Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (9): 883-887.doi: 10.3736/jcim20100913

• Original Experimental Research • Previous Articles     Next Articles

Effects of electroacupuncture on disorder of intestinal motility in a rat model of irritable bowel syndrome

 Zhi-jun Wanga, Wei-min Lib,c   

  1. a Division of Acupucture Analgesia, Department of Integrative Medicine,Shanghai Medical College, Fudan University, Shanghai 200032, China
    b Department of Mechanics and Engineering Science, Fudan University, Shanghai 200433, China
    c Shanghai Research Center for Acupuncture and Meridians, Shanghai 201203, China
  • Received:2010-04-15 Accepted:2010-05-13 Online:2010-09-20 Published:2010-09-15

Objective

To investigate the effects of electroacupuncture (EA) on intestinal motility disorder in rats with irritable bowel syndrome (IBS) in order to provide experimental evidence for improving clinical treatment to IBS with EA.
Methods

IBS was induced by inserting a balloon into the rectum of male Sprague-Dawley rats from their neonatal age (8th, 21st day after birth) and applying colorectal distention stimulation by inflating air into the balloon. Peristaltic wave (PW) was used to estimate intestinal motility. Rats were divided into 4 groups (n=6): normal group, untreated group, sham EA group and EA group. Acupoints of Zusanli (ST36) and Shangjuxu (ST37) in the hind limbs bilaterally were chosen for EA and sham EA treatment. Trains of dense-sparse frequencies (100 Hz and 2 Hz alternately) and intensity of 1, 2 and 3 mA (10 min for each) were applied for 30 min with each EA treatment while inserting similar needles without electrical stimulation was done as sham EA treatment. PW recorded for 30 min in normal and untreated groups respectively was taken as control values, while PW recorded after EA or sham EA treatment for 30 min was taken as responsive values.
Results

Frequency of PW was significantly increased in the untreated group as compared with that in the normal group (P<0.05), and decreased after 30-min EA treatment (P<0.05). There was no significant change in the PW of sham EA group as compared with the control group and before treatment (P>0.05).
Conclusion

These results suggest that 1) there is an abnormal increase in intestinal motility indicating an intestinal motility disorder in IBS rats; 2) EA but not sham EA can relieve such intestinal motility disorder.

Key words: Irritable bowel syndrome, Electroacupuncture, Intestinal motility disorder, Rats

Figure 1

The sketch of balloon for colorectal distention stimulation"

Figure 2

A schematic diagram of rats for peristaltic wave recording and electroacupuncture treatment"

Figure 3

Sign of normal group and diarrhea in IBS group"

Figure 4

Samples of peristaltic wave recorded in different groups of rats A: Normal group; B: Untreated group; C: Electroacupuncture group; D: Sham electroacupuncture group."

Figure 5

Total counts of peristaltic wave in 30 min recorded in different groups Data were represented as x±s, n=6. *P<0.05, vs normal group."

Table 1

Counts of peristaltic wave of rats in different groups"

[1] Ye RG, Lu ZY. Internal medicine(6th ed)[M]. Beijing: People’s Medical Publishing House, 2004,( 6th ed):423
叶任高, 陆再英 .内科学 ( 第6版)[M]. 北京: 人民卫生出版社, 2004,( 第6版):423
[2] Li FL . Electro-acupuncture treatment of 32 cases of irritable bowel syndrome[J]. Zhongguo Zhong Xi Yi Jie He Pi Wei Za Zhi, 2000,8(3):141
李芳莉 . 电针治疗肠激惹综合征32例[J]. 中国中西医结合脾胃杂志, 2000,8(3):141
[3] Yuan X . Electro-acupuncture treatment of one case of irritable bowel syndrome[J]. Shi Yong Zhong Yi Yao Za Zhi, 2005,21(2):108
doi: 10.3969/j.issn.1004-2814.2005.02.051
袁霞 . 电针治疗肠易激综合征1例[J]. 实用中医药杂志, 2005,21(2):108
doi: 10.3969/j.issn.1004-2814.2005.02.051
[4] Chen L, Liu S . High-frequency electro-acupuncture at Zusanli point promotes colonic propulsive motility in rats[J]. Shi Jie Hua Ren Xiao Hua Za Zhi, 2006,14(30):2962-2964
doi: 10.3969/j.issn.1009-3079.2006.30.012
陈兰, 刘诗 . 高频电针刺激足三里促进大鼠结肠推进运动[J]. 世界华人消化杂志, 2006,14(30):2962-2964
doi: 10.3969/j.issn.1009-3079.2006.30.012
[5] Al-Chaer ED, Kawasaki M, Pasricha PJ . A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development[J]. Gastroenterology, 2000,119(5):1276-1285
doi: 10.1053/gast.2000.19576
[6] Cui KM, Li WM, Gao X, Chung K, Chung JM, Wu GC . Electro-acupuncture relieves chronic visceral hyperalgesia in rats[J]. Neurosci Lett, 2005,376(1):20-23
doi: 10.1016/j.neulet.2004.11.018 pmid: 15694267
[7] Sato A, Sato Y, Suzuki A, Uchida S . Neural mechanisms of the reflex inhibition and excitation of gastric motility elicited by acupuncture-like stimulation in anesthetized rats[J]. Neurosci Res, 1993,18(1):53-62
doi: 10.1016/0168-0102(93)90105-Y
[8] Yin X, Ning SB . Advances in understanding the pathogenesis of irritable bowel syndrome[J]. Shi Jie Hua Ren Xiao Hua Za Zhi, 2009,17(32):3318-3323
doi: 10.3969/j.issn.1009-3079.2009.32.008
银新, 宁守斌 . 肠易激综合征发病机制的研究进展[J]. 世界华人消化杂志, 2009,17(32):3318-3323
doi: 10.3969/j.issn.1009-3079.2009.32.008
[9] Drossman DA . The functional gastrointestinal disorders and the Rome Ⅲ process[J]. Gastroenterology, 2006,130(5):1377-1390
doi: 10.1053/j.gastro.2006.03.008 pmid: 16678553
[10] Jia P, Chen G, Chen RX . Effects of electro-acupuncture of Zusanli point(ST36) at different time on derangement of gastric electric rhythm in rabbits[J]. Sichuan Zhong Yi, 2007,25(3):12-14
doi: 10.3969/j.issn.1000-3649.2007.03.007
贾萍, 陈刚, 陈日新 . 电针“足三里”穴对家兔胃电节律紊乱调整的时间效应关系研究[J]. 四川中医, 2007,25(3):12-14
doi: 10.3969/j.issn.1000-3649.2007.03.007
[1] Xiang-yu Wei, Shi-lei Luo, HuiChen, Shan-shan Liu, Zhi-gang Gong, Song-huaZhan. Functional connectivity changes during migraine treatment with electroacupuncture at Shuaigu (GB8). Journal of Integrative Medicine, 2022, 20(3): 237-243.
[2] Si-qi Tang, Yun-liang Wang, Zi-ye Xie, Yang Zhang, Yi Guo, Kang-li Gao, Tang-you Mao, Chun-e Xie, Jun-xiang Li, Xiao-yan Gao. Serum metabolic profiling of traditional Chinese medicine syndromes in patients with diarrhea-predominant irritable bowel syndrome. Journal of Integrative Medicine, 2021, 19(3): 274-281.
[3] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[4] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[5] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[6] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[7] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[8] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[9] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[10] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[11] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[12] Meng-yue Liu, Cheng-wei Wang, Zhou-peng Wu, Ning Li. Electroacupuncture for the prevention of postoperative gastrointestinal dysfunction in patients undergoing vascular surgery under general anesthesia: Study protocol for a prospective practical randomized controlled trial. Journal of Integrative Medicine, 2014, 12(6): 512-519.
[13] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[14] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[15] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022