SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 686-691.doi: 10.3736/jcim20070617

Special Issue: Acupuncture & Moxibustion

• Original Experimental Research • Previous Articles     Next Articles

Effect of scalp acupuncture on inflammatory response in rats with acute cerebral ischemia-reperfusion injury

Hong-xing Zhang, Ling-guang Liu, Li Zhou, Hao Huang, Xuan Li, Min Yang   

  1. Department of Acupuncture & Moxibustion , Wuhan Chinese and Western Medicine Hospital , Wuhan , Hubei Province 430022 ,China
  • Online:2007-11-20 Published:2007-11-15

RichHTML

1

PDF (PC)

80

Cited

27

Like

Objective To explore the mechanism of scalp acupuncture (SA) in treating cerebral ischemia.Methods: Sixty SD rats with middle cerebral artery occlusion (MCAO) were randomized into untreated group and SA-treated group, with another group of 10 SD rats without artery occlusion as sham-operated control. Neurological severity score (NSS), hemetoxylin and eosin (HE) staining and enzyme-linked immunosorbent assay were applied to observing the changes of neurofunctional defect, inflammatory infiltration in cerebral tissue and content of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-10 at 24, 48 and 72 h after ischemia-reperfusion.Results: There existed significant difference in NSS between SA-treated group and untreated group (P<0.01), especially at 72 h after ischemia-reperfusion. The HE staining results of ischemic cerebral tissue showed an apparent reduction of inflamed lesions in SA-treated group as compared with the untreated group (P<0.01), especially at 72 h after ischemia-reperfusion. The content of TNF-α and IL-1β at each phase point after ischemia-reperfusion in SA-treated was decreased as compared with that in the untreated group, and there were differences between SA-treated group and untreated group at 72 h after ischemia-reperfusion (P<0.01). An apparent increase was observed in IL-10 between SA-treated group and untreated group at each phase point after ischemia-reperfusion, and there were significant differences between SA-treated group and untreated group at 48 and 72 h after ischemia-reperfusion (P<0.05, P<0.01).Conclusion: Scalp acupuncture can improve neurofunctional rehabilitation, suppress leukocyte infiltration, decrease the content of TNF-α and IL-1β within a certain range and enhance IL-10 expression so as to suppress cytokines-mediated inflammatory reaction and attenuate cerebral ischemia-reperfusion injury.

Key words: scalp acupuncture, brain ischemia, reperfusion, inflammation, rats

CLC Number: 

  • R743.31

Table 1

Comparison of neurological severity score among different groups ($\bar{x}±S$)"

Group n NSS
Sham-operated 10 0
Untreated
24 h after ischemia-reperfusion 10 2.30±0.67**
48 h after ischemia-reperfusion 10 2.10±0.57**
72 h after ischemia-reperfusion 10 2.00±0.47**
SA-treated
24 h after ischemia-reperfusion 10 1.90±0.57**△△
48 h after ischemia-reperfusion 10 1.70±0.48**△△
72 h after ischemia-reperfusion 10 1.30±0.48**△△

Table 2

Number of HE stained inflammatory cells in different groups ($\bar{x}±S$)"

Group n Inflammatory cell number
Sham-operated 10 1.10±0.56
Untreated
24 h after ischemia-reperfusion 10 12.50±1.27**
48 h after ischemia-reperfusion 10 18.10±1.37**
72 h after ischemia-reperfusion 10 11.10±1.20**
SA-treated
24 h after ischemia-reperfusion 10 12.30±1.25**
48 h after ischemia-reperfusion 10 12.10±1.20**△△
72 h after ischemia-reperfusion 10 7.00±1.41**△△

Figure 1

Brain tissue in different groups (HE staining, ×400)A: Sham-operated group; B: Untreated group at 24 h after ischemia-reperfusion; C: Untreated group at 48 h after ischemia-reperfusion; D: Untreated group at 72 h after ischemia-reperfusion; E: SA-treated group at 24 h after ischemia-reperfusion; F: SA-treated group at 48 h after ischemia-reperfusion; G: SA-treated group at 72 h after ischemia-reperfusion."

Table 3

Plasma content of TNF-α, IL-1β and IL-10 in rats with brain ischemia-reperfusion in different groups (ng/L, $\bar{x}±S$)"

Group n TNF-α IL-1β IL-10
Sham-operated 10 4.94±0.97 56.34±6.31 64.02±9.80
Untreated
24 h after ischemia-reperfusion 10 7.69±1.44** 66.29±9.78** 138.86±14.66**
48 h after ischemia-reperfusion 10 9.13±1.83* 74.04±7.80** 134.27±13.93**
72 h after ischemia-reperfusion 10 11.37±1.31** 64.12±9.01* 166.55±11.04**
SA-treated
24 h after ischemia-reperfusion 10 6.51±1.36** 65.65±6.31* 143.36±10.76**
48 h after ischemia-reperfusion 10 8.24±1.62** 73.44±6.87** 151.67±15.50**△
72 h after ischemia-reperfusion 10 7.08±1.63**△△ 52.57±7.79△△ 196.26±26.02**△△

Table 4

Brain content of TNF-α, IL-1β and IL-10 in rats with brain ischemia-reperfusion in different groups (ng/L, $\bar{x}±S$)"

Group n TNF-α IL-1β IL-10
Sham-operated 10 75.41±10.33 99.31±7.37 95.16±15.20
Untreated
24 h after ischemia-reperfusion 10 104.79±9.71** 123.09±5.80** 169.74±23.15**
48 h after ischemia-reperfusion 10 125.68±9.11** 139.06±5.93** 234.54±26.40**
72 h after ischemia-reperfusion 10 145.44±17.96** 131.72±5.34** 204.76±24.59**
SA-treated
24 h after ischemia-reperfusion 10 95.31±9.76** 118.90±7.70** 179.80±24.84**
48 h after ischemia-reperfusion 10 117.67±10.69** 135.67±4.02** 276.43±30.35**△△
72 h after ischemia-reperfusion 10 121.74±11.69**△△ 102.69±7.96△△ 235.96±23.47**△△
[1] 王萍 . 头针治疗中风200例临床观察[J]. 针灸临床杂志, 2006,22(12):54-55
Wang P . Clinical observations on 200 patients of apoplexy by head needling[J]. Zhen Jiu Lin Chuang Za Zhi, 2006,22(12):54-55
[2] 张红星, 张唐法 . 头针治疗中风及对血浆内皮素含量的影响[J]. 中国针灸, 2002,22(12):831-832
Zhang HX, Zhang TF . Scalp Acupuncture for Treatment of Apoplexy and Effect on Plasma ET Content[J]. Zhong Guo Zhen Jiu, 2002,22(12):831-832
[3] 张家良, 张小燕 . 头针对急性脑卒中的临床疗效[J]. 中国康复, 2007,22(2):121
Zhang JL, Zhang XY . Clinical Effects of Scalp Acupuncture on Acute stroke[J]. Zhong Guo Kang Fu, 2007,22(2):121
[4] Longa EZ, Weinstein PR, Carlson S , et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989,20(1):84-91
[5] 华兴邦 . 大鼠穴位图谱的研制[J]. 实验动物与动物实验, 1991,( 1):1-5
Hua XB . Design of Rat Acupoint[J]. AtlasShi Yan Dong Wu Yu Dong Wu Shi Yan, 1991,( 1):1-5
[6] Lu A, Tang Y, Ran R , et al. Genomics of the Periinfarction Cortex After Focal Cerebral Ischemia[J]. J Cereb Blood Flow Metab, 2003,23(7):786-810
doi: 10.1097/01.WCB.0000062340.80057.06 pmid: 12843783
[7] Mergenthaler P, Dirnagl U, Meisel A . Pathophysiology of Stroke: Lessons from Animal Models[J]. Metab Brain Dis, 2007,19(3-4):151-167
[8] 罗梅宏, 周永明 . 中药抗组织损伤与白细胞黏附[J]. 中西医结合学报, 2005,3(4):322-325
Luo MH, Zhou YM . Leukocyte adhesion and prevention of tissue injury with traditional Chinese medicine[J]. Zhong Xi Yi Jie He Xue Bao, 2005,3(4):322-325
[9] 梁健芬, 董少龙, 窦维华 , 等. 水蛭注射液对大鼠脑缺血再灌注后TNF-α、IL—1β和ICAM-1蛋白表达的影响[J]. 陕西医学杂志, 2006,35(12):1605-1607
Liang JF, Dong SL, Dou WH, Hu YQ . Effects of ShuiZhi Injection on TNF-α, IL-lβ and ICAM-1 Expression in Rats with Cerebral Ischemic Reperfusion Injury[J]. Shan Xi Yi Xue Za Zhi, 2006,35(12):1605-1607
[10] Feuerstein G, Wang X, Barone F . Cytokins in brain ischemia: the role of TNF-alpha. Cell Mol Neurobiol[J]. Shan Xi Yi Xue Za Zhi, 1998,18(6):695-701
[11] Fabian RH, Perez-Polo JR, Kent TA . Electrochemical monitoring of superoxide anion production and cerebral blood flow: effect of interleukin-1 beta pretreatment in a model of focal ischemia and reperfusion[J]. J Neurosci Res, 2000,60(6):795-803
[12] 曲东锋, 倪长江, 李宏建 . 卒中后炎症反应——有益还是有害?[J]. 国外医学脑血管疾病分册, 2002,10(5):343-344
Qu DF, Ni CJ, Li HJ . Inflammation after stroke: beneficial or malefic?[J]. Guo Wai Yi Xue Nao Xue Guan Ji Bing Fen Ce, 2002,10(5):343-344
[13] 陈燕启, 刘德红, 姜宏志 , 等. 葛根素对脑缺血-再灌流时大鼠肿瘤坏死因子α和白细胞介素-1β表达的影响[J]. 中华急诊医学杂志, 2005,14(2):119-121
Chen YQ, Liu DH, Jiang HZ, Yang GT . Effects of puerarin on TNF-α mRNA and IL -1β mRNA in Rats after global cerebral ischemia-reperfusion[J]. Zhong Hua Ji Zhen Yi Xue Za Zhi, 2005,14(2):119-121
[14] Wang Q, Tang XN, Yenari MA . The inflammatory response in stroke[J]. J Neuroimmunol, 2007,184(1-2):53-68
doi: 10.1016/j.jneuroim.2006.11.014 pmid: 17188755
[15] van Exel E, Gussekloo J, de Craen AJ, at al . Inflammation and stroke: the Leiden 85-Plus Study[J]. Stroke, 2002,33(4):1135-1138
doi: 10.1161/01.STR.0000014206.05597.9E pmid: 11935072
[16] Murakami Y, Saito K, Hara A , et al. Increases in tumor necrosis factor-alpha following transient global cerebral ischemia do not contributes to neuron death in mouse hippocampus[J]. J Neurochem, 2005,93(6):1616-1622
[17] Offner H, Subramanian S, Parker SM , et al. Experimental stroke induces massive, rapid activation of the peripheral immune system[J]. J Cereb Blood Flow Metab, 2006,26(5):654-665
[18] Skifter DA, Allegrini PR, Wiessner C , et al. Similar Time-Course of Interleukin-1Beta Production and Extracellular-Signal-Regulated kinase(ERK) Activation in Permanent Focal brain Ischemic Injury[J]. Metab Brain Dis, 2002,17(3):131-138
doi: 10.1023/A:1019917803470
[19] Haqqani AS, Nesic M, Preston E , et al. Characterization of vascular protein expression patterns in cerebral ischemia/reperfusion using laser capture microdissection and ICAT-nanoLC-MS/MS[J]. FASEB J, 2005,19(13):1809-1821
[20] 王文华, 卞杰勇, 王中 , 等. 大鼠永久性脑缺血后白细胞介素IL-1β表达的研究[J]. 苏州大学学报(医学版), 2005,25(6):986-988
Wang WH, Bian JY, Wang Z , et al. Interleukin-1β mRNA Expression after Permanent Cerebral Ischemia in Rats[J]. Suzhou Da Xue Xue Bao(Yi Xue Ban), 2005,25(6):986-988
[21] Li HL, Kostulas N, Huang YM , et al. IL-17 and IFN-gamma mRNA expression is increased in the brain and systemically after permanent middle cerebral artery occlusion in the rat[J]. J Neuroimmunol, 2006,116(1):5-14
[22] Zhai QH, Futrell N, Chen FJ . Gene expression of IL-10 in relationship to TNF-alpha, IL-1beta and IL-2 in the rat brain following middle cerebral artery occlusion[J]. J Neurol Sci, 1997,152(2):119-124
[23] 张红星, 周利, 张唐法 . 头针对大鼠急性局灶性脑缺血再灌注损伤的影响[J]. 浙江中西医结合杂志, 2006,16(5):268-269
Zhang HX, Zhou L, Zhang TF . Effect of Scalp Acupuncture on Cerebral Blood Flow of the Rat Model with Acute Regional Cerebral Ischemia and Reperfusion Injury[J]. Zhejiang Zhong Xi Yi Jie He Za Zhi, 2006,16(5):268-269
[24] 周利, 张红星, 张唐法 . 头针治疗卒中易感型自发性高血压大鼠的实验研究[J]. 上海针灸杂志, 2006,25(6):42-44
Zhou L, Zhang HX . Study of Scalp Acupuncture on Spontaneous Hypertensive Rats-Stroke Prone after Stroke[J]. Shanghai Zhen Jiu Za Zhi, 2006,25(6):42-44
[25] 吴广伟 . 针刺治疗缺血性脑中风30例疗效观察[J]. 国际医药卫生导报, 2006,12(22):79-80
Wu GW . Observation of Acupuncture on 30 Patients with Ischemic Stroke.[J]. Guo Ji Yi Yao Wei Sheng Dao Bao, 2006,12(22):79-80
[1] Keat Lam Ho, Phaik Har Yong, Chee Woon Wang, Umah Rani Kuppusamy, Chek Tung Ngo, Festo Massawe, Zhi Xiang Ng. Peperomia pellucida (L.) Kunth and eye diseases: A review on phytochemistry, pharmacology and toxicology. Journal of Integrative Medicine, 2022, 20(4): 292-304.
[2] Jia-yi Zhao, Jin Pu, Jian Fan, Xin-yu Feng, Jian-wen Xu, Rong Zhang, Yan Shang. Tanshinone IIA prevents acute lung injury by regulating macrophage polarization. Journal of Integrative Medicine, 2022, 20(3): 274-280.
[3] Peter Bablis, Henry Pollard, Anthony L. Rosner. Stress reduction via neuro-emotional technique to achieve the simultaneous resolution of chronic low back pain with multiple inflammatory and biobehavioural indicators: A randomized, double-blinded, placebo-controlled trial. Journal of Integrative Medicine, 2022, 20(2): 135-144.
[4] Victor Abou Nehmi Filho, Gilson Masahiro Murata, Ruan Carlos Macêdo de Moraes, Gabriely Cristina Alves Lima, Danielle Araujo de Miranda, Katrin Radloff, Raquel Galvão Figuerêdo Costa, Joyce de Cassia Rosa de Jesus, Jéssica Alves De Freitas, Nayara Izabel Viana, Ruan Pimenta, Katia Ramos Moreira Leite, José Pinhata Otoch, Ana Flávia Marçal Pessoa. A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice. Journal of Integrative Medicine, 2021, 19(5): 439-450.
[5] Na Zhi, Qian Mo, Shuo Yang, Yuan-xing Qin, Hao Chen, Zeng-guang Wu, Cai-hong Lan, Jun Zhang, Yin-long Li. Treatment of pulmonary fibrosis in one convalescent patient with corona virus disease 2019 by oral traditional Chinese medicine decoction: A case report. Journal of Integrative Medicine, 2021, 19(2): 185-190.
[6] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[7] Swatantra Kumar, Vimal K Maurya, Debadatta Nayak, Anil Khurana, Raj K Manchanda, Srinivasulu Gadugu, Madan LB Bhatt, Shailendra K Saxena. Calcarea carbonica treatment rescues lipopolysaccharide-induced inflammatory response in human mononuclear cells via downregulation of inducible cyclooxygenase pathway. Journal of Integrative Medicine, 2020, 18(5): 441-449.
[8] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[9] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[10] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[11] Uday P. Pratap, Hannah P. Priyanka, Karthik R. Ramanathan, Vishak Raman, Lalgi Hima, Srinivasan Thyagarajan. Noni (Morinda citrifolia L.) fruit juice delays immunosenescence in the lymphocytes in lymph nodes of old F344 rats. Journal of Integrative Medicine, 2018, 16(3): 199-207.
[12] Byung Hyuk Han, Yun Jung Lee, Jung Joo Yoon, Eun Sik Choi, Seung Namgung, Xian Jun Jin, Da Hye Jeong, Dae Gill Kang, Ho Sub Lee. Hwangryunhaedoktang exerts anti-inflammation on LPS-induced NO production by suppressing MAPK and NF-κB activation in RAW264.7 macrophages. Journal of Integrative Medicine, 2017, 15(4): 326-336.
[13] Xiao-ping Huang, Huang Ding, Jin-dong Lu, Ying-hong Tang, Bing-xiang Deng, Chang-qing Deng . Autophagy in cerebral ischemia and the effects of traditional Chinese medicine. Journal of Integrative Medicine, 2015, 13(5): 289-296.
[14] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[15] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3