 2003,23(5):264-267
	徐宁, 欧阳钦 . Toll样受体, NF-κB与炎症性肠病[J]. 国外医学: 消化系疾病分册, 2003,23(5):264-267
[18]	Abreu MT, Arnold ET, Thomas LS, Gonsky R, Zhou Y, Hu B, Arditi M . TLR4 and MD-2 expression is regulated by immune-mediated signals in human intestinal epithelial cells[J]. J Biol Chem, 2002,277(23):20431-20437 doi: 10.1074/jbc.M110333200

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Effects of Bawei Xilei San on mice with oxazolone-induced colitis and the mechanisms

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