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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (5): 346-349.doi: 10.3736/jcim20040511

• Original Experimental Research • Previous Articles     Next Articles

Comparative study of kidney-tonifying recipes and spleen-invigorating recipe on regulating pattern of the expression of T lymphocyte apoptosis signaling-related genes in corticosterone-treated rats

Yu Chen(), Zi-yin Shen, Wei-hua Chen   

  1. Institute of Integrated Traditional Chinese and Western Medicine, Fudan University, Shanghai 200040, China
  • Received:2004-05-19 Online:2004-09-20 Published:2018-10-20

Objective: To investigate the regulating pattern of kidney-tonifying recipes and spleen-invigorating recipe on the expression of T lymphocyte apoptosis signaling-related genes in corticosterone-treated rats.Methods: We analyzed the activation-induced T lymphocyte apoptpsis rate through TUNEL-labelled flow-cytometry assay, quantified the mRNA expression level of T cell apoptosis-regulating genes by using the fluoresecence real-time PCR, analyzed the activity of caspase-8 and caspase-3 by colorimetric assay.Results: (1) T lymphocyte apoptosis rate of corticosterone-treated rats was higher than that of the normal control rats; T lymphocyte apoptosis rates of Youguiyin treated rats and Bushen Yishou Capsule treated rats were lower than that of the corticosterone-treated group; T lymphocyte apoptosis rate of Sijunzi Decoction treated rats displayed no significance compared with that of the corticosterone-treated group. (2) Pro-apoptotic genes such as Fas, FasL, TNFR1, and Bax displayed increased expression level in corticosterone-treated group compared with normal control group. Two kidney-tonifying recipes down-regruated the expression level of FasL and TNFR1, at the same time, both recipes up-regulated the expression level of Bcl-2.The spleen-invigorating recipe had up-regulating effect on the expression level of Bcl-2. And all these results had statistical significance. (3) The activities of caspase-8 and caspase-3 were increased in corticosterone-treated rats compared with normal control; both kidney-tonifying recipes had significant effect on decreasing the activities of these two apoptosis executioners.Conclusion: The excessive apoptosis of T cell in corticosterone-treated rats could be effectively modulated with two kidney-tonifying recipes. Down-regulating the expression of FasL and TNFR1 and up-regulating the expression of Bcl-2 as well as decreasing the activities of caspase-8 and caspase-3 may be the unique regulating pattern of tonifying-kidney method with respect to T cell apoptosis related genes in corticosterone-treated rats.

Key words: kidney-reinforcing drugs, apoptosis gene, genes regulator, corticosterone, animals, laboratory

CLC Number: 

  • R289.11

Tab 1

T lymphocyte apoptosis rate in different groups ($\bar{x}$±s )"

Group n T lymphocyte apoptosis
rate (%)
Normal control 10 34.25±6.47
Corticosterone treated 10 45.78±7.22**
Sijunzi Decoction treated 10 36.92±11.82
Youguiyin treated 10 35.90±7.39△△
Bushen Yishou Capsule treated 10 36.20±9.14

Tab 2

Expression of T lymphocyte pro-apoptotic signaling-related genes in different groups ($\bar{x}$±s )"

Group n Fas FasL TNFR1 Bax
Normal control 8 1.527±0.063△△ 1.553±0.077△△ 1.725±0.127△△ 1.516±0.193
Corticosterone treated 8 1.422±0.077 1.413±0.050 1.390±0.163 1.332±0.120
Sijunzi Decoction treated 8 1.427±0.062 1.464±0.060 1.519±0.055 1.362±0.125
Youguiyin treated 8 1.486±0.080 1.525±0.067△△ 1.603±0.046△△ 1.489±0.177
Bushen Yishou Capsule treated 8 1.454±0.048 1.512±0.078△△ 1.541±0.070 1.369±0.138

Tab 3

Expression of T lymphocyte anti-apoptotic signaling-related genes in different groups ($\bar{x}$±s )"

Group n TNFR2 Bcl-2 cIAP1 cIAP2
Normal control 8 1.328±0.082 1.294±0.134△△ 1.391±0.094△△ 1.421±0.120△△
Corticosterone treated 8 1.525±0.232 1.573±0.223 1.703±0.241 1.722±0.206
Sijunzi Decoction treated 8 1.356±0.087 1.367±0.028 1.515±0.193 1.520±0.167
Youguiyin treated 8 1.375±0.131 1.350±0.163 1.445±0.212 1.437±0.237
Bushen Yishou Capsule treated 8 1.348±0.050 1.376±0.046 1.478±0.168 1.518±0.116

Tab 4

Activities of caspase-8 and caspase-3 in different groups ($\bar{x}$±s )"

Group n Caspase-8 Caspase-3
Normal control 8 0.125±0.030 0.269±0.094
Corticosterone treated 8 0.190±0.066* 0.491±0.157**
Sijunzi Decoction treated 8 0.158±0.032 0.366±0.079
Youguiyin treated 8 0.132±0.031 0.299±0.126
Bushen Yishou Capsule treated 8 0.134±0.025 0.354±0.027
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