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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (5): 498-501.doi: 10.3736/jcim20080513

• Original Experimental Research • Previous Articles     Next Articles

Change of aquaporin-1 in rat models of kidney-yang-deficiency syndrome

Yi Li, Li-qun He()   

  1. Department of Nephrology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China
  • Received:2008-03-14 Online:2008-05-20 Published:2008-05-15
  • Contact: HE Li-qun

Objective: To explore the material foundation of kidney-yang-deficiency syndrome.
Methods: Two kinds of rat models of deficiency of kidney-yang were induced by adenine intragastric administration (model Ⅰ) and hydrocortisone intramuscular injection (model Ⅱ). One hundred rats were randomly divided into normal control group, model Ⅰ group, low-dose model Ⅱ group, medium-dose model Ⅱ group and high-dose model Ⅱ group. After model establishment, contents of serum creatinine (SCr), blood urea nitrogen (BUN), and urine creatinine (UCr) were detected by automatic biochemistry analyzer; freezing point method was used for 24-hour urinary osmotic pressure (Uosm) testing and kaolinite method was used to detect the content of urinary 17-hydroxycorticosteroid (17-OHCS). Expression of aquaporin-1 in renal tissue was observed by using real time reverse transcription polymerase chain reaction.
Results: The rats of model groups had the characteristics of kidney-yang deficiency syndrome. The contents of SCr and BUN were significantly higher in model Ⅰgroup than in normal control group and three model Ⅱ groups (P<0.05), and UCr and Uosm were significantly lower (P<0.05). The level of urinary 17-OHCS and expression of aquaporin-1 in renal tissue were decreased in the model Ⅰ group (P<0.05) as compared with the normal control group. Compared with the normal control group, the content of SCr was increased and urinary 17-OHCS was decreased in the medium-dose model Ⅱ group, but the expression of aquaporin-1 was increased in three model Ⅱ groups with a dose-dependent manner.
Conclusion: Aquaporin-1 may be one of the material foundations of kidney-yang-deficiency syndrome.

Key words: deficiency of kidney-yang, aquaporin-1, rats

CLC Number: 

  • R223.1+1

Table 1

Contents of SCr and BUN in rats in different groups ($\bar{x}$±s)"

Group n SCr (μmol/L) BUN (mmol/L)
Normal control 20 29.25±2.77 6.17±0.31
Model Ⅰ 20 160.01±2.04* 40.10±0.44*
Low-dose model Ⅱ 20 34.06±1.04 8.21±0.71
Medium-dose model Ⅱ 20 34.80±4.63* 7.32±0.46
High-dose model Ⅱ 20 32.47±2.03 7.49±0.84

Table 2

Contents of UCr and urinary 17-OHCS, Uosm of 24-hour urine in rats in different groups ($\bar{x}$±s)"

Group n UCr (μmol/L) Uosm [mOsm/(kg·H2O)] Urinary 17-OHCS (μmol/L)
Normal control 20 3 875.75±629.55 2 111.05±540.50 9.34±2.50
Model Ⅰ 20 46.23±59.61* 534.03±56.87* 5.07±2.04*
Low-dose model Ⅱ 20 3 770.80±2 721.40 1 936.30±196.81 8.77±6.00
Medium-dose model Ⅱ 20 3 383.45±2 410.63 1 940.25±473.45 6.22±4.78*
High-dose model Ⅱ 20 2 632.50±2 439.05 1 991.60±509.34 7.61±4.80

Table 3

Expression of aquaporin-1 in renal tissue of rats in different groups ($\bar{x}$±s)"

Group n AQP-1
Normal control 5 0.09±0.03
Model Ⅰ 5 0.05±0.04*
Low-dose model Ⅱ 5 0.13±0.09*
Medium-dose model Ⅱ 5 0.35±0.43*
High-dose model Ⅱ 5 2.48±2.05*
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