SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (5): 346-349.doi: 10.3736/jcim20040511

• Original Experimental Research • Previous Articles     Next Articles

Comparative study of kidney-tonifying recipes and spleen-invigorating recipe on regulating pattern of the expression of T lymphocyte apoptosis signaling-related genes in corticosterone-treated rats

Yu Chen(), Zi-yin Shen, Wei-hua Chen   

  1. Institute of Integrated Traditional Chinese and Western Medicine, Fudan University, Shanghai 200040, China
  • Received:2004-05-19 Online:2004-09-20 Published:2018-10-20

RichHTML

1

PDF (PC)

74

Like

Objective: To investigate the regulating pattern of kidney-tonifying recipes and spleen-invigorating recipe on the expression of T lymphocyte apoptosis signaling-related genes in corticosterone-treated rats.Methods: We analyzed the activation-induced T lymphocyte apoptpsis rate through TUNEL-labelled flow-cytometry assay, quantified the mRNA expression level of T cell apoptosis-regulating genes by using the fluoresecence real-time PCR, analyzed the activity of caspase-8 and caspase-3 by colorimetric assay.Results: (1) T lymphocyte apoptosis rate of corticosterone-treated rats was higher than that of the normal control rats; T lymphocyte apoptosis rates of Youguiyin treated rats and Bushen Yishou Capsule treated rats were lower than that of the corticosterone-treated group; T lymphocyte apoptosis rate of Sijunzi Decoction treated rats displayed no significance compared with that of the corticosterone-treated group. (2) Pro-apoptotic genes such as Fas, FasL, TNFR1, and Bax displayed increased expression level in corticosterone-treated group compared with normal control group. Two kidney-tonifying recipes down-regruated the expression level of FasL and TNFR1, at the same time, both recipes up-regulated the expression level of Bcl-2.The spleen-invigorating recipe had up-regulating effect on the expression level of Bcl-2. And all these results had statistical significance. (3) The activities of caspase-8 and caspase-3 were increased in corticosterone-treated rats compared with normal control; both kidney-tonifying recipes had significant effect on decreasing the activities of these two apoptosis executioners.Conclusion: The excessive apoptosis of T cell in corticosterone-treated rats could be effectively modulated with two kidney-tonifying recipes. Down-regulating the expression of FasL and TNFR1 and up-regulating the expression of Bcl-2 as well as decreasing the activities of caspase-8 and caspase-3 may be the unique regulating pattern of tonifying-kidney method with respect to T cell apoptosis related genes in corticosterone-treated rats.

Key words: kidney-reinforcing drugs, apoptosis gene, genes regulator, corticosterone, animals, laboratory

CLC Number: 

  • R289.11

Tab 1

T lymphocyte apoptosis rate in different groups ($\bar{x}$±s )"

Group n T lymphocyte apoptosis
rate （%）
Normal control 10 34.25±6.47
Corticosterone treated 10 45.78±7.22**
Sijunzi Decoction treated 10 36.92±11.82
Youguiyin treated 10 35.90±7.39△△
Bushen Yishou Capsule treated 10 36.20±9.14△

Tab 2

Expression of T lymphocyte pro-apoptotic signaling-related genes in different groups ($\bar{x}$±s )"

Group n Fas FasL TNFR1 Bax
Normal control 8 1.527±0.063△△ 1.553±0.077△△ 1.725±0.127△△ 1.516±0.193△
Corticosterone treated 8 1.422±0.077 1.413±0.050 1.390±0.163 1.332±0.120
Sijunzi Decoction treated 8 1.427±0.062 1.464±0.060 1.519±0.055 1.362±0.125
Youguiyin treated 8 1.486±0.080 1.525±0.067△△ 1.603±0.046△△ 1.489±0.177
Bushen Yishou Capsule treated 8 1.454±0.048 1.512±0.078△△ 1.541±0.070△ 1.369±0.138△

Tab 3

Expression of T lymphocyte anti-apoptotic signaling-related genes in different groups ($\bar{x}$±s )"

Group n TNFR2 Bcl-2 cIAP1 cIAP2
Normal control 8 1.328±0.082△ 1.294±0.134△△ 1.391±0.094△△ 1.421±0.120△△
Corticosterone treated 8 1.525±0.232 1.573±0.223 1.703±0.241 1.722±0.206
Sijunzi Decoction treated 8 1.356±0.087 1.367±0.028△ 1.515±0.193 1.520±0.167△
Youguiyin treated 8 1.375±0.131 1.350±0.163△ 1.445±0.212△ 1.437±0.237△
Bushen Yishou Capsule treated 8 1.348±0.050 1.376±0.046△ 1.478±0.168△ 1.518±0.116△

Tab 4

Activities of caspase-8 and caspase-3 in different groups ($\bar{x}$±s )"

Group n Caspase-8 Caspase-3
Normal control 8 0.125±0.030 0.269±0.094
Corticosterone treated 8 0.190±0.066* 0.491±0.157**
Sijunzi Decoction treated 8 0.158±0.032 0.366±0.079
Youguiyin treated 8 0.132±0.031△ 0.299±0.126△
Bushen Yishou Capsule treated 8 0.134±0.025△ 0.354±0.027△
[1] 蔡定芳, 沈自尹, 张玲娟 , 等. 右归饮对皮质酮大鼠细胞免疫及细胞因子的影响[J]. 中国免疫学杂志, 1995,11(4):248-251
[2] Severson CD, Burg CL, Lafrenz DE , et al. An alternative method of panning for rat B lymphocytes[J]. Immunol Lett, 1987,15(4):291-295
doi: 10.1016/0165-2478(87)90130-1 pmid: 3500910
[3] Kischkel FC, Hellbardt S, B ehrmann I , et al. Cytotoxity-dependent APO-1(Fas/CD95)-assiociated proteins form a death-inducing signaling complex(DISC) with the receptor[J]. EMBO J, 1995,14(22):5579-5588
doi: 10.1002/embj.1995.14.issue-22
[4] Wiegmann K, Schutze S, Kampen E , et al. Human 55-kDa receptor for tumor necrosis factor coupled to signal transduction cascades[J]. J Biol Chem, 1992,267(25):17997-18001
[5] Oltvai ZN, Milliman CL, Korsmeyer SJ . Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death[J]. Cell, 1993,74(4):609-619
doi: 10.1016/0092-8674(93)90509-O
[6] 沈自尹 . 中医药在调节基因平衡上的优势[J]. 中西医结合学报, 2003,1(1):3-4
doi: 10.11656/j.issn.1672-1519.2014.04.01
Shen ZY . Superiority of traditional Chinese medicine on gene regulation[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(1):3-4
[7] 沈自尹 . 肾阳虚证的定位研究[J]. 中国中西医结合杂志, 1997,17(1):50-52
[8] 郭为民, 沈自尹, 陈瑜 , 等. 补肾、活血类复方对老年大鼠T细胞凋亡相关基因表达调控模式的比较研究[J]. 中国中西医结合杂志, 2002,22(3):203-206
doi: 10.7661/CJIM.2002.3.203
[1] Cai Lin-lin, Li Hao, Liu Jian-gang, Liu Long-tao, Guan Jie, Liu Ming-fang, Hu Jia, Wei Yun. Effects of early intervention with Huannao Yicong formula effective components on behavior and cholinergic system of β-amyloid precursor protein transgenic mice. Journal of Chinese Integrative Medicine, 2011, 9(3): 292-298.
[2] Qin Bian, Jian-hua Huang, Zhu Yang, You Ning, Yong-jian Zhao, Yong-jun Wang, Zi-yin Shen. Effects of active ingredients in three kidney-tonifying Chinese herbal drugs on gene expression profile of bone marrow stromal cells from a rat model of corticosterone-induced osteoporosis. Journal of Chinese Integrative Medicine, 2011, 9(2): 179-185.
[3] Su-qin Zhang, Zi-yin Shen, Guo-rang Hu, Run-hong Liu, Xin-ming Zhang. Effects of Chinese herbal formula Biminne in regulating endocrine-immune function in mice with airway allergy. Journal of Chinese Integrative Medicine, 2009, 7(4): 349-353.
[4] You-xue Chen, Guo-ping Li, Hong-en Yao, Su-fan Li, Li-gong Duan. Bushen Jianpi Huoxue Recipe in prevention and treatment of tibial stress injuries in rabbits. Journal of Chinese Integrative Medicine, 2008, 6(7): 738-743.
[5] Yong-jun Wang, Qi Shi, Jian-chun Jiang, Qian-qian Liang, Qin Bian, Chen-guang Li, Quan Zhou, Xue-jun Cui, Min Huang, Qing-gao Liu, Sheng Lu, Chong-jian Zhou. Establishment of a rat model of cervical syndrome with qi deficiency, blood stasis and kidney deficiency. Journal of Chinese Integrative Medicine, 2008, 6(11): 1152-1158.
[6] Qi Li​, Zhong-ze Fan​, Xian-qian Li​, Xiao-hua Liu​, Jue Sun​, Wei Gu​, Paul Heng​, Hong Gao​. Effects of interventional therapy with norcantharidin microsphere on hepatoma in rats and its mechanism. Journal of Chinese Integrative Medicine, 2006, 4(4): 378-383.
[7] Yue-lai Chen, Jue Cen, Wen-guang Hou, Zhi-qiang Gao, Xian-min Yu, Xue-mei Ma. Effects of electroacupuncture treatment on nitrergic neurotransmitter in bladder neck and detrusor of rats with unstable bladder. Journal of Chinese Integrative Medicine, 2006, 4(1): 73-75.
[8] Jun-xiang Li, Zhi-bin Wang, Ling-qun Zhu, Fu-ling Niu, Wei Cui. Apoptosis-promoting effect of Panax notoginseng extracts on MNNG-transformed GES-1 cells. Journal of Chinese Integrative Medicine, 2005, 3(2): 123-127.
[9] Jian-hong Zhou, Dong-feng Chen, Shao-hui Du, Hui Li, Yi-wei Li, Ru-dong Deng, Sai-xia Zhang. Effect of Niupo Zhibao Pellet on expression of neuronal nitric oxide synthase in brain of endotoxin-induced shock rats. Journal of Chinese Integrative Medicine, 2005, 3(2): 115-118.
[10] Bin Zhang, Mo-bin Wan, Ling-tai Wang. Therapeutic effect of Bushen Rougan Recipe on hepatic fibrosis in rats. Journal of Chinese Integrative Medicine, 2005, 3(2): 132-135.
[11] Li Liu, Hong-quan Zhang​. Effects of Danshen Decoction on experimental gastric ulcer in rats and mice. Journal of Chinese Integrative Medicine, 2005, 3(1): 35-38.
[12] Hong-li Xue, Wen-jian Wang, Jian-qiu Chen. Effects of Shenmai Huoxue Decoction on early diabetic peripheral neuropathy in rats. Journal of Chinese Integrative Medicine, 2005, 3(1): 31-34.
[13] Le-hong Jin, Chuan-fei Liu, Yu Zeng. Protective effects of puerarin on radiation injury of experimental rats. Journal of Chinese Integrative Medicine, 2005, 3(1): 43-45.
[14] Zhi-bin Wang, Jun-xiang Li, Ling-qun Zhu, Fu-ling Niu, Wei Cui. Inhibiting effects of Panax notoginseng extracts on proliferation of GES-1 cells and MNNG-transformed GES-1 cells. Journal of Chinese Integrative Medicine, 2004, 2(6): 445-449.
[15] Mu-rong Liu, Ting Han, Yao Chen, Lu-ping Qin, Han-chen Zheng, Yao-cheng Rui. Effect of madecassoside on depression behavior of mice and activities of MAO in different brain regions of rats. Journal of Chinese Integrative Medicine, 2004, 2(6): 440-444.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3