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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (9): 883-887.doi: 10.3736/jcim20100913

• Original Experimental Research • Previous Articles     Next Articles

Effects of electroacupuncture on disorder of intestinal motility in a rat model of irritable bowel syndrome

 Zhi-jun Wanga, Wei-min Lib,c   

  1. a Division of Acupucture Analgesia, Department of Integrative Medicine,Shanghai Medical College, Fudan University, Shanghai 200032, China
    b Department of Mechanics and Engineering Science, Fudan University, Shanghai 200433, China
    c Shanghai Research Center for Acupuncture and Meridians, Shanghai 201203, China
  • Received:2010-04-15 Accepted:2010-05-13 Online:2010-09-20 Published:2010-09-15

Objective

To investigate the effects of electroacupuncture (EA) on intestinal motility disorder in rats with irritable bowel syndrome (IBS) in order to provide experimental evidence for improving clinical treatment to IBS with EA.
Methods

IBS was induced by inserting a balloon into the rectum of male Sprague-Dawley rats from their neonatal age (8th, 21st day after birth) and applying colorectal distention stimulation by inflating air into the balloon. Peristaltic wave (PW) was used to estimate intestinal motility. Rats were divided into 4 groups (n=6): normal group, untreated group, sham EA group and EA group. Acupoints of Zusanli (ST36) and Shangjuxu (ST37) in the hind limbs bilaterally were chosen for EA and sham EA treatment. Trains of dense-sparse frequencies (100 Hz and 2 Hz alternately) and intensity of 1, 2 and 3 mA (10 min for each) were applied for 30 min with each EA treatment while inserting similar needles without electrical stimulation was done as sham EA treatment. PW recorded for 30 min in normal and untreated groups respectively was taken as control values, while PW recorded after EA or sham EA treatment for 30 min was taken as responsive values.
Results

Frequency of PW was significantly increased in the untreated group as compared with that in the normal group (P<0.05), and decreased after 30-min EA treatment (P<0.05). There was no significant change in the PW of sham EA group as compared with the control group and before treatment (P>0.05).
Conclusion

These results suggest that 1) there is an abnormal increase in intestinal motility indicating an intestinal motility disorder in IBS rats; 2) EA but not sham EA can relieve such intestinal motility disorder.

Key words: Irritable bowel syndrome, Electroacupuncture, Intestinal motility disorder, Rats

Figure 1

The sketch of balloon for colorectal distention stimulation"

Figure 2

A schematic diagram of rats for peristaltic wave recording and electroacupuncture treatment"

Figure 3

Sign of normal group and diarrhea in IBS group"

Figure 4

Samples of peristaltic wave recorded in different groups of rats A: Normal group; B: Untreated group; C: Electroacupuncture group; D: Sham electroacupuncture group."

Figure 5

Total counts of peristaltic wave in 30 min recorded in different groups Data were represented as x±s, n=6. *P<0.05, vs normal group."

Table 1

Counts of peristaltic wave of rats in different groups"

[1] Ye RG, Lu ZY. Internal medicine(6th ed)[M]. Beijing: People’s Medical Publishing House, 2004,( 6th ed):423
叶任高, 陆再英 .内科学 ( 第6版)[M]. 北京: 人民卫生出版社, 2004,( 第6版):423
[2] Li FL . Electro-acupuncture treatment of 32 cases of irritable bowel syndrome[J]. Zhongguo Zhong Xi Yi Jie He Pi Wei Za Zhi, 2000,8(3):141
李芳莉 . 电针治疗肠激惹综合征32例[J]. 中国中西医结合脾胃杂志, 2000,8(3):141
[3] Yuan X . Electro-acupuncture treatment of one case of irritable bowel syndrome[J]. Shi Yong Zhong Yi Yao Za Zhi, 2005,21(2):108
doi: 10.3969/j.issn.1004-2814.2005.02.051
袁霞 . 电针治疗肠易激综合征1例[J]. 实用中医药杂志, 2005,21(2):108
doi: 10.3969/j.issn.1004-2814.2005.02.051
[4] Chen L, Liu S . High-frequency electro-acupuncture at Zusanli point promotes colonic propulsive motility in rats[J]. Shi Jie Hua Ren Xiao Hua Za Zhi, 2006,14(30):2962-2964
doi: 10.3969/j.issn.1009-3079.2006.30.012
陈兰, 刘诗 . 高频电针刺激足三里促进大鼠结肠推进运动[J]. 世界华人消化杂志, 2006,14(30):2962-2964
doi: 10.3969/j.issn.1009-3079.2006.30.012
[5] Al-Chaer ED, Kawasaki M, Pasricha PJ . A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development[J]. Gastroenterology, 2000,119(5):1276-1285
doi: 10.1053/gast.2000.19576
[6] Cui KM, Li WM, Gao X, Chung K, Chung JM, Wu GC . Electro-acupuncture relieves chronic visceral hyperalgesia in rats[J]. Neurosci Lett, 2005,376(1):20-23
doi: 10.1016/j.neulet.2004.11.018 pmid: 15694267
[7] Sato A, Sato Y, Suzuki A, Uchida S . Neural mechanisms of the reflex inhibition and excitation of gastric motility elicited by acupuncture-like stimulation in anesthetized rats[J]. Neurosci Res, 1993,18(1):53-62
doi: 10.1016/0168-0102(93)90105-Y
[8] Yin X, Ning SB . Advances in understanding the pathogenesis of irritable bowel syndrome[J]. Shi Jie Hua Ren Xiao Hua Za Zhi, 2009,17(32):3318-3323
doi: 10.3969/j.issn.1009-3079.2009.32.008
银新, 宁守斌 . 肠易激综合征发病机制的研究进展[J]. 世界华人消化杂志, 2009,17(32):3318-3323
doi: 10.3969/j.issn.1009-3079.2009.32.008
[9] Drossman DA . The functional gastrointestinal disorders and the Rome Ⅲ process[J]. Gastroenterology, 2006,130(5):1377-1390
doi: 10.1053/j.gastro.2006.03.008 pmid: 16678553
[10] Jia P, Chen G, Chen RX . Effects of electro-acupuncture of Zusanli point(ST36) at different time on derangement of gastric electric rhythm in rabbits[J]. Sichuan Zhong Yi, 2007,25(3):12-14
doi: 10.3969/j.issn.1000-3649.2007.03.007
贾萍, 陈刚, 陈日新 . 电针“足三里”穴对家兔胃电节律紊乱调整的时间效应关系研究[J]. 四川中医, 2007,25(3):12-14
doi: 10.3969/j.issn.1000-3649.2007.03.007
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