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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (6): 620-623.doi: 10.3736/jcim20060616

• Original Experimental Research • Previous Articles     Next Articles

Effects of herbs for replenishing yin to tonify kidney on testicular ultrastructural changes in rats with glucocorticoid-induced kidney deficiency

Jian Ying1,Hai-yong Chen2,Fu-song Xu3   

  1. 1. Department of Rehabilitation, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Hong Kong Baptist University, Hong Kong, China
    3. Department of Andrology, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
  • Online:2006-11-30 Published:2006-11-15

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Objective

To investigate the effects of herbs for replenishing yin to tonify kidney on testicular ultrastructural changes in rats with glucocorticoid-induced kidney deficiency.

Methods

Thirty rats were randomized into three groups: normal control group, untreated group and Erdi Biejia Decoction (EDBJD)-treated group. Kidney deficiency rat model was induced by glucocorticoid. Testicular structure and ultrastructure were observed under the light microscope and the transmission electron microscope.

Results

The light microscope revealed the decreased thickness of seminiferous epithelium layer, reduced number of spermatogenic cells, interstitial tissue edema, and a qualitative reduction in the number of interstitial cells and hematoxylin-eosin stained cytoplasm in the untreated group. The transmission electron microscope revealed loose structure of seminiferous epithelium, degenerative mitochondria, and increased amounts of lysosome, presence of numerous cytoplasmic vacuolation and chromatin in interstitial cell cytoplasm in the untreated group. The aggregates of heterochromatin around the nuclear membrane and the shrinkage of nucleus were also observed. Compared to the untreated group, these pathological changes were inconspicuous in EDBJD-treated group.

Conclusion

EDBJD as replenishing yin to tonify kidney herbs can suppress glucocorticoid induced pathological changes in the testes of rats.

Key words: Reinforcing kidney, Nourishing yin, Testis, Ultrastructure, Sex hormones, Rats

CLC Number: 

  • R69

Figure 1

Morphological changes of testicular tissue of a rat in untreated group by an optical microscope.HE staining. A: ×100; B: ×400; C: ×400; D: ×400."

Figure 2

Morphological changes of testicular tissue of a rat in EDBJD-treated group by an optical microscope. HE staining. A: ×100; B: ×400; C: ×400; D: ×400."

Figure 3

Ultrastructure of testicular tissue of a rat in untreated group by an electron microscope.A: ×6 000; B: ×60 000; C: ×25 000; D: ×30 000; E: ×12 000; F: ×5 000; G: ×8 000."

Figure 4

Ultrastructure of testicular tissue of a rat in EDBJD-treated group by an electron microscope. A: ×8 000; B: ×15 000; C: ×8 000."

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