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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (2): 129-131.doi: 10.3736/jcim20040216

• Original Experimental Research • Previous Articles     Next Articles

Hemostatic and analgesic effect of Gonghuan Zhixue Tablet on mice

Ling-mei Fu, Zhao-ling You, Lei Lei, Le-xi Wen, Huai-min Chen   

  1. Department of Integrated Traditional Chinese and Western Medicine, Hunan College of Traditional Chinese Medicine, Changsha, Hunan Province 410007, China
  • Online:2004-03-20 Published:2018-10-25

Objective: To explore the hemostatic and analgesic effect of Gonghuan Zhixue Tablet (GHZXT) on mice and to produce experimental evidence for exploiting new drug for endometrorrhagia caused by Cu-intrauterine contraceptive device (Cu-IUD). Methods: Compared with 6-aminocaproic acid and notoginseng, the effects of GHZXT on clotting and bleeding time of mice with capillary method and severed tail were investigated; and compared with aspirin, the analgesic effects of GHZXT on mice were investigated with hot plate and torsive body method. Results: The clotting time of mice was remarkably shortened with a rising of the dosage of GHZXT and the difference between each therapeutic group and distilled water group was remarkable. As compared with distilled water group, the bleeding time of each dosage group of GHZXT was obviously shortened; and each dosage of GHZXT could prolong the time of pain reaction to hot plate and decrease the degree of torsive body of the mice. Conclusion: Pharmacological experiment has proved that GHZXT has evident hemostatic and analgesic function.

Key words: Gonghuan Zhixue Tablet, blood clotting, analgesia, animals, laboratory

CLC Number: 

  • R965

Tab 1

Effects of Gonghuan Zhixue Tablet on clotting and bleeding time of mice (ヌ±S)"

Group Dosage (g/kg) n Clotting time(s) Bleeding time(s)
Distilled water - 10 343.50±21.61 230.60±13.13
6-aminocaproic acid 0.78 10 267.90±24.55* 159.50±13.36*
Notoginseng 1.04 10 248.90±25.73* 150.60±14.10*
Low dosage of GHZXT 0.44 10 273.50±9.82* 145.80±12.76*
Middle dosage of GHZXT 1.31 10 259.40±10.56* 140.90±11.64*▲▲
High dosage of GHZXT 3.94 10 250.50±17.14* 124.40±22.42*△△▲▲

Tab 2

Effects of Gonghuan Zhixue Tablet on pain reaction time of mice to hot plate (ヌ±S)"

Group Dosage (g/kg) n Pain threshold(s)
30 min 60 min 90 min 120 min
Distilled water - 10 3.5±1.6 2.5±1.2 2.2±1.4 1.6±0.9
Low dosage of GHZXT 0.44 10 8.3±3.2*□□■■ 10.1±5.9**□□■■ 6.9±2.3*□□■■ 4.5±1.2*□□■■
Middle dosage of GHZXT 1.31 10 11.4±8.9**△△▲ 14.8±8.9**□□■ 9.3±3.2**□□ 7.6±2.3**□□■
High dosage of GHZXT 3.94 10 16.9±7.6** 18.8±9.4** 13.8±8.9** 9.5±3.4**
Aspirin 0.2 10 18.7±12.4** 20.7±16.4** 15.8±9.6** 10.5±6.3**

Tab 3

Effects of Gonghuan Zhixue Tablet on torsive body reaction of mice (ヌ±S)"

Group Dosage(g/kg) n Torsive body reaction
Cases Frequencies
Distilled water - 10 10 50.2±23.8
Low dosage of GHZXT 0.44 10 8 25.6±11.6**□□■■
Middle dosage of GHZXT 1.31 10 7 22.7±13.5**□□■
High dosage of GHZXT 3.94 10 7 19.8±10.6**
Aspirin 0.20 10 6 16.8±12.8**
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