Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (6): 595-599.doi: 10.3736/jcim20080610

• Original Experimental Research • Previous Articles     Next Articles

Influence of Feixian Formula on serum transforming growth factor-β1 and platelet-derived growth factor in rats with bleomycin-induced pulmonary fibrosis

Wei Zhang1, Liang-duo Jiang1(), Xiao-mei Zhang2, Jian-jun Wu3, Yuan Deng4, Xiang-feng Lu5   

  1. 1. Department of Respiratory Diseases, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
    2. Department of Respiratory Diseases, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
    3. Department of Traditional Chinese Medicine, Beijing Royal Integrative Medicine Hospital, Beijing 102209, China
    4. Journal of Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China
    5. Department of Pathology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
  • Received:2007-08-02 Online:2008-06-20 Published:2008-06-15
  • Contact: JIANG Liang-duo E-mail:liangduojiang@163.com

Objective: To observe the effects of Feixian Formula, a compound traditional Chinese herbal medicine for treating pulmonary fibrosis, on bleomycin-induced pulmonary fibrosis in rats, and its influence on serum transforming growth factor-β1 (TGF-β1) and platelet-derived growth factor (PDGF).
Methods: Seventy-two male Wistar rats were infused with bleomycin (1 mg/kg) through tracheal intubation to induce pulmonary fibrosis, and they were randomly divided into untreated group (n=24), prednisone-treated group (n=24) and Feixian Formula-treated group (n=24). Fifteen male Wistar rats of the sham-operated group were infused with equivalent normal saline. Twenty-four hours after operation, prednisone (5 mg/kg) and Feixian Formula (1.25 g/kg) were given to the prednisone-treated group and Feixian Formula-treated group respectively by intragastric administration once a day. Equivalent saline was administered to rats of the untreated group and sham-operated group. On the 14th, 28th and 45th day, 5 rats in the sham-operated group and 8 rats in each of the other three groups were dissected to observe pathologic changes of the lung tissues, and the levels of serum TGF-β1 and PDGF were determined by enzyme-linked immunosorbent assay.
Results: At the 45th day, the degree of pulmonary interstitial fibrosis was lesser in rats of the Feixian Formula-treated group as compared with those of the untreated group and prednisone-treated group. The levels of serum TGF-β1 and PDGF were increased, and were significantly higher than those of the sham-operated group, especially on the 45th day (P<0.05). Changes of TGF-β1 level in the prednisone-treated group and the Feixian Formula-treated group were similar to untreated group (P>0.05), and there was no significant difference between the prednisone-treated group and the Feixian Formula-treated group (P>0.05). PDGF in the Feixian Formula-treated group reached the highest level on the 14th day, significantly higher than those of the other three groups (P<0.01). Then it decreased, and was close to that of the sham-operated group on the 45th day (P=0.792). The levels of PDGF in untreated group and prednisone-treated group were increased depending on time, and were obviously higher than that of the sham-operated group on the 45th day (P<0.01).
Conclusion: Feixian Formula can relieve bleomycin-induced pulmonary fibrosis in rats, and the mechanism of its action may be related to down-regulating serum PDGF.

Key words: Feixian Formula, lung diseases, interstitial, pulmonary fibrosis, transforming growth factor (31, platelet-derived growth factor, rats

CLC Number: 

  • R289.5

Table 1

Serum content of TGF-β1 in four groups ($\bar{x}$±s, ng/L)"

Group n PDGF
Day 14 Day 28 Day 45
Sham-operated 5 27.45±5.17 27.62±3.60 27.96±3.69
Untreated 8 39.00±9.11* 37.76±7.49** 50.73±10.79**
Prednisone-treated 8 42.30±9.27** 37.01±6.35* 45.00±8.80**
Feixian Formula-treated 8 39.31±8.05* 38.80±5.62** 44.24±10.77**

Table 2

Serum content of PDGF in four groups ($\bar{x}$±s, ng/L)"

Group n PDGF
Day 14 Day 28 Day 45
Sham-operated 5 19.52±4.16 18.36±2.76 19.27±8.30
Untreated 8 21.56±5.24 23.53±5.82 30.78±6.33**
Prednisone-treated 8 23.42±4.22 26.75±6.35** 29.90±4.35**
Feixian Formula-treated 8 34.48±12.70**△△▲▲ 27.08±3.73** 25.68±7.54
[1] Zhang W, Lu XF, Zhang XM , et al. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation[J]. Zhong Xi Yi Jie He Xue Bao, 2008,6(1):60-67
张伟, 鲁香凤, 张晓梅 , 等. 气管插管快速灌注博莱霉素复制大鼠肺间质纤维化模型[J]. 中西医结合学报, 2008,6(1):60-67
[2] Yi XH . ATS/ERS classification and pathologic diagnosis of the idiopathic interstitial pneumonia[J]. Zhen Duan Bing Li Xue Za Zhi, 2005,12(6):401-404
易祥华 . 介绍特发性间质性肺炎的ATS/ERS分类及病理诊断[J]. 诊断病理学杂志, 2005,12(6):401-404
[3] Hou XM, Yu RJ. Interstitial pulmonology[M]. Beijing: People's Medical Publishing House, 2003: 33-35
侯显明, 于润江 . 间质性肺病学[M]. 北京: 人民卫生出版社, 2003: 33-35
[4] Iyer SN, Gurujeyalakshmi G, Giri SN . Effect of pirfenidone on procollagen gene expression at the transcriptional level in bleomycin hamster model of lung fibrosis[J]. J Pharmacol Exp Ther, 1999,289(1):211-218
[5] Van Hoozen BE, Grimmer KL, Marelich GP , et al. Early phase collagen synjournal in lungs of rats exposed to bleomycin[J]. Toxicology, 2000,147(1):1-13
doi: 10.1016/S0300-483X(00)00142-6 pmid: 10837927
[6] Hou XM, Yu RJ. Interstitial pulmonology[M]. Beijing: People's Medical Publishing House, 2003: 46
侯显明, 于润江 . 间质性肺病学[M]. 北京: 人民卫生出版社, 2003: 46
[7] Ludwicka A, Ohba T, Trojanowska M , et al. Elevated levels of platelet derived growth factor and transforming growth factor-beta 1 in bronchoalveolar lavage fluid from patients with scleroderma[J]. J Rheumatol, 1995,22(10):1876-1883
[8] Melloni B, Lesur O, Bouhadiba T , et al. Effect of exposure to silica on human alveolar macrophages in supporting growth activity in typeⅡepithelial cells[J]. Thorax, 1996,51(8):781-786
doi: 10.1136/thx.51.8.781
[9] Wu H, Zhang YE, Xu ZD , et al. Influence of platelet-derived growth factor on the synjournal of procollagen in rat pulmonary fibroblasts[J]. Shanghai Yi Ke Da Xue Xue Bao, 1996,23(3):199-202
doi: 10.1007/BF02951625
吴浩, 张月娥, 许祖德 , 等. 血小板源性生长因子对肺成纤维母细胞前胶原合成的影响[J]. 上海医科大学学报, 1996,23(3):199-202
doi: 10.1007/BF02951625
[1] Na Zhi, Qian Mo, Shuo Yang, Yuan-xing Qin, Hao Chen, Zeng-guang Wu, Cai-hong Lan, Jun Zhang, Yin-long Li. Treatment of pulmonary fibrosis in one convalescent patient with corona virus disease 2019 by oral traditional Chinese medicine decoction: A case report. Journal of Integrative Medicine, 2021, 19(2): 185-190.
[2] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[3] Qing-lan Wang, Yan-yan Tao, Hong-dong Xie, Cheng-hai Liu, Ping Liu. Fuzheng Huayu recipe, a traditional Chinese compound herbal medicine, attenuates renal interstitial fibrosis via targeting the miR-21/PTEN/AKT axis. Journal of Integrative Medicine, 2020, 18(6): 505-513.
[4] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[5] Xue-qing Yu, Shu-guang Yang, Yang Xie, Jian-sheng Li. Traditional Chinese medicine in the treatment of idiopathic pulmonary fibrosis based on syndrome differentiation: Study protocol of exploratory trial. Journal of Integrative Medicine, 2020, 18(2): 163-168.
[6] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[7] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[8] Hoda Mojiri-Forushani, Ali Asghar Hemmati, Mohammad Amin Dehghani, Ali Reza Malayeri, Hossein Hassan Pour. Effects of herbal extracts and compounds and pharmacological agents on pulmonary fibrosis in animal models: A review. Journal of Integrative Medicine, 2017, 15(6): 433-441.
[9] JiaXia, Li-qunHe, XiaoSu. Interventional mechanisms of herbs or herbal extracts on renal interstitial fibrosis. Journal of Integrative Medicine, 2016, 14(3): 165-173.
[10] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[11] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[12] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[13] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[14] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[15] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239