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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (8): 817-820.doi: 10.3736/jcim20080809

• Original Experimental Research • Previous Articles     Next Articles

Effects of Radix notoginseng extracts drug-containing serum on expressions of bcl-2, Bax and p21WAF1 proteins in MNNG transformed GES-1 cells

Jun-xiang Li1(), Zhi-bin Wang1, Ling-qun Zhu2, Fu-ling Niu2, Wei Cui2   

  1. 1. Department of Gastroenterology, Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China
    2. Key Laboratory of Internal Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
  • Received:2007-12-10 Online:2008-08-20 Published:2008-08-15

Objective: To investigate the effects of Radix notoginseng extracts drug-containing serum on the expressions of apoptosis-regulating proteins including Bax, bcl-2 and p21WAF1 in precancerous gastric cells.
Methods: The N-methyl-N'-nitro-N-nitroso-guanidine (MNNG) transformed eternalized human gastric mucosa epithelium GES-1 cell line (MC cell) was used in vitro as a model of gastric precancerous lesion. The medicated canine serum was prepared by feeding to the adult Beagle dog with Radix notoginseng extracts and obtaining the serum after 2-hour medication. MC cells were cultured with medicated canine serum (medicated serum group) or non-medicated canine serum (normal control group) for 72 hours. Expressions of Bax, bcl-2 and p21 WAF1 proteins were detected by immunocytochemical assay and the average optical density of the cells was determined by an image analysis system.
Results: Compared with those of the normal control group, Bax and p21 WAF1 expressions in medicated serum group were significantly enhanced (P<0.01), while the expression of bcl-2 was significantly reduced (P<0.01).
Conclusion: Radix notoginseng extracts may inhibit the proliferation and promote the apoptosis of precancerous gastric cells through altering expressions of the bcl-2, Bax and p21 WAF1 genes.

Key words: Radix notoginseng, gastric cancer, bcl-2, Bax, p21 WAF1

CLC Number: 

  • R285.5

Figure 1

Expression of bcl-2 protein in MC cells (Immunocytochemical staining, ×400) A: Normal control group; B: Medicated serum group."

Figure 2

Expression of Bax protein in MC cells (Immunocytochemical staining, ×400) A: Normal control group; B: Medicated serum group."

Figure 3

Expression of p21WAF1 protein in MC cells (Immunocytochemical staining, ×400) A: Normal control group; B: Medicated serum group."

"

Group n Optical density
bcl-2 Bax p21WAF1
Normal control 7 0.843±0.053 0.482±0.021 0.035±0.024
Medicated serum 7 0.325±0.031** 0.645±0.037** 0.246±0.028**
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