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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (6): 595-599.doi: 10.3736/jcim20080610

• Original Experimental Research • Previous Articles     Next Articles

Influence of Feixian Formula on serum transforming growth factor-β1 and platelet-derived growth factor in rats with bleomycin-induced pulmonary fibrosis

Wei Zhang1, Liang-duo Jiang1(), Xiao-mei Zhang2, Jian-jun Wu3, Yuan Deng4, Xiang-feng Lu5   

  1. 1. Department of Respiratory Diseases, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
    2. Department of Respiratory Diseases, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
    3. Department of Traditional Chinese Medicine, Beijing Royal Integrative Medicine Hospital, Beijing 102209, China
    4. Journal of Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China
    5. Department of Pathology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
  • Received:2007-08-02 Online:2008-06-20 Published:2008-06-15
  • Contact: JIANG Liang-duo

Objective: To observe the effects of Feixian Formula, a compound traditional Chinese herbal medicine for treating pulmonary fibrosis, on bleomycin-induced pulmonary fibrosis in rats, and its influence on serum transforming growth factor-β1 (TGF-β1) and platelet-derived growth factor (PDGF).
Methods: Seventy-two male Wistar rats were infused with bleomycin (1 mg/kg) through tracheal intubation to induce pulmonary fibrosis, and they were randomly divided into untreated group (n=24), prednisone-treated group (n=24) and Feixian Formula-treated group (n=24). Fifteen male Wistar rats of the sham-operated group were infused with equivalent normal saline. Twenty-four hours after operation, prednisone (5 mg/kg) and Feixian Formula (1.25 g/kg) were given to the prednisone-treated group and Feixian Formula-treated group respectively by intragastric administration once a day. Equivalent saline was administered to rats of the untreated group and sham-operated group. On the 14th, 28th and 45th day, 5 rats in the sham-operated group and 8 rats in each of the other three groups were dissected to observe pathologic changes of the lung tissues, and the levels of serum TGF-β1 and PDGF were determined by enzyme-linked immunosorbent assay.
Results: At the 45th day, the degree of pulmonary interstitial fibrosis was lesser in rats of the Feixian Formula-treated group as compared with those of the untreated group and prednisone-treated group. The levels of serum TGF-β1 and PDGF were increased, and were significantly higher than those of the sham-operated group, especially on the 45th day (P<0.05). Changes of TGF-β1 level in the prednisone-treated group and the Feixian Formula-treated group were similar to untreated group (P>0.05), and there was no significant difference between the prednisone-treated group and the Feixian Formula-treated group (P>0.05). PDGF in the Feixian Formula-treated group reached the highest level on the 14th day, significantly higher than those of the other three groups (P<0.01). Then it decreased, and was close to that of the sham-operated group on the 45th day (P=0.792). The levels of PDGF in untreated group and prednisone-treated group were increased depending on time, and were obviously higher than that of the sham-operated group on the 45th day (P<0.01).
Conclusion: Feixian Formula can relieve bleomycin-induced pulmonary fibrosis in rats, and the mechanism of its action may be related to down-regulating serum PDGF.

Key words: Feixian Formula, lung diseases, interstitial, pulmonary fibrosis, transforming growth factor (31, platelet-derived growth factor, rats

CLC Number: 

  • R289.5

Table 1

Serum content of TGF-β1 in four groups ($\bar{x}$±s, ng/L)"

Group n PDGF
Day 14 Day 28 Day 45
Sham-operated 5 27.45±5.17 27.62±3.60 27.96±3.69
Untreated 8 39.00±9.11* 37.76±7.49** 50.73±10.79**
Prednisone-treated 8 42.30±9.27** 37.01±6.35* 45.00±8.80**
Feixian Formula-treated 8 39.31±8.05* 38.80±5.62** 44.24±10.77**

Table 2

Serum content of PDGF in four groups ($\bar{x}$±s, ng/L)"

Group n PDGF
Day 14 Day 28 Day 45
Sham-operated 5 19.52±4.16 18.36±2.76 19.27±8.30
Untreated 8 21.56±5.24 23.53±5.82 30.78±6.33**
Prednisone-treated 8 23.42±4.22 26.75±6.35** 29.90±4.35**
Feixian Formula-treated 8 34.48±12.70**△△▲▲ 27.08±3.73** 25.68±7.54
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