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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (6): 626-631.doi: 10.3736/jcim20110608

• Original Experimental Research • Previous Articles     Next Articles

Antinociceptive, anti-inflammatory and antidiarrheal activities of ethanolic calyx extract of Hibiscus sabdariffa Linn. (Malvaceae) in mice

Khadem Ali Md.1(),Ashraf Ayesha1,Nath Biswas Nripendra2,Kumar Karmakar Utpal2,Afroz Shamima1   

  1. 1. Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh
    2. Pharmacy Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh
  • Received:2011-02-08 Accepted:2011-03-02 Online:2011-06-20 Published:2018-10-17
  • Contact: Khadem Ali Md.

Objective: To evaluate the antinociceptive, anti-inflammatory and antidiarrheal activities of the ethanolic calyx extract of Hibiscus sabdariffa Linn. in mice.
Methods: In the present study, the dried calyxes of H. sabdariffa were subjected to extraction with 95% ethanol and the extract was used to investigate the possible activities. Antinociceptive activity of the extract was evaluated by using the acetic acid-induced writhing test. The anti-inflammatory effect of the extract was tested by using the xylene-induced ear edema model mice. Castor oil-induced diarrheal model mice were used to evaluate the antidiarrheal activity of the extract.
Results: In acetic acid-induced writhing test, the extract produced inhibited writhing in mice siginificantly compared with the blank control (P<0.01). The extract showed significant inhibition of ear edema formation in xylene-induced ear edema model mice in a dose-related manner compared with the blank control (P<0.01). The extract demonstrated a significant antidiarrheal activity against castor oil-induced diarrheal in mice in which it decreased the frequency of defecation and increased the mean latent period at the doses of 250 and 500 mg/kg body weight (P<0.01).
Conclusion: The above mentioned findings indicate that the calyx extract of H. sabdarif fa possesses significant antinociceptive, anti-inflammatory and antidiarrheal activities that support its uses in traditional medicine.

Key words: Hibiscus, plant extracts, analgesics, anti-inflammatory agents, antidiarrheals, mice

[1] Mahadevan N, Shivali, Kamboj P . Hibiscuss sabdariffa Linn. — an overview[J]. Nat Prod Rad, 2009,8(1):77-83
[2] Dahiru D, Obi OJ, Umaru H . Effect of Hibiscus sabdariffa calyx extract on carbon tetrachloride induced liver damage[J]. Biokemistri, 2003,15(1):27-33
[3] Fakeye TO, Pal A, Bawankule DU, Khanuja SP . Immunomodulatory effect of extracts of Hibiscus sabdariffa L.(Family Malvaceae) in a mouse model[J]. Phytother Res, 2008,22(5):664-668
doi: 10.1002/(ISSN)1099-1573
[4] Prommetta P, Phivthong-ngam L, Chaichantipyuth C, Niwattisaiwong N, Lawanprasert S, .Aqueous extract of the calyces of Hibiscus subdariffa Linn.: effects on hepatic cytochrome P450 and subacute toxicity in rats[J]. Thai J Pharm Sci, 2006,30(1-2):8-18
[5] Bako IG, Mabrouk MA, Abubakar A . Antioxidant effect of ethanolic seed extract of Hibiscus sabdariffa Linn(Malvaceae) alleviate the toxicity induced by chronic administration of sodium nitrate on some haematological parameters in Wistar rats[J]. Adv J Food Sci Technol, 2009,1(1):39-42
[6] Bako IG, Mohammad ST, Dawud FA, Mohammad IM, Liman AA . Hypotensive effect of ethanolic seed extract of Hibiscus sabdariffa Linn(Malvaceae) on normotensive cats[J]. Int J Pure Appl Sci, 2009,3(3):22-28
[7] Fakeye TO, Pal A, Khanuja SP . Anxiolytic and sedative effects of extracts of Hibiscus sabdariffa Linn(family Malvaceae)[J]. Afr J Med Med Sci, 2008,37(1):49-54
pmid: 18756855
[8] Reanmongkol W, Itharat A . Antipyretic activity of the extracts of Hibiscus sabdariffa calyces L. in experimental animals[J]. Songklanakarin J Sci Technol, 2007,29(Suppl 1):29-38
[9] Fakeye T . Toxicity and immunomodulatory activity of fractions of Hibiscus sabdariffa Linn(Family Malvaceae) in animal models[J]. Afr J Tradit Complement Altern Med, 2008,5(4):394-398
doi: 10.4314/ajtcam.v5i4.31296
[10] Adegunloye BJ, Omoniyi JO, Owolabi OA, Ajagbonna OP, Sofola OA, Coker HA . Mechanisms of the blood pressure lowering effect of the calyx of Hibiscus sabdariffa in rats[J]. Afr J Med Med Sci, 1996,25(3):235-238
[11] Chewonarin T, Kinouchi T, Kataoka K, Arimochi H, Kuwahara T, Vinitketkumnuen U, Ohnishi Y . Effect of roseele(Hibiscus sabdariffa Linn.), a Thai medicinal plant, on the mutagenicity of various known mutagens in Salmonella typhimurium and on formation of aberrant crypt foci induced by colon carcinogens azoxymethane and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in F344 rats[J]. Food Chem Toxicol, 1999,37(6):591-601
doi: 10.1016/S0278-6915(99)00041-1
[12] Ali MB, Salih WM, Mohamed AH, Homeida AM . Investigation on the antispasmodic potential of Hibiscus sabdariffa calyces[J]. J Ethnopharmacol, 1991,31(2):249-257
doi: 10.1016/0378-8741(91)90009-3 pmid: 2023432
[13] Olaleye MT . Cytotoxicity and antibacterial activity of methanolic extract of Hibiscus sabdariffa[J]. J Med Plant Res, 2007,1(1):9-13
[14] Zimmermann M . Ethical guidelines for investigations of experimental pain in conscious animals[J]. Pain, 1983,16(2):109-110
doi: 10.1016/0304-3959(83)90201-4 pmid: 6877845
[15] Sofowora A . Medicinal plants and traditional medicine in Africa[M]. Ibadan: Spectrum Books Ltd, 1993: 151-153
[16] Harborne JB . Phytochemical methods, a guide to modern techniques to plant analysis[M]. London: Chapman and Hall, 1998,( 3rd ed):60-66
[17] Ahmed F, Selim MS, Das AK, Choudhuri MS . Anti-inflammatory and antinociceptive activities of Lipia nodiaflora Linn[J]. Pharmazie, 2004,59(4):329-330
doi: 10.3329/dujps.v3i1.188 pmid: 15125587
[18] Deb D, Dev S, Das AK, Khanam D, Banu H, Shahriar M, Ashraf A, Choudhuri MSK, Basher SAMK . Antinociceptive, anti-inflammatory and anti-diarrheal activities of the hydroalcoholic extract of Lasia spinosa Linn.(Araceae) roots[J]. Lat Am J Pharm, 2010,29(8):1269-1276
[19] Chatterjee TK. Handbook of laboratory mice and rats[M]. Calcutta: Jadavpur University Press, 1993: 133-139
[20] Reynolds JEF, Prasad AB. Martindale: the extra pharmacopoeia [M]. London: The Pharmaceutical Press, 1982,( 28th ed):245
[21] Rang HP, Dale MM . Pharmacology[M]. Edinburgh: Churchill Livingstone, 1991,( 2nd ed):706-711
[22] Acharya SD, Ullal SD, Padiyar S, Rao YD, Upadhyaya K, Pillai D, Raj V . 大高良姜根茎提取物对小鼠的镇痛作用[J]. 中西医结合学报, 2011,9(1):100-104
Acharya SD, Ullal SD, Padiyar S, Rao YD, Upadhyaya K, Pillai D, Raj V . Analgesic effect of extracts of Alpinia galanga rhizome in mice[J]. J Chin Integr Med, 2011,9(1):100-104
[23] Ezenwali MO, Njoku OU, Okoli CO . Studies on the anti-diarrheal properties of seed extract of Monodora tenuifolia[J]. Int J Appl Res Nat Prod, 2009,2(4):20-26
[24] Mahesh GS, Patel P, Roy MPSP, Patel AN . Antidiarrheal activity of methanolic extract of Moringa oleifera Lam roots in experimental animal models[J]. Int J Pharm Research, 2010,2(2):35-39
[25] Meite S, N’guessan JD, Bahi C, Yapi HF, Djaman AJ, Guina FG . Antidiarrheal activity of the ethyl acetate extract of Morinda morindoides in rats[J]. Trop J Pharm Res, 2009,8(3):201-207
doi: 10.4314/tjpr.v8i3.44533
[26] Chakraborthy GS, Ghorpade PM . Antinociceptive activity of Abutilon indicum(Linn) sweet stem extracts[J]. Arch Pharm Sci Res, 2010,2(1):241-245
[27] Bhalke RD, Anarthe SJ, Sasane KD, Satpute SN, Shinde SN, Sangle VS . Antinociceptive activity of Trigonella foenum-graecum leaves and seeds(Fabaceae)[J]. Iran J Pharmacol Ther, 2009,8(2):57-59
[29] Zulfiker AHM, Rahman MM, Hossain MK, Hamid K, Mazumder MEH, Rana MS . In vivo analgesic activity of ethanolic extracts of two medicinal plants — Scoparia dulcis L. and Ficus racemosa Linn[J]. Biol Med, 2010,2(2):42-48
[30] Manga HM, Brkic D, Marie DE, Quetin-Leclercq J . In vivo anti-inflammatory activity of Alchornea cordifolia(Schumach. & Thonn.) Müll. Arg.(Euphorbiaceae)[J]. J Ethnopharmacol, 2004,92(2-3):209-214
doi: 10.1016/j.jep.2004.02.019
[31] Preethi KC, Kuttan G, Kuttan R . Anti-inflammatory activity of flower extract of Calendula officinalis Linn. and its possible mechanism of action[J]. Indian J Exp Biol, 2009,47(2):113-120
[32] Sanni S, Thilza IB, Talle M, Mohammed SA, Sanni FS, Okpoli LA, Jajere MS, Disa SH . The effect of Acacia nilotica pob ethyl acetate fraction on induced diarrhea in albino rats[J]. New York Sci J, 2010,3(8):16-20
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